DEDD2 (NM_001270614) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	FLAME-3; FLAME3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC233773 representing NM_001270614 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTATCCGGGTCGACCCCGGCCCCGTGCTGGGAGGAGGATGAGTGCCTGGACTACTACGGGATGC TGTCGCTTCACCGTATGTTCGAGGTGGTGGGCGGGCAACTGACCGAGTGCGAGCTGGAGCTCCTGGCCTT TCTGCTGGATGAGGCTCCTGGCGCCGCCGGAGGCTTAGCCCGGGCCCGCAGCGGCCTAGAGCTCCTGCTG GAGCTGGAGCGCCGCGGGCAGTGCGACGAGAGCAACCTGCGGCTGCTGGGGCAACTCCTGCGCGTGCTGG CCCGCCACGACCTGCTGCCGCACCTGGCGCGCAAGCGGCGCCGGCCAGTGTCTCCAGAACGCTATAGCTA TGGCACCTCCAGCTCTTCAAAGAGGACAGAGGGTAGCTGCCGTCGCCGTCGGCAGTCAAGCAGTTCTGCA AATTCTCAGCAGGGTCAGTGGGAGACAGGCTCCCCCCCAACCAAGCGGCAGCGGCGGAGTCGGGGCCGGC CCAGTGGTGGTGCCAGACGGCGGCGGAGAGGGGCCCCAGCCGCACCCCAGCAGCAGTCAGAGCCCGCCAG ACCTTCCTCTGAAGGCAAAGTGACCTGTGACATCCGGCTCCGGGTTCGAGCAGAGTACTGCGAGCATGGG CCAGCCTTGGAGCAGGGCGTGGCATCCCGGCGGCCCCAGGCGCTGGCGCGGCAGCTGGACGTGTTTGGGC AGGCCACCGCAGTGCTGCGCTCAAGGGACCTGGGCTCTGTGGTTTGTGACATCAAGTTCTCAGAGCTCTC CTATCTGGACGCCTTCTGGGGCGACTACCTGAGTGGCGCCCTGCTGCAGGCCCTGCGGGGCGTGTTCCTG ACTGAGGCCCTGCGAGAGGCTGTGGGCCGGGAGGCTGTTCGCCTGCTGGTCAGTGTGGATGAGGCTGACT ATGAGGCTGGCCGGCGCCGCCTGTTGCTGATGGAGGAGGAAGGGGGGCGGCGCCCGACAGAGGCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC233773 representing NM_001270614 Red=Cloning site Green=Tags(s) MALSGSTPAPCWEEDECLDYYGMLSLHRMFEVVGGQLTECELELLAFLLDEAPGAAGGLARARSGLELLL ELERRGQCDESNLRLLGQLLRVLARHDLLPHLARKRRRPVSPERYSYGTSSSSKRTEGSCRRRRQSSSSA NSQQGQWETGSPPTKRQRRSRGRPSGGARRRRRGAPAAPQQQSEPARPSSEGKVTCDIRLRVRAEYCEHG PALEQGVASRRPQALARQLDVFGQATAVLRSRDLGSVVCDIKFSELSYLDAFWGDYLSGALLQALRGVFL TEALREAVGREAVRLLVSVDEADYEAGRRRLLLMEEEGGRRPTEAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001270614
ORF Size	978 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270614.2
RefSeq Size	1943 bp
RefSeq ORF	981 bp
Locus ID	162989
UniProt ID	Q8WXF8
Protein Families	Druggable Genome, Transcription Factors
MW	36.6 kDa
Gene Summary	This gene encodes a nuclear-localized protein containing a death effector domain (DED). The encoded protein may regulate the trafficking of caspases and other proteins into the nucleus during death receptor-induced apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

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