TBC1D23 (NM_001199198) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NS4ATP1; PCH11
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234737 representing NM_001199198 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGGAGAAGATGTGCCGCCGCTGCCAACGTCGAGCGGCGACGGCTGGGAAAAAGATCTTGAAG AAGCTCTGGAAGCAGGAGGTTGTGATCTTGAAACGTTGAGAAATATAATTCAAGGAAGACCGCTGCCTGC TGATCTGAGGGCCAAAGTTTGGAAGATTGCTCTGAATGTTGCAGGAAAAGGTGATAGTTTGGCATCATGG GATGGTATTTTAGACTTGCCAGAACAGAACACTATTCACAAAGATTGCCTGCAGTTTATTGACCAGCTTT CAGTGCCAGAGGAGAAGGCAGCAGAATTACTTTTGGATATTGAATCTGTAATTACCTTTTATTGTAAATC ACGTAACATTAAATATAGCACATCCCTTAGCTGGATACATCTACTGAAACCATTGGTGCATCTTCAACTG CCACGCAGCGATTTATACAACTGCTTTTATGCCATAATGAATAAGTACATTCCCAGGGATTGTTCCCAGA AAGGGAGACCATTTCATCTCTTCAGGTTGCTCATCCAATACCATGAGCCTGAGCTTTGTTCTTATCTTGA TACAAAGAAAATTACTCCAGACTCCTATGCACTCAACTGGCTTGGAAGTCTTTTTGCATGTTACTGTTCC ACTGAAGTCACTCAGGCAATATGGGATGGATATCTACAACAAGCAGATCCATTTTTTATTTATTTCTTAA TGTTAATTATCCTTGTTAATGCAAAAGAAGTTATTTTAACACAAGAGTCAGACAGCAAAGAAGAAGTTAT CAAGTTCTTGGAAAATACTCCATCCAGTCTGAATATAGAAGATATAGAAGACCTTTTCTCTCTGGCTCAG TATTATTGCAGCAAAACACCGGCTTCTTTTAGGAAGGATAATCACCATCTCTTTGGTAGTACTTTGTTGG GAATTAAGGATGATGATGCAGATCTGAGTCAGGCTCTTTGTCTGGCCATCTCCGTGTCAGAGATCCTTCA AGCGAATCAGCTACAAGGGGAAGGAGTCCGGTTCTTTGTGGTGGATTGCCGTCCTGCAGAACAATATAAT GCTGGGCATTTATCAACTGCTTTCCACTTAGATTCAGACCTGATGCTTCAGAATCCATCTGAGTTTGCAC AGTCAGTAAAATCCTTGCTGGAAGCACAGAAGCAGTCCATTGAGTCTGGCTCCATAGCTGGTGGGGAGCA CCTCTGTTTTATGGGCAGTGGCAGGGAGGAAGAAGACATGTATATGAACATGGTCCTGGCACACTTTTTA CAGAAAAACAAAGAATATGTGAGTATTGCCAGTGGAGGATTTATGGCACTGCAGCAGCACCTGGCAGACA TTAATGTGGATGGACCAGAAAATGGATATGGCCATTGGATTGCTAGTACCTCAGGCTCAAGGAGCAGTAT CAATTCTGTTGATGGTGAATCTCCTAATGGCTCAAGTGATAGAGGAATGAAATCACTAGTAAATAAAATG ACTGTGGCTTTGAAGACAAAATCCGTTAATGTCAGGGAAAAAGTTATCAGTTTTATAGAGAATACATCAA CTCCTGTGGATCGAATGTCTTTCAATCTTCCTTGGCCAGACAGATCATGTACAGAGCGGCATGTGAGCAG CAGTGACAGAGTGGGCAAGCCTTACCGTGGCGTAAAGCCTGTTTTCAGCATTGGGGATGAAGAAGAATAC GACACAGATGAAATTGACAGTTCTTCAATGTCAGATGATGATAGAAAAGAGGTTGTAAACATTCAGACTT GGATAAACAAACCAGATGTCAAACATCATTTTCCTTGTAAAGAAGTAAAAGAAAGTGGACACATGTTTCC CAGTCATCTGTTGGTTACTGCAACACATATGTACTGTTTAAGGGAGATTGTTTCACGGAAAGGATTGGCT TATATACAGTCTCGACAAGCGCTGAATTCTGTAGTTAAAATTACATCCAAAAAAAAACATCCTGAACTCA TTACCTTCAAGTATGGAAATAGCAGTGCTTCAGGAATAGAAATCTTGGCAATCGAAAGGTATTTGATTCC