TRAK1 (NM_001265609) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EIEE68; MILT1; OIP106
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332823 representing NM_001265609. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCAGAAATTTATCGAAGCGGATTATTATGAACTAGACTGGTATTATGAAGAATGCTCGGATGTTTTA TGTGCTGAAAGAGTTGGCCAGATGACTAAGACATATAATGACATAGATGCTGTCACTCGGCTTCTTGAG GAGAAAGAGCGGGATTTAGAATTGGCCGCTCGCATCGGCCAGTCGTTGTTGAAGAAGAACAAGACCCTA ACCGAGAGGAACGAGCTGCTGGAGGAGCAGGTGGAACACATCAGGGAGGAGGTGTCTCAGCTCCGGCAT GAGCTGTCCATGAAGGATGAGCTGCTTCAGTTCTACACCAGCGCTGCGGAGGAGAGTGAGCCCGAGTCC GTTTGCTCAACCCCGTTGAAGAGGAATGAGTCGTCCTCCTCAGTCCAGAATTACTTTCATTTGGATTCT CTTCAAAAGAAGCTGAAAGACCTTGAAGAGGAGAATGTTGTACTTCGATCCGAGGCCAGCCAGCTGAAG ACAGAGACCATCACCTATGAGGAGAAGGAGCAGCAGCTGGTCAATGACTGCGTGAAGGAGCTGAGGGAT GCCAATGTCCAGATTGCTAGTATCTCAGAGGAACTGGCCAAGAAGACGGAAGATGCTGCCCGCCAGCAA GAGGAGATCACACACCTGCTATCGCAAATAGTTGATTTGCAGAAAAAGGCAAAAGCTTGCGCAGTGGAA AATGAAGAACTTGTCCAGCATCTGGGGGCTGCTAAGGATGCCCAGCGGCAGCTCACAGCCGAGCTGCGT GAGCTGGAGGACAAGTACGCAGAGTGCATGGAGATGCTGCATGAGGCGCAGGAGGAGCTGAAGAACCTC CGGAACAAAACCATGCCCAATACCACGTCTCGGCGCTACCACTCACTGGGCCTGTTTCCCATGGATTCC TTGGCAGCAGAGATTGAGGGAACGATGCGCAAGGAGCTGCAGTTGGAAGAGGCCGAGTCTCCAGACATC ACTCACCAGAAGCGTGTCTTTGAGACAGTAAGAAACATCAACCAGGTTGTCAAGCAGAGATCTCTGACC CCTTCTCCCATGAACATCCCCGGCTCCAACCAGTCCTCGGCCATGAACTCCCTCCTGTCCAGCTGCGTC AGCACCCCCCGGTCCAGCTTCTACGGCAGCGACATAGGCAACGTCGTCCTCGACAACAAGACCAACAGC ATCATTCTGGAAACAGAGGCAGCCGACCTGGGAAACGATGAGCGGAGTAAGAAGCCGGGGACGCCGGGC ACCCCAGGCTCCCACGACCTGGAGACGGCGCTGAGGCGGCTGTCCCTGCGCCGGGAGAACTACCTCTCG GAGAGGAGGTTCTTTGAGGAGGAGCAAGAGAGGAAGCTCCAGGAGCTGGCGGAGAAGGGCGAGCTGCGC AGCGGCTCCCTCACACCCACTGAGAGCATCATGTCCCTGGGCACGCACTCCCGCTTCTCCGAGTTCACC GGCTTCTCTGGCATGTCCTTCAGCAGCCGCTCCTACCTGCCTGAGAAGCTCCAGATCGTGAAGCCGCTG GAAGGTTCCGCCACACTTCACCACTGGCAGCAGTTGGCCCAACCTCACCTTGGGGGCATCCTGGACCCC CGGCCCGGTGTGGTCACCAAGGGCTTCCGGACGCTGGATGTTGACCTGGACGAAGTGTACTGCCTTAAC GACTTTGAAGAAGATGACACAGGTGACCACATTTCTCTCCCACGCCTAGCTACCTCCACTCCAGTTCAG CACCCAGAGACCTCAGGTGAGAGGTCCCAAGCACGTGTGACTGTCTCAGGCAGCAGAAGTTACCCGAGC CGGCCTCAGGCTTCCCCAGAGGAGATGCAGGAGCCGCCAGCGGCCACGGAGGAGGAGGAGGAGGAGGAG GAGGAGGAGGGGTCTGGTGAGGGCACCACGATAAGTCCTGTAAACTTGGCACCTTTCCCGGAGGCAGAG TTTTGGGCCATTCTCACCTCTGTTCCAGGCACCATCCGTAGTGGTTCTCTGTCTGTAGCTTCCGCTCGT CTGTGTGGGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001265609
Insert Size	2013 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001265609.1
RefSeq Size	4683 bp
RefSeq ORF	2013 bp
Locus ID	22906
Protein Families	Transcription Factors
MW	75.5 kDa
Gene Summary	Involved in the regulation of endosome-to-lysosome trafficking, including endocytic trafficking of EGF-EGFR complexes and GABA-A receptors (PubMed:18675823). Involved in mitochondrial motility. When O-glycosylated, abolishes mitochondrial motility. Crucial for recruiting OGT to the mitochondrial surface of neuronal processes (PubMed:24995978). TRAK1 and RHOT form an essential protein complex that links KIF5 to mitochondria for light chain-independent, anterograde transport of mitochondria (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' and 3' UTRs and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (4) is shorter and has distinct N- and C-termini, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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