TRAK1 (NM_001265609) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	EIEE68; MILT1; OIP106
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234686 representing NM_001265609 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAAATTTATCGAAGCGGATTATTATGAACTAGACTGGTATTATGAAGAATGCTCGGATGTTTTAT GTGCTGAAAGAGTTGGCCAGATGACTAAGACATATAATGACATAGATGCTGTCACTCGGCTTCTTGAGGA GAAAGAGCGGGATTTAGAATTGGCCGCTCGCATCGGCCAGTCGTTGTTGAAGAAGAACAAGACCCTAACC GAGAGGAACGAGCTGCTGGAGGAGCAGGTGGAACACATCAGGGAGGAGGTGTCTCAGCTCCGGCATGAGC TGTCCATGAAGGATGAGCTGCTTCAGTTCTACACCAGCGCTGCGGAGGAGAGTGAGCCCGAGTCCGTTTG CTCAACCCCGTTGAAGAGGAATGAGTCGTCCTCCTCAGTCCAGAATTACTTTCATTTGGATTCTCTTCAA AAGAAGCTGAAAGACCTTGAAGAGGAGAATGTTGTACTTCGATCCGAGGCCAGCCAGCTGAAGACAGAGA CCATCACCTATGAGGAGAAGGAGCAGCAGCTGGTCAATGACTGCGTGAAGGAGCTGAGGGATGCCAATGT CCAGATTGCTAGTATCTCAGAGGAACTGGCCAAGAAGACGGAAGATGCTGCCCGCCAGCAAGAGGAGATC ACACACCTGCTATCGCAAATAGTTGATTTGCAGAAAAAGGCAAAAGCTTGCGCAGTGGAAAATGAAGAAC TTGTCCAGCATCTGGGGGCTGCTAAGGATGCCCAGCGGCAGCTCACAGCCGAGCTGCGTGAGCTGGAGGA CAAGTACGCAGAGTGCATGGAGATGCTGCATGAGGCGCAGGAGGAGCTGAAGAACCTCCGGAACAAAACC ATGCCCAATACCACGTCTCGGCGCTACCACTCACTGGGCCTGTTTCCCATGGATTCCTTGGCAGCAGAGA TTGAGGGAACGATGCGCAAGGAGCTGCAGTTGGAAGAGGCCGAGTCTCCAGACATCACTCACCAGAAGCG TGTCTTTGAGACAGTAAGAAACATCAACCAGGTTGTCAAGCAGAGATCTCTGACCCCTTCTCCCATGAAC ATCCCCGGCTCCAACCAGTCCTCGGCCATGAACTCCCTCCTGTCCAGCTGCGTCAGCACCCCCCGGTCCA GCTTCTACGGCAGCGACATAGGCAACGTCGTCCTCGACAACAAGACCAACAGCATCATTCTGGAAACAGA GGCAGCCGACCTGGGAAACGATGAGCGGAGTAAGAAGCCGGGGACGCCGGGCACCCCAGGCTCCCACGAC CTGGAGACGGCGCTGAGGCGGCTGTCCCTGCGCCGGGAGAACTACCTCTCGGAGAGGAGGTTCTTTGAGG AGGAGCAAGAGAGGAAGCTCCAGGAGCTGGCGGAGAAGGGCGAGCTGCGCAGCGGCTCCCTCACACCCAC TGAGAGCATCATGTCCCTGGGCACGCACTCCCGCTTCTCCGAGTTCACCGGCTTCTCTGGCATGTCCTTC AGCAGCCGCTCCTACCTGCCTGAGAAGCTCCAGATCGTGAAGCCGCTGGAAGGTTCCGCCACACTTCACC ACTGGCAGCAGTTGGCCCAACCTCACCTTGGGGGCATCCTGGACCCCCGGCCCGGTGTGGTCACCAAGGG CTTCCGGACGCTGGATGTTGACCTGGACGAAGTGTACTGCCTTAACGACTTTGAAGAAGATGACACAGGT GACCACATTTCTCTCCCACGCCTAGCTACCTCCACTCCAGTTCAGCACCCAGAGACCTCAGGTGAGAGGT CCCAAGCACGTGTGACTGTCTCAGGCAGCAGAAGTTACCCGAGCCGGCCTCAGGCTTCCCCAGAGGAGAT GCAGGAGCCGCCAGCGGCCACGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGGGTCTGGTGAGGGCACC ACGATAAGTCCTGTAAACTTGGCACCTTTCCCGGAGGCAGAGTTTTGGGCCATTCTCACCTCTGTTCCAG GCACCATCCGTAGTGGTTCTCTGTCTGTAGCTTCCGCTCGTCTGTGTGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234686 representing NM_001265609 Red=Cloning site Green=Tags(s) MQKFIEADYYELDWYYEECSDVLCAERVGQMTKTYNDIDAVTRLLEEKERDLELAARIGQSLLKKNKTLT ERNELLEEQVEHIREEVSQLRHELSMKDELLQFYTSAAEESEPESVCSTPLKRNESSSSVQNYFHLDSLQ KKLKDLEEENVVLRSEASQLKTETITYEEKEQQLVNDCVKELRDANVQIASISEELAKKTEDAARQQEEI THLLSQIVDLQKKAKACAVENEELVQHLGAAKDAQRQLTAELRELEDKYAECMEMLHEAQEELKNLRNKT MPNTTSRRYHSLGLFPMDSLAAEIEGTMRKELQLEEAESPDITHQKRVFETVRNINQVVKQRSLTPSPMN IPGSNQSSAMNSLLSSCVSTPRSSFYGSDIGNVVLDNKTNSIILETEAADLGNDERSKKPGTPGTPGSHD LETALRRLSLRRENYLSERRFFEEEQERKLQELAEKGELRSGSLTPTESIMSLGTHSRFSEFTGFSGMSF SSRSYLPEKLQIVKPLEGSATLHHWQQLAQPHLGGILDPRPGVVTKGFRTLDVDLDEVYCLNDFEEDDTG DHISLPRLATSTPVQHPETSGERSQARVTVSGSRSYPSRPQASPEEMQEPPAATEEEEEEEEEEGSGEGT TISPVNLAPFPEAEFWAILTSVPGTIRSGSLSVASARLCG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001265609
ORF Size	2010 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001265609.1, NP_001252538.1
RefSeq Size	4683 bp
RefSeq ORF	2013 bp
Locus ID	22906
Protein Families	Transcription Factors
MW	75.9 kDa
Gene Summary	Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors (PubMed:18675823). Involved in mitochondrial motility. When O-glycosylated, abolishes mitochondrial motility. Crucial for recruiting OGT to the mitochondrial surface of neuronal processes (PubMed:24995978). TRAK1 and RHOT form an essential protein complex that links KIF5 to mitochondria for light chain-independent, anterograde transport of mitochondria (By similarity).[UniProtKB/Swiss-Prot Function]

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