CLASP2 (NM_001207044) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233210 representing NM_001207044 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTATGGGAGATGATAAAAGCTTCGATGATGAAGAATCAGTGGATGGAAATAGGCCATCATCAGCTG CATCAGCCTTCAAGGTTCCTGCACCTAAAACATCCGGAAATCCTGCCAACAGTGCAAGGAAGCCTGGTTC AGCAGGTGGCCCTAAGGTTGGAGGTGCTTCTAAGGAAGGAGGTGCTGGAGCAGTTGATGAAGATGATTTT ATAAAAGCTTTTACAGATGTCCCTTCTATTCAGATTTATTCTAGTCGAGAACTCGAAGAAACATTAAATA AAATCAGGGAAATTTTGTCAGATGATAAACATGACTGGGATCAGCGTGCCAATGCACTGAAGAAAATTCG ATCACTGCTTGTTGCTGGAGCTGCACAGTATGATTGCTTTTTTCAACATTTACGATTGTTGGATGGAGCA CTTAAACTTTCAGCTAAGGATCTTAGATCCCAGGTGGTTAGAGAAGCTTGTATTACTGTAGCCCACCTTT CAACAGTTTTGGGAAACAAGTTTGATCATGGCGCTGAAGCCATTGTACCTACACTTTTTAATCTCGTCCC CAATAGTGCAAAAGTCATGGCAACTTCTGGATGTGCAGCAATCAGATTTATCATTCGGCATACTCATGTA CCCAGACTTATACCTTTAATAACAAGCAATTGCACATCAAAATCAGTTCCCGTGAGGAGACGTTCATTTG AATTTTTAGATTTATTGTTGCAAGAGTGGCAGACTCATTCATTGGAAAGACATGCAGCCGTCTTGGTTGA AACTATTAAAAAGGGAATTCATGATGCTGACGCTGAGGCCAGAGTGGAGGCAAGAAAGACATACATGGGT CTTAGAAACCACTTTCCTGGTGAAGCTGAAACATTATATAATTCCCTTGAGCCATCTTATCAGAAGAGTC TTCAAACTTACTTAAAGAGTTCTGGCAGTGTAGCATCTCTTCCACAATCAGACAGGTCCTCATCCAGCTC ACAGGAAAGTCTCAATCGCCCTTTTTCTTCCAAATGGTCTACAGCAAATCCATCAACTGTGGCTGGAAGA GTATCAGCAGGCAGCAGCAAAGCCAGTTCCCTTCCAGGAAGCCTGCAGCGTTCACGAAGTGACATTGATG TGAATGCTGCTGCAGGTGCCAAGGCACATCATGCTGCTGGACAGTCTGTGCGAAGCGGGCGCTTAGGTGC AGGTGCCCTGAATGCAGGTTCCTATGCGTCACTAGAGGATACTTCTGACAAGCTGGATGGAACAGCATCT GAAGATGGCCGGGTGAGAGCAAAACTTTCAGCACCACTTGCTGGCATGGGAAATGCCAAGGCAGATTCTA GAGGAAGAAGTCGAACAAAAATGGTGTCTCAATCACAGCCTGGTAGCCGGTCTGGGTCTCCAGGAAGAGT TCTGACCACAACAGCCCTGTCCACTGTGAGCTCTGGTGTTCAAAGAGTCCTGGTCAATTCAGCCTCAGCA CAAAAAAGAAGCAAGATACCACGGAGCCAGGGCTGTAGCAGAGAGGCTAGTCCATCTAGGCTTTCAGTGG CCCGAAGCAGTCGTATTCCTCGACCAAGTGTGAGTCAAGGATGCAGCCGGGAAGCTAGTCGGGAGAGCAG CAGAGACACAAGTCCTGTTCGCTCTTTTCAGCCCCTCGCCTCCAGACACCATTCCAGATCAACTGGTGCC CTCTACGCCCCCGAAGTGTATGGGGCCTCAGGTCCAGGTTATGGGATCAGCCAATCAAGTCGACTGTCGT CTTCTGTTAGTGCCATGCGAGTCCTGAACACAGGTTCTGATGTGGAGGAGGCGGTGGCAGATGCCTTGAA AAAACCAGCTCGAAGAAGATATGAATCATATGGAATGCATTCAGATGATGACGCCAACAGCGATGCATCT AGTGCTTGTTCAGAACGCTCCTATAGTTCTCGAAATGGTAGTATTCCTACATATATGAGGCAGACGGAAG ATGTGGCAGAAGTCCTCAATAGATGTGCTAGTTCCAATTGGTCAGAAAGGAAAGAAGGCCTCCTAGGTCT GCAGAACTTATTAAAAAATCAGAGAACACTAAGTCGAGTTGAACTGAAAAGATTATGTGAAATTTTCACA AGAATGTTTGCTGACCCTCATGGCAAGAGAGTATTCAGCATGTTTTTGGAGACTCTAGTGGATTTCATAC AAGTCCACAAAGATGATCTTCAAGATTGGTTGTTTGTACTGCTGACACAACTACTAAAAAAAATGGGTGC TGATTTGCTTGGATCTGTTCAGGCAAAAGTTCAGAAAGCCCTTGATGTTACAAGAGAGTCTTTTCCAAAT GATCTTCAGTTCAATATTCTAATGAGATTTACAGTTGATCAGACCCAGACACCAAGCTTAAAGGTGAAGG TTGCTATCCTTAAATACATAGAAACTCTGGCCAAACAGATGGATCCAGGAGATTTTATAAATTCCAGTGA AACTCGCCTAGCAGTGTCTCGGGTCATCACTTGGACAACAGAACCCAAAAGTTCTGATGTTCGGAAGGCA GCACAGTCAGTGCTGATTTCATTATTTGAACTCAATACCCCAGAGTTTACAATGTTATTAGGAGCTTTAC CAAAAACTTTTCAGGATGGTGCTACCAAGCTTCTTCATAATCACCTTCGAAACACTGGCAATGGAACCCA GAGTTCCATGGGGAGTCCTTTGACAAGACCAACACCACGATCACCAGCTAACTGGTCCAGTCCTCTTACT TCTCCTACCAATACATCACAGAATACTTTATCTCCAAGTGCATTTGATTATGACACAGAAAATATGAACT CTGAAGATATTTATAGCTCTCTTAGAGGTGTCACTGAAGCAATCCAGAATTTCAGCTTCCGTAGCCAAGA AGATATGAATGAGCCATTGAAAAGGGATTCTAAAAAAGATGATGGCGATTCAATGTGTGGTGGTCCTGGG