EIF1AD (NM_001242486) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	haponin; OBELIX
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233341 representing NM_001242486. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTCAGGCCACCAAGAGGAAGCATGTGGTGAAGGAGGTGCTAGGGGAGCACATAGTGCCCTCCAAC CAGCAGCAGATTGTCAGGGTACTCAGGACCCCAGGGAACAATCTGCATGAGGTGGAGACAGCCCAAGGG CAGCGCTTCCTGGTGAGCATGCCCTCCAAATACCGCAAGAACATCTGGATCAAGAGAGGGGACTTTCTC ATTGTTGACCCCATTGAAGAGGGAGAAAAGGTGAAGGCTGAAATCTCGTTTGTGCTCTGCAAGGACCAC GTGCGCTCTCTGCAGAAGGAGGGGTTTTGGCCTGAGGCCTTCTCTGAAGTGGCTGAGAAACACAACAAC AGGAACAGACAAACTCAACCAGAACTCCCAGCTGAGCCACAGTTATCAGGAGAGGAGTCCAGCTCAGAA GATGATTCTGACCTGTTTGTTAACACAAACCGCAGACAGTATCATGAGAGTGAGGAGGAGAGTGAAGAG GAGGAGGCAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC233341 Blue=ORF Red=Cloning site Green=Tag(s) MSQATKRKHVVKEVLGEHIVPSNQQQIVRVLRTPGNNLHEVETAQGQRFLVSMPSKYRKNIWIKRGDFL IVDPIEEGEKVKAEISFVLCKDHVRSLQKEGFWPEAFSEVAEKHNNRNRQTQPELPAEPQLSGEESSSE DDSDLFVNTNRRQYHESEEESEEEEAA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242486
ORF Size	495 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size	2696 bp
RefSeq ORF	498 bp
Locus ID	84285
UniProt ID	Q8N9N8
MW	19.1 kDa
Gene Summary	Plays a role into cellular response to oxidative stress. Decreases cell proliferation.[UniProtKB/Swiss-Prot Function]

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