ORP1 (OSBPL1A) (NM_001242508) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ORP-1; ORP1; OSBPL1B
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331820 representing NM_001242508. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATTAAGGAGTGTGACATGGCTAAAGAAATGCTTCCATCATTTCTTCAGAAAGTTGAAGTTGTCTCA GAAGCTTCTAGAGAAACTTGTGTAGCTTTGACTGATTGCCTTAATCTCTTCACCAAACAAGAAGGGGTG AGGAATTTTAAATTGGAACAAGAGCAAGAAAAAAACAAAATCTTGTCAGAAGCACTGGAGACGCTGGCC ACTGAACATCATGAATTAGAGCAGTCTCTGGTGAAAGGCTCTCCACCCGCCAGCATCCTTAGCGAGGAC GAGTTCTATGATGCGCTGTCAGATTCCGAGTCCGAAAGGTCCCTGAGTAGATTGGAAGCAGTGACAGCA CGCTCCTTTGAAGAGGAAGGAGAGCATTTGGGCAGTAGAAAACACAGAATGTCCGAAGAAAAAGACTGT GGTGGCGGAGATGCTCTCTCCAATGGCATCAAGAAACACAGAACAAGTTTGCCTTCTCCTATGTTTTCC AGAAATGACTTCAGTATCTGGAGCATCCTCAGAAAATGTATTGGAATGGAACTATCCAAGATCACGATG CCAGTTATATTTAATGAGCCTCTGAGCTTCCTACAGCGCCTAACTGAATACATGGAGCATACTTACCTC ATCCACAAGGCCAGTTCACTCTCTGATCCTGTGGAAAGGATGCAGTGTGTAGCTGCGTTTGCTGTATCT GCTGTTGCTTCTCAGTGGGAACGGACTGGAAAACCTTTCAACCCACTGCTGGGAGAGACTTATGAATTA GTGCGAGATGACCTTGGATTTAGACTCATCTCCGAACAGGTCAGCCATCACCCACCAATCAGTGCATTT CATGCTGAAGGATTAAACAATGACTTCATCTTTCATGGCTCTATCTATCCCAAACTGAAATTCTGGGGG AAGAGTGTAGAAGCAGAACCCAAAGGAACCATCACCTTGGAGCTCCTTGAACACAATGAGGCATATACA TGGACAAATCCCACCTGCTGTGTGCATAATATCATTGTGGGTAAACTGTGGATCGAACAGTATGGCAAT GTGGAAATTATAAACCACAAGACTGGGGACAAATGTGTGTTGAATTTTAAGCCATGTGGCCTTTTTGGT AAGGAATTACACAAAGTTGAAGGCTACATTCAAGATAAAAGCAAAAAGAAGCTCTGTGCCCTCTATGGG AAGTGGACTGAATGTTTATACAGTGTTGACCCTGCCACGTTTGACGCTTACAAAAAAAATGATAAGAAA AATACAGAAGAGAAGAAGAACAGCAAACAGATGAGCACCTCTGAGGAGTTGGATGAAATGCCAGTGCCG GATTCTGAAAGTGTATTCATTATCCCTGGAAGCGTTCTTCTATGGCGAATAGCCCCACGGCCTCCAAAT TCTGCCCAGATGTATAATTTTACTAGTTTTGCAATGGTTTTGAATGAAGTAGACAAAGACATGGAGAGT GTGATTCCCAAGACAGACTGCAGGTTACGGCCTGACATCAGAGCCATGGAAAATGGAGAGATAGATCAA GCTAGTGAAGAAAAAAAACGACTTGAGGAAAAACAAAGAGCAGCCCGCAAAAACAGGTCCAAGTCAGAA GAGGACTGGAAGACGAGGTGGTTCCATCAAGGTCCTAATCCCTACAATGGAGCACAGGACTGGATTTAC TCTGGCAGCTACTGGGACAGAAATTACTTCAATTTGCCTGACATTTATTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242508
Insert Size	1707 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242508.1
RefSeq Size	2968 bp
RefSeq ORF	1707 bp
Locus ID	114876
UniProt ID	Q9BXW6
MW	65.1 kDa
Gene Summary	This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3, also known as OSBPL1C) lacks multiple exons from the 5' end and has an alternate 5' exon, which results in a downstream AUG start codon, compared to variant 2. The resulting isoform (3, also known as C) has a shorter N-terminus, and it lacks ankyrin repeats and the pleckstrin homology domain, but contains the sterol-binding domain, compared to isoform 2.

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