AP180 (SNAP91) (NM_001256717) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AP180; CALM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234925 representing NM_001256717 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGCGTTCAAGAATAATAACAATTTGATCCAGGCTACCAACGAGACCAATGTTAATATTCCTCAGA TGGCCGACACTCTCTTTGAGCGGGCAACAAACAGTAGCTGGGTGGTTGTGTTTAAGGCTTTAGTGACAAC ACATCATCTCATGGTGCATGGAAATGAGAGATTTATTCAATATTTGGCTTCTAGAAATACACTATTCAAT CTCAGCAATTTTTTGGACAAAAGTGGATCCCATGGTTATGATATGTCTACCTTCATAAGGCGCTATAGTA GATATTTGAATGAAAAGGCTTTTTCTTACAGACAGATGGCCTTTGATTTTGCCAGGGTGAAGAAAGGGGC CGATGGTGTAATGAGGACAATGGCTCCCGAAAAGCTGCTAAAGAGTATGCCAATACTACAGGGACAAATT GATGCACTGCTTGAATTTGATGTGCATCCAAATGAACTAACAAATGGTGTCATAAATGCAGCATTTATGC TTCTTTTCAAAGATCTTATCAAACTTTTTGCTTGCTACAATGATGGTGTTATTAACTTACTCGAAAAGTT TTTTGAAATGAAGAAAGGACAATGTAAAGATGCTCTAGAAATTTACAAACGATTTCTAACTAGAATGACA CGAGTGTCTGAATTTCTCAAGGTTGCAGAGCAAGTTGGTATTGATAAAGGTGACATTCCTGACCTCACAC AGGCTCCCAGCAGTCTTATGGAGACGCTTGAACAGCATCTAAATACATTAGAAGGAAAGAAACCTGGAAA CAAATCTGGTGCTCCCTCTCCATTAAGTAAGTCTTCTCCAGCCACAACTGTTACGTCTCCTAATTCTACA CCAGCTAAAACTATTGACACATCCCCACCGGTTGATTTATTTGCAACTGCATCTGCGGCTGTCCCAGTCA GCACTTCTAAACCATCTAGTGATCTCCTGGACCTCCAGCCAGACTTTTCCTCTGGAGGGGCAGCAGCAGC CGCAGCACCAGCACCACCACCACCTGCTGGAGGAGCCACTGCATGGGGAGACCTTTTGGGAGAGGATTCT TTGGCTGCACTTTCCTCTGTTCCCTCTGAAGCACAGATTTCAGATCCATTTGCACCAGAACCTACCCCTC CTACTACAACTGCTGAAATTGCAACTGCCTCAGCTTCTGCCTCCACTACTACAACTGTTACTGCTGTCAC TGCTGAAGTGGATCTCTTTGGAGATGCCTTTGCAGCTTCTCCTGGGGAGGCCCCTGCAGCATCCGAAGGG GCCGCCGCACCAGCTACCCCAACCCCTGTAGCAGCAGCACTTGATGCATGTTCAGGAAATGACCCCTTTG CCCCGTCTGAAGGTAGTGCAGAGGCTGCACCTGAGCTGGACCTCTTTGCAATGAAGCCACCTGAGACCAG TGTTCCTGTAGTTACCCCTACAGCTAGCACAGCCCCTCCAGTTCCCGCAACTGCTCCTTCTCCTGCTCCT GCCGTTGCAGCTGCTGCTGCTGCCACTACTGCTGCCACCGCCGCTGCCACCACCACTACCACCACCTCCG CTGCCACCGCCACCACTGCTCCTCCTGCTCTAGATATCTTTGGTGATGCTTTCTCCTCTCCACCACAAGG GGCCTCTCCTGTGCCTGAGAGTTCTCTCACTGCTGACCTCTTATCTGATGCATTTGGAAGTAGTGCTTCT GAACCCCAACCTGCATCTCAGGCTGCTTCTAGTTCATCAGCATCGGCAGACCTACTAGCTGGATTTGGGG