C6orf134 (ATAT1) (NM_001254952) Human Tagged ORF Clone
CAT#: RC233712
- TrueORF®
ATAT1 (Myc-DDK tagged) - Homo sapiens alpha tubulin acetyltransferase 1 (ATAT1), transcript variant 5
ORF Plasmid: tGFP
"NM_001254952" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | alpha-TAT; alpha-TAT1; C6orf134; MEC17; Nbla00487; TAT |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC233712 representing NM_001254952
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTCCCGTTCGATGTGGACGCGCTGTTCCCGGAGCGGATCACGGTGCTGGACCAGCACCTGAGGC CCCCAGCCCGCCGACCCGGAACCACAACGCCGGCCCGTGTTGATCTACAGCAGCAAATTATGACCATTAT AGATGAACTGGGCAAGGCTTCTGCCAAGGCCCAGAATCTTTCCGCTCCTATCACTAGTGCATCAAGGATG CAGAGTAACCGCCATGTTGTTTATATTCTCAAAGACAGTTCAGCCCGACCGGCTGGAAAAGGAGCCATTA TTGGTTTCATCAAAGTTGGATACAAGAAGCTCTTTGTACTGGATGATCGTGAGGCTCATAATGAGGTAGA ACCACTTTGCATCCTGGACTTTTACATCCATGAGTCTGTGCAACGCCATGGCCATGGGCGAGAACTCTTC CAGTATATGTTGCAGAAGGAGCGAGTGGAACCGCACCAACTGGCAATTGACCGACCCTCACAGAAGCTGC TGAAATTCCTGAATAAGCACTACAATCTGGAGACCACAGTCCCACAGGTGAACAACTTTGTGATCTTTGA AGGCTTCTTTGCCCATCAACATCCTCCAGCAAGGAAGCTGCCACCCAAGAGAGCAGAGGGAGACATCAAG CCATACTCCTCTAGTGACCGAGAATTTCTGAAGGTAGCTGTGGAGCCTCCTTGGCCCCTAAACAGGGCCC CTCGCCGCGCCACACCTCCAGCCCACCCACCCCCCCGCTCCAGCAGCCTGGGAAACTCACCAGAACGAGG TCCCCTCCGCCCCTTTGTGCCAGAGCAGGAGCTGCTGCGTTCCTTGCGCCTCTGCCCCCCACACCCTACC GCCCGCCTTCTGTTGGCTGCTGACCCTGGGGGCAGCCCAGCTCAACGTCGTCGCACCAGCTCCCTTCCCC GCTCTGAGGAGAGTCGATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC233712 representing NM_001254952
Red=Cloning site Green=Tags(s) MEFPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNLSAPITSASRM QSNRHVVYILKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELF QYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHPPARKLPPKRAEGDIK PYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPT ARLLLAADPGGSPAQRRRTSSLPRSEESRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001254952 |
ORF Size | 930 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001254952.3 |
RefSeq Size | 2098 bp |
RefSeq ORF | 933 bp |
Locus ID | 79969 |
UniProt ID | Q5SQI0 |
MW | 35.6 kDa |
Gene Summary | This gene encodes a protein that localizes to clathrin-coated pits, where it acetylates alpha tubulin on lysine 40. This process may be important in microtubule growth, for instance during chemotaxis and the formation of cilium. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
Documents
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Resources
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