ELMO1 (NM_001206482) Human Tagged ORF Clone
CAT#: RC233106
- TrueORF®
ELMO1 (Myc-DDK tagged) - Homo sapiens engulfment and cell motility 1 (ELMO1), transcript variant 5
ORF Plasmid: tGFP
"NM_001206482" in other vectors (2)
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CNY 5,320.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CED-12; CED12; ELMO-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC233106 representing NM_001206482.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCGCCACCCGCGGACATCGTCAAGGTGGCCATAGAATGGCCGGGCGCCTACCCCAAACTCATGGAA ATTGATCAGAAAAAACCACTGTCTGCAATAATAAAGGAAGTCTGTGATGGGTGGTCTCTTGCCAACCAT GAATATTTTGCACTCCAGCATGCCGATAGTTCAAACTTCTATATCACAGAAAAGAACCGCAATGAGATA AAAAATGGCACTATCCTTCGATTAACCACATCTCCAGCTCAGAACGCCCAGCAGCTCCATGAACGAATC CAGTCCTCGAGTATGGATGCCAAGCTGGAAGCCCTGAAGGACTTGGCCAGCCTCTCCCGGGATGTCACG TTTGCCCAGGAGTTTATAAACCTGGACGGTATCTCTCTCCTCACGCAGATGGTAGAGAGCGGCACTGAG CGATACCAGAAATTGCAGAAGATCATGAAGCCTTGCTTTGGAGACATGCTGTCCTTCACCCTGACGGCC TTCGTTGAGCTGATGGACCATGGCATAGTGTCCTGGGATACATTTTCGGTGGCGTTCATTAAGAAGATA GCAAGTTTTGTGAACAAGTCAGCCATAGACATCTCGATCCTGCAGCGGTCCTTGGCCATTTTGGAGTCG ATGGTGCTCAATAGCCATGACCTCTACCAGAAAGTGGCGCAGGAGATCACCATCGGCCAGCTCATTCCA CACCTGCAAGGGTCAGATCAAGAAATCCAAACCTATACTATTGCAGTGATTAATGCGCTTTTCCTGAAG GCTCCTGATGAGAGGAGGCAGGAGATGGCGAATATTTTGGCTCAGAAGCAACTGCGTTCCATCATTTTA ACACATGTCATCCGAGCCCAGCGGGCCATCAACAATGAGATGGCGCACCAGCTGTATGTTCTACAAGTG CTCACCTTTAACCTCCTGGAAGACAGGATGATGACCAAAATGGACCCCCAGGACCAGGCTCAGAGGGAC ATCATATTTGAACTTCGAAGAATTGCTTTTGATGCTGAGTCTGAACCTAACAACAGCAGTGGCAGCATG GAGAAACGCAAGTCCATGTACACGCGAGATTATAAGAAGCTTGGGTTCATTAATCATGTCAACCCTGCC ATGGACTTCACGCAGACTCCACCTGGGATGTTGGCTCTGGACAACATGCTGTACTTTGCCAAGCACCAC CAAGATGCCTACATCCGGATTGTGCTTGAGAACAGTAGTCGAGAAGACAAGCATGAATGTCCCTTTGGC CGCAGTAGTATAGAGCTGACCAAGATGCTATGTGAGATCTTGAAAGTGGGCGAGTTGCCTAGTGAGACC TGCAACGACTTCCACCCGATGTTCTTCACCCACGACAGATCCTTTGAGGAGTTTTTCTGCATCTGTATC CAGCTCCTGAACAAGACATGGAAGGAAATGAGGGCAACTTCTGAAGACTTCAACAAGGTAATGCAGGTG GTGAAGGAGCAGGTTATGAGAGCACTTACAACCAAGCCTAGCTCCCTGGACCAGTTCAAGAGCAAACTG CAGAACCTGAGCTACACTGAGATCCTGAAAATCCGCCAGTCCGAGAGGATGAACCAGGAAGATTTCCAG TCCCGCCCGATTTTGGAACTAAAGGAGAAGATTCAGCCAGAAATCTTAGAGCTGATCAAACAGCAACGC CTGAACCGCCTTGTGGAAGGGACCTGCTTTAGGAAACTCAATGCCCGGCGGAGGCAAGACAAGTTTTGG TATTGTCGGCTTTCGCCAAATCACAAAGTCCTGCATTACGGAGACTTAGAAGAGAGTCCTCAGGGAGAA GTGCCCCACGATTCCTTGCAGGACAAACTGCCGGTGGCAGATATCAAAGCCGTGGTGACGGGAAAGGAC TGCCCTCATATGAAAGAGAAAGGTGCCCTTAAACAAAACAAGGAGGTGCTTGAACTCGCTTTCTCCATC TTGTATGACTCAAACTGCCAACTGAACTTCATCGCTCCTGACAAGCATGAGTACTGTATCTGGACGGAT GGACTGAATGCGCTACTCGGGAAGGACATGATGAGCGACCTGACGCGGAATGACCTGGACACCCTGCTC AGCATGGAAATCAAGCTCCGCCTCCTGGACCTGGAAAACATCCAGATCCCTGACGCACCTCCGCCGATT CCCAAGGAGCCCAGCAACTATGACTTCGTCTATGACTGTAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC233106
Blue=ORF Red=Cloning site Green=Tag(s) MPPPADIVKVAIEWPGAYPKLMEIDQKKPLSAIIKEVCDGWSLANHEYFALQHADSSNFYITEKNRNEI KNGTILRLTTSPAQNAQQLHERIQSSSMDAKLEALKDLASLSRDVTFAQEFINLDGISLLTQMVESGTE RYQKLQKIMKPCFGDMLSFTLTAFVELMDHGIVSWDTFSVAFIKKIASFVNKSAIDISILQRSLAILES MVLNSHDLYQKVAQEITIGQLIPHLQGSDQEIQTYTIAVINALFLKAPDERRQEMANILAQKQLRSIIL THVIRAQRAINNEMAHQLYVLQVLTFNLLEDRMMTKMDPQDQAQRDIIFELRRIAFDAESEPNNSSGSM EKRKSMYTRDYKKLGFINHVNPAMDFTQTPPGMLALDNMLYFAKHHQDAYIRIVLENSSREDKHECPFG RSSIELTKMLCEILKVGELPSETCNDFHPMFFTHDRSFEEFFCICIQLLNKTWKEMRATSEDFNKVMQV VKEQVMRALTTKPSSLDQFKSKLQNLSYTEILKIRQSERMNQEDFQSRPILELKEKIQPEILELIKQQR LNRLVEGTCFRKLNARRRQDKFWYCRLSPNHKVLHYGDLEESPQGEVPHDSLQDKLPVADIKAVVTGKD CPHMKEKGALKQNKEVLELAFSILYDSNCQLNFIAPDKHEYCIWTDGLNALLGKDMMSDLTRNDLDTLL SMEIKLRLLDLENIQIPDAPPPIPKEPSNYDFVYDCN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001206482 |
ORF Size | 2181 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 5539 bp |
RefSeq ORF | 2184 bp |
Locus ID | 9844 |
UniProt ID | Q92556 |
Protein Pathways | Chemokine signaling pathway |
MW | 83.8 kDa |
Gene Summary | This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
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