GTTCTTTCATGGCGCCTTCCCCATCTCCAGTGACTCCAGCTCAGAATAACCTGCTACAGCCCAATTTTGA GGCAGCTTTTGGGACAACGCCTTCAACTTCCAGCAGCAGCTCCTTTGATCCATCAGTGTTTGATGGTCTA GGTGATCTTTTGATGCCAACCATGGCACCAGCTGGGCAGCCTGCACCTGTCTCAATGGTACCACCCAGTC CTGCAATGGCAGCCAGCAAAGCCCTTGGAAGTGATCTTGATTCATCTCTTGCCAGCTTAGTAGGCAATCT TGGAATTTCTGGTACCACAACAAAAAAGGGAGATCTTCAGTGGAATGCTGGAGAGAAAAAGTTGACTGGT GGAGCCAACTGGCAGCCTAAAGTAGCTCCAGCAACCTGGTCAGCAGGCGTTCCACCAAGTGCACCTTTGC AAGGAGCTGTACCTCCAACCAGTTCAGTTCCTCCTGTTGCCGGGGCCCCATCGGTTGGACAACCTGGAGC AGGATTTGGAATGCCTCCTGCTGGGACAGGCATGCCCATGATGCCTCAGCAGCCGGTCATGTTTGCACAG CCCATGATGAGGCCCCCCTTTGGAGCTGCCGCTGTACCTGGCACGCAGCTTTCTCCAAGCCCTACACCTG CCAGTCAGAGTCCCAAGAAACCTCCAGCAAAGGACCCATTAGCGGATCTTAACATCAAGGATTTCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234925 representing NM_001256717 Red=Cloning site Green=Tags(s) MRAFKNNNNLIQATNETNVNIPQMADTLFERATNSSWVVVFKALVTTHHLMVHGNERFIQYLASRNTLFN LSNFLDKSGSHGYDMSTFIRRYSRYLNEKAFSYRQMAFDFARVKKGADGVMRTMAPEKLLKSMPILQGQI DALLEFDVHPNELTNGVINAAFMLLFKDLIKLFACYNDGVINLLEKFFEMKKGQCKDALEIYKRFLTRMT RVSEFLKVAEQVGIDKGDIPDLTQAPSSLMETLEQHLNTLEGKKPGNKSGAPSPLSKSSPATTVTSPNST PAKTIDTSPPVDLFATASAAVPVSTSKPSSDLLDLQPDFSSGGAAAAAAPAPPPPAGGATAWGDLLGEDS LAALSSVPSEAQISDPFAPEPTPPTTTAEIATASASASTTTTVTAVTAEVDLFGDAFAASPGEAPAASEG AAAPATPTPVAAALDACSGNDPFAPSEGSAEAAPELDLFAMKPPETSVPVVTPTASTAPPVPATAPSPAP AVAAAAAATTAATAAATTTTTTSAATATTAPPALDIFGDAFSSPPQGASPVPESSLTADLLSDAFGSSAS EPQPASQAASSSSASADLLAGFGGSFMAPSPSPVTPAQNNLLQPNFEAAFGTTPSTSSSSSFDPSVFDGL GDLLMPTMAPAGQPAPVSMVPPSPAMAASKALGSDLDSSLASLVGNLGISGTTTKKGDLQWNAGEKKLTG GANWQPKVAPATWSAGVPPSAPLQGAVPPTSSVPPVAGAPSVGQPGAGFGMPPAGTGMPMMPQQPVMFAQ PMMRPPFGAAAVPGTQLSPSPTPASQSPKKPPAKDPLADLNIKDFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256717
ORF Size	2448 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256717.1, NP_001243646.1
RefSeq Size	4529 bp
RefSeq ORF	2451 bp
Locus ID	9892
UniProt ID	O60641
MW	83.8 kDa
Gene Summary	Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats (By similarity).[UniProtKB/Swiss-Prot Function]

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