ORP1 (OSBPL1A) (NM_001242508) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ORP-1; ORP1; OSBPL1B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234454 representing NM_001242508 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTAAGGAGTGTGACATGGCTAAAGAAATGCTTCCATCATTTCTTCAGAAAGTTGAAGTTGTCTCAG AAGCTTCTAGAGAAACTTGTGTAGCTTTGACTGATTGCCTTAATCTCTTCACCAAACAAGAAGGGGTGAG GAATTTTAAATTGGAACAAGAGCAAGAAAAAAACAAAATCTTGTCAGAAGCACTGGAGACGCTGGCCACT GAACATCATGAATTAGAGCAGTCTCTGGTGAAAGGCTCTCCACCCGCCAGCATCCTTAGCGAGGACGAGT TCTATGATGCGCTGTCAGATTCCGAGTCCGAAAGGTCCCTGAGTAGATTGGAAGCAGTGACAGCACGCTC CTTTGAAGAGGAAGGAGAGCATTTGGGCAGTAGAAAACACAGAATGTCCGAAGAAAAAGACTGTGGTGGC GGAGATGCTCTCTCCAATGGCATCAAGAAACACAGAACAAGTTTGCCTTCTCCTATGTTTTCCAGAAATG ACTTCAGTATCTGGAGCATCCTCAGAAAATGTATTGGAATGGAACTATCCAAGATCACGATGCCAGTTAT ATTTAATGAGCCTCTGAGCTTCCTACAGCGCCTAACTGAATACATGGAGCATACTTACCTCATCCACAAG GCCAGTTCACTCTCTGATCCTGTGGAAAGGATGCAGTGTGTAGCTGCGTTTGCTGTATCTGCTGTTGCTT CTCAGTGGGAACGGACTGGAAAACCTTTCAACCCACTGCTGGGAGAGACTTATGAATTAGTGCGAGATGA CCTTGGATTTAGACTCATCTCCGAACAGGTCAGCCATCACCCACCAATCAGTGCATTTCATGCTGAAGGA TTAAACAATGACTTCATCTTTCATGGCTCTATCTATCCCAAACTGAAATTCTGGGGGAAGAGTGTAGAAG CAGAACCCAAAGGAACCATCACCTTGGAGCTCCTTGAACACAATGAGGCATATACATGGACAAATCCCAC CTGCTGTGTGCATAATATCATTGTGGGTAAACTGTGGATCGAACAGTATGGCAATGTGGAAATTATAAAC CACAAGACTGGGGACAAATGTGTGTTGAATTTTAAGCCATGTGGCCTTTTTGGTAAGGAATTACACAAAG TTGAAGGCTACATTCAAGATAAAAGCAAAAAGAAGCTCTGTGCCCTCTATGGGAAGTGGACTGAATGTTT ATACAGTGTTGACCCTGCCACGTTTGACGCTTACAAAAAAAATGATAAGAAAAATACAGAAGAGAAGAAG AACAGCAAACAGATGAGCACCTCTGAGGAGTTGGATGAAATGCCAGTGCCGGATTCTGAAAGTGTATTCA TTATCCCTGGAAGCGTTCTTCTATGGCGAATAGCCCCACGGCCTCCAAATTCTGCCCAGATGTATAATTT TACTAGTTTTGCAATGGTTTTGAATGAAGTAGACAAAGACATGGAGAGTGTGATTCCCAAGACAGACTGC AGGTTACGGCCTGACATCAGAGCCATGGAAAATGGAGAGATAGATCAAGCTAGTGAAGAAAAAAAACGAC TTGAGGAAAAACAAAGAGCAGCCCGCAAAAACAGGTCCAAGTCAGAAGAGGACTGGAAGACGAGGTGGTT CCATCAAGGTCCTAATCCCTACAATGGAGCACAGGACTGGATTTACTCTGGCAGCTACTGGGACAGAAAT TACTTCAATTTGCCTGACATTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234454 representing NM_001242508 Red=Cloning site Green=Tags(s) MIKECDMAKEMLPSFLQKVEVVSEASRETCVALTDCLNLFTKQEGVRNFKLEQEQEKNKILSEALETLAT EHHELEQSLVKGSPPASILSEDEFYDALSDSESERSLSRLEAVTARSFEEEGEHLGSRKHRMSEEKDCGG GDALSNGIKKHRTSLPSPMFSRNDFSIWSILRKCIGMELSKITMPVIFNEPLSFLQRLTEYMEHTYLIHK ASSLSDPVERMQCVAAFAVSAVASQWERTGKPFNPLLGETYELVRDDLGFRLISEQVSHHPPISAFHAEG LNNDFIFHGSIYPKLKFWGKSVEAEPKGTITLELLEHNEAYTWTNPTCCVHNIIVGKLWIEQYGNVEIIN HKTGDKCVLNFKPCGLFGKELHKVEGYIQDKSKKKLCALYGKWTECLYSVDPATFDAYKKNDKKNTEEKK NSKQMSTSEELDEMPVPDSESVFIIPGSVLLWRIAPRPPNSAQMYNFTSFAMVLNEVDKDMESVIPKTDC RLRPDIRAMENGEIDQASEEKKRLEEKQRAARKNRSKSEEDWKTRWFHQGPNPYNGAQDWIYSGSYWDRN YFNLPDIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242508
ORF Size	1704 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242508.1, NP_001229437.1
RefSeq Size	2968 bp
RefSeq ORF	1707 bp
Locus ID	114876
UniProt ID	Q9BXW6
MW	65.5 kDa
Gene Summary	This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq, Jul 2008]

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