Proteinase Activated Receptor 3 (F2RL2) (NM_001256566) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PAR-3; PAR3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232557 representing NM_001256566 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAATGATACAAACAACTTGGCAAAGCCAACCTTACCCATTAAGACCTTTCGTGGAGCTCCCCCAA ATTCTTTTGAAGAGTTCCCCTTTTCTGCCTTGGAAGGCTGGACAGGAGCCACGATTACTGTAAAAATTAA GTGCCCTGAAGAAAGTGCTTCACATCTCCATGTGAAAAATGCTACCATGGGGTACCTGACCAGCTCCTTA AGTACTAAACTGATACCTGCCATCTACCTCCTGGTGTTTGTAGTTGGTGTCCCGGCCAATGCTGTGACCC TGTGGATGCTTTTCTTCAGGACCAGATCCATCTGTACCACTGTATTCTACACCAACCTGGCCATTGCAGA TTTTCTTTTTTGTGTTACATTGCCCTTTAAGATAGCTTATCATCTCAATGGGAACAACTGGGTATTTGGA GAGGTCCTGTGCCGGGCCACCACAGTCATCTTCTATGGCAACATGTACTGCTCCATTCTGCTCCTTGCCT GCATCAGCATCAACCGCTACCTGGCCATCGTCCATCCTTTCACCTACCGGGGCCTGCCCAAGCACACCTA TGCCTTGGTAACATGTGGACTGGTGTGGGCAACAGTTTTCTTATATATGCTGCCATTTTTCATACTGAAG CAGGAATATTATCTTGTTCAGCCAGACATCACCACCTGCCATGATGTTCACAACACTTGCGAGTCCTCAT CTCCCTTCCAACTCTATTACTTCATCTCCTTGGCATTCTTTGGATTCTTAATTCCATTTGTGCTTATCAT CTACTGCTATGCAGCCATCATCCGGACACTTAATGCATACGATCATAGATGGTTGTGGTATGTTAAGGCG AGTCTCCTCATCCTTGTGATTTTTACCATTTGCTTTGCTCCAAGCAATATTATTCTTATTATTCACCATG CTAACTACTACTACAACAACACTGATGGCTTATATTTTATATATCTCATAGCTTTGTGCCTGGGTAGTCT TAATAGTTGCTTAGATCCATTCCTTTATTTTCTCATGTCAAAAACCAGAAATCACTCCACTGCTTACCTT ACAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232557 representing NM_001256566 Red=Cloning site Green=Tags(s) MENDTNNLAKPTLPIKTFRGAPPNSFEEFPFSALEGWTGATITVKIKCPEESASHLHVKNATMGYLTSSL STKLIPAIYLLVFVVGVPANAVTLWMLFFRTRSICTTVFYTNLAIADFLFCVTLPFKIAYHLNGNNWVFG EVLCRATTVIFYGNMYCSILLLACISINRYLAIVHPFTYRGLPKHTYALVTCGLVWATVFLYMLPFFILK QEYYLVQPDITTCHDVHNTCESSSPFQLYYFISLAFFGFLIPFVLIIYCYAAIIRTLNAYDHRWLWYVKA SLLILVIFTICFAPSNIILIIHHANYYYNNTDGLYFIYLIALCLGSLNSCLDPFLYFLMSKTRNHSTAYL TK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256566
ORF Size	1056 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256566.2
RefSeq Size	3295 bp
RefSeq ORF	1059 bp
Locus ID	2151
UniProt ID	O00254
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction
MW	40.7 kDa
Gene Summary	This gene encodes a member of the protease-activated receptor (PAR) family which is a subfamily of the seven transmembrane G protein-coupled cell surface receptor family. The encoded protein acts as a cofactor in the thrombin-mediated cleavage and activation of the protease-activated receptor family member PAR4. The encoded protein plays an essential role in hemostasis and thrombosis. Alternate splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Feb 2012]

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