SRP68 (NM_001260502) Human Tagged ORF Clone
CAT#: RC234506
- TrueORF®
SRP68 (Myc-DDK tagged) - Homo sapiens signal recognition particle 68kDa (SRP68), transcript variant 2
ORF Plasmid: tGFP
"NM_001260502" in other vectors (2)
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CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234506 representing NM_001260502
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTGAGAAGCAGGTCCCAGGCGGCGGCGGCGGCGGCGGCAGTGGCGGCGGCGGTGGCAGTGGCG GCGGCGGTAGCGGCGGTGGACGTGGTGCCGGAGGGGAAGAAAATAAAGAAAACGAACGCCCTTCGGCCGG ATCGAAGGCAAACAAAGAATTTGGGGATAGCCTGAGTTTGGAGATTCTTCAGATTATTAAGGAATCCCAG CAGCAGCATGGTTTACGGCATGGAGATTTTCAGAGGTACAGATACTTGCTTCTGGTTCTGATGGATGCTG AAAGAGCCTGGAGCTACGCCATGCAGCTGAAACAGGAAGCCAACACTGAACCCCGAAAACGGTTTCACTT GTTATCTCGCCTACGCAAAGCCGTGAAGCATGCAGAGGAATTGGAACGCTTGTGTGAGAGCAATCGCGTG GATGCCAAGACCAAATTAGAGGCTCAGGCTTACACAGCTTACCTCTCAGGAATGCTACGTTTTGAACATC AAGAATGGAAAGCTGCCATTGAGGCTTTTAACAAATGCAAAACTATCTATGAGAAGCTAGCCAGTGCTTT CACAGAGGAGCAGGCTGTGCTGTATAACCAACGTGTGGAAGAGATTTCACCCAACATCCGCTATTGTGCA TATAATATTGGGGACCAGTCAGCCATCAATGAACTCATGCAGATGAGATTGAGGTCTGGGGGCACTGAGG GTCTCTTGGCTGAAAAATTGGAGGCTTTGATCACTCAGACTCGAGCCAAACAGGCAGCTACCATGAGTGA AGTGGAGTGGAGAGGGAGAACGGTTCCAGTGAAGATTGACAAAGTGCGCATTTTCTTATTAGGACTGGCT GATAACGAAGCAGCTATTGTCCAGGCTGAAAGCGAAGAAACTAAGGAGCGCCTGTTTGAATCAATGCTCA GCGAGTGTCGGGACGCCATCCAGGTGGTTCGGGAGGAGCTCAAGCCAGATCAGAAACAGAGAGATTATAT CCTTGAAGGAGAGCCAGGGAAGGTGTCTAATCTTCAATACTTGCATAGCTACCTGACTTACATCAAGCTA TCAACGGCAATCAAGCGTAATGAGAACATGGCCAAAGGTCTGCAGAGGGCTCTGCTGCAGCAGCAGCCAG AGGATGACAGCAAGCGCTCACCCCGGCCCCAGGACCTGATCCGACTCTATGACATCATCTTACAGAATCT GGTGGAATTGCTCCAGCTTCCTGGTTTAGAGGAAGACAAAGCCTTCCAGAAAGAGATAGGCCTCAAGACT CTGGTGTTCAAAGCTTACAGGTGTTTTTTCATTGCTCAGTCCTATGTGCTGGTGAAGAAGTGGAGCGAAG CCCTTGTCCTGTATGACAGAGTCCTGAAATATGCAAATGAAGTAAATTCTGATGCTGGCGCCTTCAAGAA CAGCCTAAAGGACCTGCCTGATGTGCAAGAGCTCATCACTCAAGTGCGGTCAGAGAAGTGCTCCCTGCAG GCCGCAGCCATCCTTGATGCAAACGACGCTCATCAAACAGAGACCTCCTCCTCCCAAGTCAAGGACAATA AGCCTCTGGTTGAACGGTTTGAGACATTCTGCCTGGACCCTTCCCTTGTCACCAAGCAAGCCAACCTTGT GCACTTCCCACCAGGCTTCCAGCCCATTCCCTGCAAGCCTTTGTTCTTTGACCTGGCCCTCAACCATGTG GCTTTCCCACCCCTTGAGGACAAGTTGGAACAGAAGACCAAGAGTGGCCTCACTGGATACATCAAGGGCA TCTTTGGATTCAGGAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234506 representing NM_001260502
Red=Cloning site Green=Tags(s) MAAEKQVPGGGGGGGSGGGGGSGGGGSGGGRGAGGEENKENERPSAGSKANKEFGDSLSLEILQIIKESQ QQHGLRHGDFQRYRYLLLVLMDAERAWSYAMQLKQEANTEPRKRFHLLSRLRKAVKHAEELERLCESNRV DAKTKLEAQAYTAYLSGMLRFEHQEWKAAIEAFNKCKTIYEKLASAFTEEQAVLYNQRVEEISPNIRYCA YNIGDQSAINELMQMRLRSGGTEGLLAEKLEALITQTRAKQAATMSEVEWRGRTVPVKIDKVRIFLLGLA DNEAAIVQAESEETKERLFESMLSECRDAIQVVREELKPDQKQRDYILEGEPGKVSNLQYLHSYLTYIKL STAIKRNENMAKGLQRALLQQQPEDDSKRSPRPQDLIRLYDIILQNLVELLQLPGLEEDKAFQKEIGLKT LVFKAYRCFFIAQSYVLVKKWSEALVLYDRVLKYANEVNSDAGAFKNSLKDLPDVQELITQVRSEKCSLQ AAAILDANDAHQTETSSSQVKDNKPLVERFETFCLDPSLVTKQANLVHFPPGFQPIPCKPLFFDLALNHV AFPPLEDKLEQKTKSGLTGYIKGIFGFRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001260502 |
ORF Size | 1767 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001260502.1, NP_001247431.1 |
RefSeq Size | 2736 bp |
RefSeq ORF | 1770 bp |
Locus ID | 6730 |
UniProt ID | Q9UHB9 |
Protein Pathways | Protein export |
MW | 66.6 kDa |
Gene Summary | This gene encodes a subunit of the signal recognition particle (SRP). The SRP is a ribonucleoprotein complex that transports secreted and membrane proteins to the endoplasmic reticulum for processing. The complex includes a 7S RNA and six protein subunits. The encoded protein is the 68kDa component of the SRP, and forms a heterodimer with the 72kDa subunit that is required for SRP function. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and three pseudogenes of this gene are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, May 2012] |
Documents
Product Manuals |
FAQs |
SDS |