Proteinase Activated Receptor 3 (F2RL2) (NM_001256566) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PAR-3; PAR3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC330455 representing NM_001256566. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAAATGATACAAACAACTTGGCAAAGCCAACCTTACCCATTAAGACCTTTCGTGGAGCTCCCCCA AATTCTTTTGAAGAGTTCCCCTTTTCTGCCTTGGAAGGCTGGACAGGAGCCACGATTACTGTAAAAATT AAGTGCCCTGAAGAAAGTGCTTCACATCTCCATGTGAAAAATGCTACCATGGGGTACCTGACCAGCTCC TTAAGTACTAAACTGATACCTGCCATCTACCTCCTGGTGTTTGTAGTTGGTGTCCCGGCCAATGCTGTG ACCCTGTGGATGCTTTTCTTCAGGACCAGATCCATCTGTACCACTGTATTCTACACCAACCTGGCCATT GCAGATTTTCTTTTTTGTGTTACATTGCCCTTTAAGATAGCTTATCATCTCAATGGGAACAACTGGGTA TTTGGAGAGGTCCTGTGCCGGGCCACCACAGTCATCTTCTATGGCAACATGTACTGCTCCATTCTGCTC CTTGCCTGCATCAGCATCAACCGCTACCTGGCCATCGTCCATCCTTTCACCTACCGGGGCCTGCCCAAG CACACCTATGCCTTGGTAACATGTGGACTGGTGTGGGCAACAGTTTTCTTATATATGCTGCCATTTTTC ATACTGAAGCAGGAATATTATCTTGTTCAGCCAGACATCACCACCTGCCATGATGTTCACAACACTTGC GAGTCCTCATCTCCCTTCCAACTCTATTACTTCATCTCCTTGGCATTCTTTGGATTCTTAATTCCATTT GTGCTTATCATCTACTGCTATGCAGCCATCATCCGGACACTTAATGCATACGATCATAGATGGTTGTGG TATGTTAAGGCGAGTCTCCTCATCCTTGTGATTTTTACCATTTGCTTTGCTCCAAGCAATATTATTCTT ATTATTCACCATGCTAACTACTACTACAACAACACTGATGGCTTATATTTTATATATCTCATAGCTTTG TGCCTGGGTAGTCTTAATAGTTGCTTAGATCCATTCCTTTATTTTCTCATGTCAAAAACCAGAAATCAC TCCACTGCTTACCTTACAAAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001256566
Insert Size	1059 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256566.1
RefSeq Size	3295 bp
RefSeq ORF	1059 bp
Locus ID	2151
UniProt ID	O00254
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction
MW	40.2 kDa
Gene Summary	This gene encodes a member of the protease-activated receptor (PAR) family which is a subfamily of the seven transmembrane G protein-coupled cell surface receptor family. The encoded protein acts as a cofactor in the thrombin-mediated cleavage and activation of the protease-activated receptor family member PAR4. The encoded protein plays an essential role in hemostasis and thrombosis. Alternate splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (2) uses an alternate exon in the 5' UTR and coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1.

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