MFSD11 (NM_001242534) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ET
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232746 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCCGGAATCTAAAAAGCTTTTCAACATCATTATTTTAGGAGTTGCCTTTATGTTTATGTTCACTG CCTTTCAAACTTGTGGAAATGTGGCGCAAACTGTCATCAGGAGCTTAAATAGGACAGATTTTCACGGCAG TGGATATACCAGCATGGCTATTATCTATGGAGTGTTCTCTGCTTCAAATTTGATTACACCGTCAGTGGTT GCCATTGTAGGACCTCAACTCTCTATGTTTGCCAGTGGTTTATTTTACAGCATGTACATTGCCGTTTTCA TCCAGCCTTTCCCGTGGTCCTTCTACACAGCCTCTGTTTTCATTGGAATTGCTGCTGCTGTGCTTTGGAC AGCACAAGGAAACTGCCTGACAATCAATTCGGATGAGCACAGCATTGGGAGAAACAGTGGGATTTTCTGG GCACTTCTGCAGTCTAGCTTGTTCTTTGGAAATCTCTACATATATTTTGCCTGGCAAGGGAAAACTCAGA TATCAGAGAGTGACCGAAGAACAGTGTTTATTGCCCTAACGGTGATTAGCCTTGTGGGGACAGTTCTATT CTTTCTCATTCGGAAACCAGATTCTGAAAATGTCCTAGGAGAAGATGAGTCTTCTGATGACCAGGACATG GAAGTCAACGAGTCTGCCCAGAACAATCTGACAAAGGCAGTAGATGCTTTTAAAAAGTCTTTTAAGTTAT GTGTCACCAAGGAGATGCTCCTTCTTAGTATTACAACTGCTTATACAGGTCTGGAATTAACTTTCTTCTC TGGTGTATATGGAACCTGTATTGGTGCTACAAATAAATTTGGAGCAGAAGAGAAAAGCCTTATTGGACTT TCTGGCATTTTCATCGGCATTGGAGAAATTTTAGGTGGAAGCCTCTTCGGCCTGCTGAGCAAGAACAATC GTTTTGGTAGAAATCCAGTTGTGCTGTTGGGCATCCTGGTGCACTTCATAGCTTTTTATCTAATATTTCT CAACATGCCTGGAGATGCCCCGATTGCTCCTGTTAAAGGAACTGACAGCAGTGCTTACATCAAATCCAGC AAAGAAGTTGCCATTCTCTGCAGTTTTCTGTTGGGCCTTGGAGACAGCTGCTTTAATACCCAGCTGCTTA GTATCTTGGGCTTTCTGTATTCTGAAGACAGCGCCCCAGCATTTGCCATCTTCAAGTTTGTTCAGTCTAT TTGCGCAGCCGTGGCATTTTTCTACAGCAACTACCTTCTCCTTCACTGGCAACTCCTGGTCATGGTGATA TTTGGGTTTTTTGGAACAATTTCTTTCTTCACTGTGGAATGGGAAGCTGCCGCCTTTGTAGCCCGCGGCT CTGACTACCGAAGTATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232746 protein sequence Red=Cloning site Green=Tags(s) MSPESKKLFNIIILGVAFMFMFTAFQTCGNVAQTVIRSLNRTDFHGSGYTSMAIIYGVFSASNLITPSVV AIVGPQLSMFASGLFYSMYIAVFIQPFPWSFYTASVFIGIAAAVLWTAQGNCLTINSDEHSIGRNSGIFW ALLQSSLFFGNLYIYFAWQGKTQISESDRRTVFIALTVISLVGTVLFFLIRKPDSENVLGEDESSDDQDM EVNESAQNNLTKAVDAFKKSFKLCVTKEMLLLSITTAYTGLELTFFSGVYGTCIGATNKFGAEEKSLIGL SGIFIGIGEILGGSLFGLLSKNNRFGRNPVVLLGILVHFIAFYLIFLNMPGDAPIAPVKGTDSSAYIKSS KEVAILCSFLLGLGDSCFNTQLLSILGFLYSEDSAPAFAIFKFVQSICAAVAFFYSNYLLLHWQLLVMVI FGFFGTISFFTVEWEAAAFVARGSDYRSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242534
ORF Size	1347 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242534.2
RefSeq Size	2850 bp
RefSeq ORF	1350 bp
Locus ID	79157
UniProt ID	O43934
Protein Families	Transmembrane
MW	49.2 kDa

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