DATF1 (DIDO1) (NM_001193370) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BYE1; C20orf158; DATF-1; DATF1; DIDO2; DIDO3; DIO-1; DIO1; dJ885L7.8
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233206 representing NM_001193370 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGACAAAGGCGACCCGAGCAATGAGGAGGCACCTAAGGCCATCAAACCCACCAGCAAAGAGTTCA GGAAAACATGGGGTTTTCGAAGGACCACTATCGCCAAGCGAGAGGGCGCAGGGGACGCGGAGGCTGACCC ACTGGAGCCGCCACCCCCACAGCAGCAGCTGGGCCTGTCCCTGCGGCGCAGTGGGAGGCAGCCCAAGCGC ACTGAGCGCGTGGAGCAGTTCCTGACCATTGCGCGGCGCCGCGGCAGGAGGAGCATGCCTGTCTCCCTGG AGGATTCTGGTGAGCCCACGTCCTGCCCCGCCACAGACGCCGAGACAGCCTCCGAGGGCAGCGTGGAAAG CGCTTCTGAGACCAGAAGCGGCCCCCAGTCTGCTTCCACAGCTGTGAAGGAACGACCAGCCTCTTCTGAA AAGGTGAAAGGAGGGGATGACCACGATGACACCTCCGATAGTGACAGCGATGGCCTGACCTTGAAAGAGC TTCAGAATCGCCTTCGCAGGAAGCGGGAACAGGAGCCCACTGAGAGGCCCCTGAAAGGGATCCAGAGTCG CCTGCGGAAGAAGCGCCGGGAGGAGGGTCCCGCCGAGACTGTGGGCTCCGAGGCCAGTGACACTGTGGAG GGCGTCCTGCCCAGTAAGCAGGAGCCCGAGAACGATCAGGGGGTTGTGTCCCAGGCTGGGAAAGATGACA GAGAGAGTAAGTTGGAGGGAAAGGCGGCTCAGGACATCAAAGATGAGGAGCCTGGAGACTTGGGCCGACC GAAGCCTGAATGTGAGGGTTACGACCCCAACGCCCTGTATTGCATTTGCCGCCAGCCTCACAACAACAGG TTTATGATTTGCTGTGACCGCTGTGAAGAATGGTTTCATGGCGATTGTGTGGGCATTTCTGAGGCTCGAG GGAGGCTTTTGGAAAGGAATGGGGAAGACTATATCTGCCCAAACTGCACCATTCTGCAAGTGCAGGATGA GACTCATTCAGAAACGGCAGATCAGCAGGAAGCTAAATGGAGACCTGGAGATGCTGATGGCACCGATTGT ACAAGTATAGGAACAATAGAGCAGAAGTCTAGCGAAGACCAAGGGATAAAGGGTAGAATTGAGAAAGCTG CAAATCCAAGTGGCAAGAAGAAACTCAAGATCTTCCAGCCTGTGATAGAGGCGCCTGGTGCCTCAAAATG TATTGGCCCCGGGTGCTGTCACGTGGCGCAGCCCGACTCGGTGTACTGCAGTAATGACTGTATCCTCAAA CACGCCGCAGCGACAATGAAGTTTCTAAGCTCAGGTAAAGAACAGAAGCCAAAGCCTAAAGAAAAGATGA AGATGAAGCCAGAGAAGCCCAGTCTTCCGAAATGCGGTGCTCAGGCAGGTATTAAAATCTCTTCTGTGCA CAAGAGACCAGCTCCAGAAAAAAAAGAGACCACAGTGAAGAAGGCAGTGGTGGTCCCTGCGCGGAGTGAA GCACTCGGGAAGGAAGCAGCTTGTGAGAGCAGCACGCCGTCGTGGGCGAGCGATCACAATTACAATGCAG TAAAGCCAGAAAAGACTGCTGCTCCCTCGCCGTCACTGTTGTATAAATCCACGAAGGAAGACAGGAGGTC