TBC1D23 (NM_001199198) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NS4ATP1; PCH11
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331262 representing NM_001199198. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGAAGGAGAAGATGTGCCGCCGCTGCCAACGTCGAGCGGCGACGGCTGGGAAAAAGATCTTGAA GAAGCTCTGGAAGCAGGAGGTTGTGATCTTGAAACGTTGAGAAATATAATTCAAGGAAGACCGCTGCCT GCTGATCTGAGGGCCAAAGTTTGGAAGATTGCTCTGAATGTTGCAGGAAAAGGTGATAGTTTGGCATCA TGGGATGGTATTTTAGACTTGCCAGAACAGAACACTATTCACAAAGATTGCCTGCAGTTTATTGACCAG CTTTCAGTGCCAGAGGAGAAGGCAGCAGAATTACTTTTGGATATTGAATCTGTAATTACCTTTTATTGT AAATCACGTAACATTAAATATAGCACATCCCTTAGCTGGATACATCTACTGAAACCATTGGTGCATCTT CAACTGCCACGCAGCGATTTATACAACTGCTTTTATGCCATAATGAATAAGTACATTCCCAGGGATTGT TCCCAGAAAGGGAGACCATTTCATCTCTTCAGGTTGCTCATCCAATACCATGAGCCTGAGCTTTGTTCT TATCTTGATACAAAGAAAATTACTCCAGACTCCTATGCACTCAACTGGCTTGGAAGTCTTTTTGCATGT TACTGTTCCACTGAAGTCACTCAGGCAATATGGGATGGATATCTACAACAAGCAGATCCATTTTTTATT TATTTCTTAATGTTAATTATCCTTGTTAATGCAAAAGAAGTTATTTTAACACAAGAGTCAGACAGCAAA GAAGAAGTTATCAAGTTCTTGGAAAATACTCCATCCAGTCTGAATATAGAAGATATAGAAGACCTTTTC TCTCTGGCTCAGTATTATTGCAGCAAAACACCGGCTTCTTTTAGGAAGGATAATCACCATCTCTTTGGT AGTACTTTGTTGGGAATTAAGGATGATGATGCAGATCTGAGTCAGGCTCTTTGTCTGGCCATCTCCGTG TCAGAGATCCTTCAAGCGAATCAGCTACAAGGGGAAGGAGTCCGGTTCTTTGTGGTGGATTGCCGTCCT GCAGAACAATATAATGCTGGGCATTTATCAACTGCTTTCCACTTAGATTCAGACCTGATGCTTCAGAAT CCATCTGAGTTTGCACAGTCAGTAAAATCCTTGCTGGAAGCACAGAAGCAGTCCATTGAGTCTGGCTCC ATAGCTGGTGGGGAGCACCTCTGTTTTATGGGCAGTGGCAGGGAGGAAGAAGACATGTATATGAACATG GTCCTGGCACACTTTTTACAGAAAAACAAAGAATATGTGAGTATTGCCAGTGGAGGATTTATGGCACTG CAGCAGCACCTGGCAGACATTAATGTGGATGGACCAGAAAATGGATATGGCCATTGGATTGCTAGTACC TCAGGCTCAAGGAGCAGTATCAATTCTGTTGATGGTGAATCTCCTAATGGCTCAAGTGATAGAGGAATG AAATCACTAGTAAATAAAATGACTGTGGCTTTGAAGACAAAATCCGTTAATGTCAGGGAAAAAGTTATC AGTTTTATAGAGAATACATCAACTCCTGTGGATCGAATGTCTTTCAATCTTCCTTGGCCAGACAGATCA TGTACAGAGCGGCATGTGAGCAGCAGTGACAGAGTGGGCAAGCCTTACCGTGGCGTAAAGCCTGTTTTC AGCATTGGGGATGAAGAAGAATACGACACAGATGAAATTGACAGTTCTTCAATGTCAGATGATGATAGA AAAGAGGTTGTAAACATTCAGACTTGGATAAACAAACCAGATGTCAAACATCATTTTCCTTGTAAAGAA GTAAAAGAAAGTGGACACATGTTTCCCAGTCATCTGTTGGTTACTGCAACACATATGTACTGTTTAAGG GAGATTGTTTCACGGAAAGGATTGGCTTATATACAGTCTCGACAAGCGCTGAATTCTGTAGTTAAAATT ACATCCAAAAAAAAACATCCTGAACTCATTACCTTCAAGTATGGAAATAGCAGTGCTTCAGGAATAGAA ATCTTGGCAATCGAAAGGTATTTGATTCCAAATGCAGGGGATGCAACTAAAGCCATAAAACAGCAGATC ATGAAAGTTTTGGATGCTTTGGAAAGTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001199198
Insert Size	2100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001199198.2
RefSeq Size	3886 bp
RefSeq ORF	2100 bp
Locus ID	55773
UniProt ID	Q9NUY8
Protein Families	Druggable Genome
MW	78.3 kDa
Gene Summary	Putative Rab GTPase-activating protein which plays a role in vesicular trafficking (PubMed:28823707). Involved in endosome-to-Golgi trafficking. Acts as a bridging protein by binding simultaneously to golgins, including GOLGA1 and GOLGA4, located at the trans-Golgi, and to the WASH complex, located on endosome-derived vesicles (PubMed:29084197, PubMed:29426865). Together with WDR11 complex facilitates the golgin-mediated capture of vesicles generated using AP-1 (PubMed:29426865). Plays a role in brain development, including in cortical neuron positioning (By similarity). May also be important for neurite outgrowth, possibly through its involvement in membrane trafficking and cargo delivery, 2 processes that are essential for axonal and dendritic growth (By similarity). May act as a general inhibitor of innate immunity signaling, strongly inhibiting multiple TLR and dectin/CLEC7A-signaling pathways. Does not alter initial activation events, but instead affects maintenance of inflammatory gene expression several hours after bacterial lipopolysaccharide (LPS) challenge (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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