CHD1L (NM_001256337) Human Tagged ORF Clone
CAT#: RC234572
- TrueORF®
CHD1L (Myc-DDK tagged) - Homo sapiens chromodomain helicase DNA binding protein 1-like (CHD1L), transcript variant 4
ORF Plasmid: tGFP
"NM_001256337" in other vectors (2)
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CNY 6,180.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ALC1; CHDL |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234572 representing NM_001256337
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGCATTGCAGAAGAAATACTACAAGGCCATTTTGATGAAAGACCTAGATGCATTTGAAAATGAGA CGGCAAAGAAAGTTAAACTACAGAACATTTTGTCCCAGCTTCGAAAGTGTGTGGATCACCCATATTTGTT TGATGGTGTGGAGCCGGAGCCTTTTGAAGTTGGAGACCACCTGACTGAGGCTAGTGGGAAGCTTCACCTG CTGGATAAGCTACTAGCATTCCTGTATTCTGGGGGCCATCGGGTTTTACTTTTCTCCCAAATGACCCAGA TGTTGGATATTCTCCAAGACTATATGGATTACAGAGGCTACAGCTATGAGCGTGTGGATGGTTCTGTGAG AGGAGAAGAGAGACACTTGGCCATTAAGAACTTTGGACAGCAGCCCATTTTCGTTTTTCTCCTGAGTACT AGGGCAGGTGGAGTTGGCATGAACTTAACAGCAGCAGATACTGTGATTTTTGTTGACAGTGACTTTAATC CTCAGAATGACTTGCAAGCAGCTGCCAGGGCTCATCGCATTGGCCAAAACAAGTCTGTTAAAGTTATTCG GCTGATTGGTCGAGACACTGTGGAAGAAATAGTCTATAGGAAAGCAGCCTCCAAACTGCAGCTCACCAAC ATGATCATAGAAGGAGGCCATTTTACTCTGGGAGCCCAGAAACCCGCTGCCGATGCTGACCTCCAGTTGA GTGAGATACTCAAATTTGGTTTGGATAAACTGCTGGCCTCTGAGGGGAGCACCATGGATGAAATAGACCT GGAGTCCATCCTGGGAGAAACAAAAGATGGCCAGTGGGTCTCTGATGCCTTGCCTGCAGCAGAAGGAGGG AGCAGAGATCAAGAGGAAGGAAAAAATCATATGTACTTATTTGAAGGTAAAGATTATTCTAAAGAGCCCA GTAAGGAAGACAGAAAATCATTTGAACAACTGGTAAACCTTCAGAAAACCCTTTTGGAGAAAGCTAGTCA AGAGGGCCGATCACTCCGAAATAAAGGCAGTGTTCTCATCCCAGGCCTTGTGGAGGGATCTACCAAAAGG AAGCGGGTTCTGAGTCCAGAAGAGCTGGAGGACAGACAGAAGAAAAGACAAGAAGCAGCTGCCAAGAGAA GGAGACTCATAGAGGAGAAGAAGAGGCAAAAGGAAGAGGCTGAACATAAGAAAAAGATGGCCTGGTGGGA ATCCAACAATTACCAGTCCTTCTGCCTGCCCTCTGAGGAGAGCGAGCCAGAGGACCTTGAGAATGGGGAA GAGAGCTCTGCTGAGCTGGATTACCAAGACCCAGATGCTACTTCCCTCAAGTACGTTAGTGGTGATGTCA CCCACCCTCAGGCTGGGGCCGAGGATGCTCTCATTGTGCACTGCGTAGATGACTCTGGCCACTGGGGCAG AGGTGGTTTATTTACAGCTCTGGAAAAGCGATCCGCTGAGCCAAGAAAAATATATGAGCTGGCTGGGAAA ATGAAAGACCTGAGTTTGGGAGGTGTCCTTTTATTTCCTGTTGATGATAAAGAATCAAGAAACAAAGGGC AAGATTTGTTGGCCTTGATTGTGGCTCAGCATCGTGATCGTTCCAATGTCCTGTCTGGCATTAAGATGGC AGCCCTAGAAGAGGGCCTGAAGAAGATATTTTTAGCAGCAAAAAAGAAGAAAGCAAGTGTTCATCTTCCA CGTATTGGACATGCCACGAAAGGTTTTAACTGGTATGGTACTGAGCGACTTATTCGGAAACATCTGGCTG CAAGAGGCATCCCAACTTACATATATTATTTTCCTAGAAGCAAGTCTGCTGTCCTTCATTCACAGTCTTC ATCTTCCTCCTCAAGACAGCTGGTGCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234572 representing NM_001256337
Red=Cloning site Green=Tags(s) MSALQKKYYKAILMKDLDAFENETAKKVKLQNILSQLRKCVDHPYLFDGVEPEPFEVGDHLTEASGKLHL LDKLLAFLYSGGHRVLLFSQMTQMLDILQDYMDYRGYSYERVDGSVRGEERHLAIKNFGQQPIFVFLLST RAGGVGMNLTAADTVIFVDSDFNPQNDLQAAARAHRIGQNKSVKVIRLIGRDTVEEIVYRKAASKLQLTN MIIEGGHFTLGAQKPAADADLQLSEILKFGLDKLLASEGSTMDEIDLESILGETKDGQWVSDALPAAEGG SRDQEEGKNHMYLFEGKDYSKEPSKEDRKSFEQLVNLQKTLLEKASQEGRSLRNKGSVLIPGLVEGSTKR KRVLSPEELEDRQKKRQEAAAKRRRLIEEKKRQKEEAEHKKKMAWWESNNYQSFCLPSEESEPEDLENGE ESSAELDYQDPDATSLKYVSGDVTHPQAGAEDALIVHCVDDSGHWGRGGLFTALEKRSAEPRKIYELAGK MKDLSLGGVLLFPVDDKESRNKGQDLLALIVAQHRDRSNVLSGIKMAALEEGLKKIFLAAKKKKASVHLP RIGHATKGFNWYGTERLIRKHLAARGIPTYIYYFPRSKSAVLHSQSSSSSSRQLVP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001256337 |
ORF Size | 1848 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001256337.3 |
RefSeq Size | 2506 bp |
RefSeq ORF | 1851 bp |
Locus ID | 9557 |
UniProt ID | Q86WJ1 |
MW | 69.6 kDa |
Gene Summary | This gene encodes a DNA helicase protein involved in DNA repair. The protein converts ATP to add poly(ADP-ribose) as it regulates chromatin relaxation following DNA damage. Overexpression of this gene has been linked to several types of cancers. [provided by RefSeq, Feb 2017] |
Documents
Product Manuals |
FAQs |
SDS |