CGAGGAGAAAGCGGCAGCCATGGCAGCCTCAAAGAAAACAGCCCCTCCAGGCTCCGCGGTGGGCAAGCAG CCTGCACCTAGAAACCTCGTGCCAAAGAAGTCTTCTTTTGCTAATGTGGCAGCAGCCACACCAGCCATTA AAAAGCCACCCTCAGGTTTCAAGGGCACCATCCCCAAGAGGCCATGGCTCTCCGCTACCCCATCGAGTGG TGCTTCAGCTGCCAGGCAGGCCGGACCGGCACCTGCAGCGGCAACGGCTGCCTCCAAGAAGTTCCCTGGC TCCGCTGCTTTGGTGGGAGCCGTAAGGAAGCCAGTGGTACCTTCTGTTCCAATGGCCTCGCCAGCCCCAG GACGCCTTGGGGCTATGAGTGCTGCACCATCGCAGCCAAATTCACAAATTCGGCAAAATATCAGACGCTC CTTAAAAGAGATTTTGTGGAAAAGAGTCAATGACAGCGATGACTTAATCATGACAGAAAACGAAGTAGGA AAAATTGCCCTCCATATTGAGAAGGAGATGTTTAACTTGTTTCAAGTTACAGATAATCGCTACAAGAGTA AATATCGCAGCATCATGTTCAACCTGAAGGACCCTAAAAATCAGGGACTCTTCCATCGTGTTCTGCGTGA GGAAATCTCTTTGGCGAAACTTGTGAGACTGAAGCCAGAAGAACTTGTATCTAAAGAGCTTTCCACGTGG AAAGAGAGGCCAGCGAGATCTGTGATGGAGTCCAGAACTAAACTGCACAATGAAAGCAAGAAGACGGCCC CCAGGCAGGAGGCCATCCCCGATCTGGAGGACTCTCCGCCAGTGTCGGATTCAGAGGAACAGCAAGAGTC AGCACGTGCTGTCCCTGAGAAGAGCACAGCGCCGCTTCTCGACGTCTTCAGCAGCATGTTGAAAGACACC ACCAGTCAGCACCGCGCACACCTCTTCGATCTCAACTGTAAAATTTGCACAGGCCAGGTTCCCTCCGCAG AAGATGAGCCAGCTCCGAAAAAACAAAAATTGTCAGCTTCTGTTAAGAAAGAAGACTTAAAATCAAAGCA TGACAGCTCTGCACCTGACCCAGCTCCGGATTCAGCTGATGAGGTGATGCCGGAGGCTGTGCCTGAAGTT GCCTCTGAGCCAGGCCTAGAAAGTGCTTCTCATCCAAATGTGGACAGAACGTATTTCCCTGGGCCTCCAG GAGATGGCCATCCCGAGCCCTCCCCGCTGGAAGACCTGTCCCCCTGCCCAGCCTCCTGTGGGAGCGGGGT GGTCACCACCGTCACAGTGTCCGGCCGGGACCCCAGGACCGCTCCAAGCAGTTCATGCACAGCCGTGGCC TCCGCAGCATCCCGCCCAGACAGCACCCACATGGTGGAAGCCAGACAGGATGTGCCGAAGCCTGTCTTGA CTTCTGTGATGGTGCCCAAGTCCATACTAGCTAAGCCATCCTCATCTCCTGACCCAAGATACCTGTCAGT TCCTCCGTCACCAAATATCAGCACTTCAGAATCACGTTCCCCTCCAGAGGGAGACACGACCCTCTTTTTG TCTCGACTCAGCACCATTTGGAAAGGATTTATTAACATGCAGAGTGTGGCAAAATTTGTCACTAAGGCGT ATCCTGTCTCTGGGTGTTTTGATTACCTCAGTGAGGATTTGCCTGACACAATTCACATTGGTGGGAGGAT CGCACCGAAGACAGTTTGGGATTATGTTGGCAAACTCAAGTCTTCTGTGTCTAAGGAGCTCTGTCTGATC CGCTTCCACCCCGCCACAGAGGAAGAGGAGGTCGCCTATATCTCTCTCTACTCCTATTTCAGCAGCCGTG