AAATGCAGGGGATGCAACTAAAGCCATAAAACAGCAGATCATGAAAGTTTTGGATGCTTTGGAAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234737 representing NM_001199198 Red=Cloning site Green=Tags(s) MAEGEDVPPLPTSSGDGWEKDLEEALEAGGCDLETLRNIIQGRPLPADLRAKVWKIALNVAGKGDSLASW DGILDLPEQNTIHKDCLQFIDQLSVPEEKAAELLLDIESVITFYCKSRNIKYSTSLSWIHLLKPLVHLQL PRSDLYNCFYAIMNKYIPRDCSQKGRPFHLFRLLIQYHEPELCSYLDTKKITPDSYALNWLGSLFACYCS TEVTQAIWDGYLQQADPFFIYFLMLIILVNAKEVILTQESDSKEEVIKFLENTPSSLNIEDIEDLFSLAQ YYCSKTPASFRKDNHHLFGSTLLGIKDDDADLSQALCLAISVSEILQANQLQGEGVRFFVVDCRPAEQYN AGHLSTAFHLDSDLMLQNPSEFAQSVKSLLEAQKQSIESGSIAGGEHLCFMGSGREEEDMYMNMVLAHFL QKNKEYVSIASGGFMALQQHLADINVDGPENGYGHWIASTSGSRSSINSVDGESPNGSSDRGMKSLVNKM TVALKTKSVNVREKVISFIENTSTPVDRMSFNLPWPDRSCTERHVSSSDRVGKPYRGVKPVFSIGDEEEY DTDEIDSSSMSDDDRKEVVNIQTWINKPDVKHHFPCKEVKESGHMFPSHLLVTATHMYCLREIVSRKGLA YIQSRQALNSVVKITSKKKHPELITFKYGNSSASGIEILAIERYLIPNAGDATKAIKQQIMKVLDALES TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199198
ORF Size	2097 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199198.3
RefSeq Size	3886 bp
RefSeq ORF	2100 bp
Locus ID	55773
UniProt ID	Q9NUY8
Protein Families	Druggable Genome
MW	78.8 kDa
Gene Summary	Putative Rab GTPase-activating protein which plays a role in vesicular trafficking (PubMed:28823707). Involved in endosome-to-Golgi trafficking. Acts as a bridging protein by binding simultaneously to golgins, including GOLGA1 and GOLGA4, located at the trans-Golgi, and to the WASH complex, located on endosome-derived vesicles (PubMed:29084197, PubMed:29426865). Together with WDR11 complex facilitates the golgin-mediated capture of vesicles generated using AP-1 (PubMed:29426865). Plays a role in brain development, including in cortical neuron positioning (By similarity). May also be important for neurite outgrowth, possibly through its involvement in membrane trafficking and cargo delivery, 2 processes that are essential for axonal and dendritic growth (By similarity). May act as a general inhibitor of innate immunity signaling, strongly inhibiting multiple TLR and dectin/CLEC7A-signaling pathways. Does not alter initial activation events, but instead affects maintenance of inflammatory gene expression several hours after bacterial lipopolysaccharide (LPS) challenge (By similarity).[UniProtKB/Swiss-Prot Function]

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