KIFAP3 (NM_001204517) Human Tagged ORF Clone
CAT#: RC234831
- TrueORF®
KIFAP3 (Myc-DDK tagged) - Homo sapiens kinesin-associated protein 3 (KIFAP3), transcript variant 4
ORF Plasmid: tGFP
"NM_001204517" in other vectors (2)
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CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | dJ190I16.1; FLA3; KAP-1; KAP-3; KAP3; SMAP; Smg-GDS |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234831 representing NM_001204517
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACCCCATGTTGGGAGAACGAAAAGAATGTCAAAAAATCATTCGACTTAAGAGTCTCAATGCCA ACACAGATATAACTTCCCTGGCAAGGAAGGTGGTTGAAGAATGTAAACTCATTCATCCTTCAAAACTAAA TGAGGTAGAACAGCTGTTGTACTATCTACAGAACCGCCGTGATTCATTGTCAGGAAAAGAGAAAAAAGAA AAATCAAGCAAGCCTAAAGATCCACCTCCTTTTGAAGGAATGGAGATTGATGAAGTTGCTAACATTAATG ACATGGATGAATATATTGAGTTATTATATGAAGATATTCCTGACAAAGTTCGGGGTTCTGCTTTGATCCT GCAGCTTGCTCGAAATCCTGATAACTTGGAAGAACTACTATTGAATGAAACTGCCCTTGGTGCATTAGCA AGGGTCCTGAGAGAAGACTGGAAGCAAAGTGTCGAGTTAGCTACAAACATAATTTACATCTTTTTTTGTT TCTCCAGCTTTTCTCAATTTCATGGACTTATTACTCACTATAAAATTGGAGCTCTGTGTATGAATATTAT TGATCATGAGTTAAAAAGACATGAGCTTTGGCAAGAAGAACTCTCAAAGAAGAAGAAAGCTGTTGATGAA GACCCTGAAAACCAAACCTTGAGAAAGGATTATGAAAAAACCTTTAAAAAGTACCAGGGGCTTGTGGTAA AACAGGAACAGCTATTACGAGTTGCTCTTTATTTGCTTCTGAATCTTGCTGAGGATACTCGTACCGAACT GAAAATGAGGAACAAGAACATAGTTCACATGTTGGTGAAAGCCCTTGATCGGGACAATTTTGAGCTGCTA ATTTTAGTTGTGTCATTCTTGAAGAAACTCAGCATTTTTATGGAGAATAAAAATGATATGGTGGAAATGG ATATTGTTGAAAAACTGGTGAAAATGATACCTTGTGAGCATGAAGACCTGCTGAATATCACCCTCCGACT TTTACTAAACCTATCCTTTGACACAGGACTGAGGAATAAGATGGTACAAGTTGGACTGCTTCCCAAGCTC ACTGCACTCCTAGGCAATGACAACTACAAACAAATAGCAATGTGTGTTCTTTACCACATAAGCATGGATG ACCGCTTTAAATCAATGTTTGCATACACTGACTGTATACCACAGTTAATGAAGATGCTGTTTGAATGTTC AGATGAACGAATTGACTTGGAACTCATTTCTTTCTGCATTAATCTTGCTGCTAACAAAAGAAATGTACAG CTTATCTGTGAAGGAAATGGGCTGAAGATGCTCATGAAGAGGGCTCTGAAGTTTAAGGATCCATTGCTGA TGAAAATGATTAGAAACATTTCTCAGCATGATGGACCAACTAAAAATCTGTTTATTGATTATGTTGGGGA CCTTGCAGCCCAGATCTCTAATGATGAAGAAGAGGAGTTTGTGATTGAATGTTTGGGAACTCTTGCAAAC TTGACCATTCCAGACTTAGACTGGGAATTGGTTCTTAAAGAATATAAGTTGGTTCCATACCTCAAGGATA AACTAAAACCAGGTGCTGCAGAAGATGATCTTGTTTTAGAAGTGGTTATAATGATTGGAACTGTATCCAT GGATGACTCTTGTGCTGCATTGCTAGCCAAATCTGGCATAATCCCTGCACTCATTGAATTGCTAAATGCT CAACAAGAAGATGATGAATTTGTGTGTCAGATAATTTATGTCTTCTACCAGATGGTTTTCCACCAAGCCA CAAGAGACGTCATAATCAAGGAAACACAGGCTCCAGCATATCTCATAGACCTAATGCATGATAAGAATAA TGAAATCCGAAAGGTCTGTGATAATACATTAGATATTATAGCGGAATATGATGAAGAATGGGCTAAGAAA ATTCAGAGTGAAAAGTTTCGCTGGCATAACTCTCAGTGGCTGGAGATGGTAGAGAGTCGTCAGATGGATG AGAGTGAGCAGTACTTGTATGGTGATGATCGAATTGAGCCATACATTCATGAAGGAGATATTCTCGAAAG ACCTGACCTTTTCTACAACTCAGATGGATTAATTGCCTCTGAAGGAGCCATAAGTCCCGATTTCTTCAAT GATTACCACCTTCAAAATGGAGATGTTGTTGGGCAGCATTCATTTCCTGGCAGCCTTGGAATGGATGGCT TTGGCCAACCAGTTGGCATTCTTGGACGCCCTGCCACAGCATATGGATTCCGCCCTGATGAACCTTACTA CTATGGCTATGGATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234831 representing NM_001204517
Red=Cloning site Green=Tags(s) MGDPMLGERKECQKIIRLKSLNANTDITSLARKVVEECKLIHPSKLNEVEQLLYYLQNRRDSLSGKEKKE KSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALA RVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDE DPENQTLRKDYEKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELL ILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKL TALLGNDNYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQ LICEGNGLKMLMKRALKFKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISNDEEEEFVIECLGTLAN LTIPDLDWELVLKEYKLVPYLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNA QQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKK IQSEKFRWHNSQWLEMVESRQMDESEQYLYGDDRIEPYIHEGDILERPDLFYNSDGLIASEGAISPDFFN DYHLQNGDVVGQHSFPGSLGMDGFGQPVGILGRPATAYGFRPDEPYYYGYGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001204517 |
ORF Size | 2256 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001204517.1, NP_001191446.1 |
RefSeq Size | 4120 bp |
RefSeq ORF | 2259 bp |
Locus ID | 22920 |
UniProt ID | Q92845 |
Protein Families | Druggable Genome |
MW | 87.2 kDa |
Gene Summary | The small G protein GDP dissociation stimulator (smg GDS) is a regulator protein having two activities on a group of small G proteins including the Rho and Rap1 family members and Ki-Ras; one is to stimulate their GDP/GTP exchange reactions, and the other is to inhibit their interactions with membranes. The protein encoded by this gene contains 9 'Armadillo' repeats and interacts with the smg GDS protein through these repeats. This protein, which is highly concentrated around the endoplasmic reticulum, is phosphorylated by v-src, and this phosphorylation reduces the affinity of the protein for smg GDS. It is thought that this protein serves as a linker between human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and that it plays a role in the interaction of chromosomes with an ATPase motor protein. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
Documents
Product Manuals |
FAQs |
SDS |