ATGTCTGACCCAAGAGCAGGAGGTGATGCTACTGACTCAAGTCAAACAGCTCTTGATAATAAAGCTTCAT TGCTCCATTCAATGCCTACTCACTCCTCTCCACGCTCTCGAGACTATAATCCATATAACTATTCAGATAG CATCAGTCCCTTCAACAAGTCTGCCCTCAAGGAAGCCATGTTTGATGATGATGCTGACCAGTTTCCTGAC GATCTTTCCCTAGATCATTCTGACCTAGTTGCAGAGTTGTTGAAGGAGCTGTCTAACCATAATGAGCGTG TAGAAGAAAGAAAAATTGCCCTCTATGAACTTATGAAACTGACACAGGAAGAATCTTTTAGTGTTTGGGA TGAACACTTCAAAACAATATTGCTTTTATTGCTTGAAACGCTTGGAGATAAAGAGCCTACAATCAGGGCT TTGGCATTAAAGGTTTTAAGAGAAATCCTAAGGCATCAACCAGCAAGATTTAAAAACTATGCAGAATTGA CTGTCATGAAAACATTGGAAGCACATAAAGATCCTCATAAGGAGGTGGTGAGATCTGCTGAGGAAGCGGC ATCAGTGTTGGCCACTTCAATTAGTCCAGAGCAGTGCATCAAAGTGCTTTGTCCTATCATTCAAACTGCA GACTACCCAATTAATCTGGCTGCAATCAAAATGCAAACAAAAGTGATAGAGAGAGTGTCCAAGGAAACCC TAAACCTGCTTTTGCCAGAGATTATGCCAGGTCTAATACAGGGTTATGATAATTCAGAGAGCAGTGTTCG GAAAGCTTGTGTCTTCTGCCTGGTGGCTGTTCATGCGGTAATTGGTGATGAACTAAAACCACATCTCAGT CAACTTACTGGCAGTAAAATGAAGCTACTGAATCTTTACATCAAACGTGCACAAACAGGTTCTGGAGGAG CTGATCCCACTACTGATGTTTCTGGACAAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233210 representing NM_001207044 Red=Cloning site Green=Tags(s) MAMGDDKSFDDEESVDGNRPSSAASAFKVPAPKTSGNPANSARKPGSAGGPKVGGASKEGGAGAVDEDDF IKAFTDVPSIQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIRSLLVAGAAQYDCFFQHLRLLDGA LKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFIIRHTHV PRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKGIHDADAEARVEARKTYMG LRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSSQESLNRPFSSKWSTANPSTVAGR VSAGSSKASSLPGSLQRSRSDIDVNAAAGAKAHHAAGQSVRSGRLGAGALNAGSYASLEDTSDKLDGTAS EDGRVRAKLSAPLAGMGNAKADSRGRSRTKMVSQSQPGSRSGSPGRVLTTTALSTVSSGVQRVLVNSASA QKRSKIPRSQGCSREASPSRLSVARSSRIPRPSVSQGCSREASRESSRDTSPVRSFQPLASRHHSRSTGA LYAPEVYGASGPGYGISQSSRLSSSVSAMRVLNTGSDVEEAVADALKKPARRRYESYGMHSDDDANSDAS SACSERSYSSRNGSIPTYMRQTEDVAEVLNRCASSNWSERKEGLLGLQNLLKNQRTLSRVELKRLCEIFT RMFADPHGKRVFSMFLETLVDFIQVHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRESFPN DLQFNILMRFTVDQTQTPSLKVKVAILKYIETLAKQMDPGDFINSSETRLAVSRVITWTTEPKSSDVRKA AQSVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPRSPANWSSPLT SPTNTSQNTLSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMNEPLKRDSKKDDGDSMCGGPG MSDPRAGGDATDSSQTALDNKASLLHSMPTHSSPRSRDYNPYNYSDSISPFNKSALKEAMFDDDADQFPD DLSLDHSDLVAELLKELSNHNERVEERKIALYELMKLTQEESFSVWDEHFKTILLLLLETLGDKEPTIRA LALKVLREILRHQPARFKNYAELTVMKTLEAHKDPHKEVVRSAEEAASVLATSISPEQCIKVLCPIIQTA DYPINLAAIKMQTKVIERVSKETLNLLLPEIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDELKPHLS QLTGSKMKLLNLYIKRAQTGSGGADPTTDVSGQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001207044
ORF Size	3882 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001207044.3
RefSeq Size	6733 bp
RefSeq ORF	3885 bp
Locus ID	23122
UniProt ID	O75122
MW	141.5 kDa
Gene Summary	Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules (PubMed:26003921). Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2 (PubMed:16824950). This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (PubMed:16866869, PubMed:16914514). Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.[UniProtKB/Swiss-Prot Function]

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