GCCGCTTTGGTGTTGTAGCTAATAACAACAGGCACGTCAAGGACCTCTACCTGATCCCGCTGAGCGCCCA GGACCCTGTTCCATCCAAACTCTTGCCCTTTGAGGGACCAGGTAAGCGCCGGCTTTCTGGGTGGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233206 representing NM_001193370 Red=Cloning site Green=Tags(s) MDDKGDPSNEEAPKAIKPTSKEFRKTWGFRRTTIAKREGAGDAEADPLEPPPPQQQLGLSLRRSGRQPKR TERVEQFLTIARRRGRRSMPVSLEDSGEPTSCPATDAETASEGSVESASETRSGPQSASTAVKERPASSE KVKGGDDHDDTSDSDSDGLTLKELQNRLRRKREQEPTERPLKGIQSRLRKKRREEGPAETVGSEASDTVE GVLPSKQEPENDQGVVSQAGKDDRESKLEGKAAQDIKDEEPGDLGRPKPECEGYDPNALYCICRQPHNNR FMICCDRCEEWFHGDCVGISEARGRLLERNGEDYICPNCTILQVQDETHSETADQQEAKWRPGDADGTDC TSIGTIEQKSSEDQGIKGRIEKAANPSGKKKLKIFQPVIEAPGASKCIGPGCCHVAQPDSVYCSNDCILK HAAATMKFLSSGKEQKPKPKEKMKMKPEKPSLPKCGAQAGIKISSVHKRPAPEKKETTVKKAVVVPARSE ALGKEAACESSTPSWASDHNYNAVKPEKTAAPSPSLLYKSTKEDRRSEEKAAAMAASKKTAPPGSAVGKQ PAPRNLVPKKSSFANVAAATPAIKKPPSGFKGTIPKRPWLSATPSSGASAARQAGPAPAAATAASKKFPG SAALVGAVRKPVVPSVPMASPAPGRLGAMSAAPSQPNSQIRQNIRRSLKEILWKRVNDSDDLIMTENEVG KIALHIEKEMFNLFQVTDNRYKSKYRSIMFNLKDPKNQGLFHRVLREEISLAKLVRLKPEELVSKELSTW KERPARSVMESRTKLHNESKKTAPRQEAIPDLEDSPPVSDSEEQQESARAVPEKSTAPLLDVFSSMLKDT TSQHRAHLFDLNCKICTGQVPSAEDEPAPKKQKLSASVKKEDLKSKHDSSAPDPAPDSADEVMPEAVPEV ASEPGLESASHPNVDRTYFPGPPGDGHPEPSPLEDLSPCPASCGSGVVTTVTVSGRDPRTAPSSSCTAVA SAASRPDSTHMVEARQDVPKPVLTSVMVPKSILAKPSSSPDPRYLSVPPSPNISTSESRSPPEGDTTLFL SRLSTIWKGFINMQSVAKFVTKAYPVSGCFDYLSEDLPDTIHIGGRIAPKTVWDYVGKLKSSVSKELCLI RFHPATEEEEVAYISLYSYFSSRGRFGVVANNNRHVKDLYLIPLSAQDPVPSKLLPFEGPGKRRLSGWR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001193370
ORF Size	3567 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001193370.2
RefSeq Size	7613 bp
RefSeq ORF	3570 bp
Locus ID	11083
UniProt ID	Q9BTC0
Protein Families	Druggable Genome, Transcription Factors
MW	129.6 kDa
Gene Summary	Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms. [provided by RefSeq, Jul 2008]

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