AP180 (SNAP91) (NM_001256717) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AP180; CALM
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332449 representing NM_001256717. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGGGCGTTCAAGAATAATAACAATTTGATCCAGGCTACCAACGAGACCAATGTTAATATTCCTCAG ATGGCCGACACTCTCTTTGAGCGGGCAACAAACAGTAGCTGGGTGGTTGTGTTTAAGGCTTTAGTGACA ACACATCATCTCATGGTGCATGGAAATGAGAGATTTATTCAATATTTGGCTTCTAGAAATACACTATTC AATCTCAGCAATTTTTTGGACAAAAGTGGATCCCATGGTTATGATATGTCTACCTTCATAAGGCGCTAT AGTAGATATTTGAATGAAAAGGCTTTTTCTTACAGACAGATGGCCTTTGATTTTGCCAGGGTGAAGAAA GGGGCCGATGGTGTAATGAGGACAATGGCTCCCGAAAAGCTGCTAAAGAGTATGCCAATACTACAGGGA CAAATTGATGCACTGCTTGAATTTGATGTGCATCCAAATGAACTAACAAATGGTGTCATAAATGCAGCA TTTATGCTTCTTTTCAAAGATCTTATCAAACTTTTTGCTTGCTACAATGATGGTGTTATTAACTTACTC GAAAAGTTTTTTGAAATGAAGAAAGGACAATGTAAAGATGCTCTAGAAATTTACAAACGATTTCTAACT AGAATGACACGAGTGTCTGAATTTCTCAAGGTTGCAGAGCAAGTTGGTATTGATAAAGGTGACATTCCT GACCTCACACAGGCTCCCAGCAGTCTTATGGAGACGCTTGAACAGCATCTAAATACATTAGAAGGAAAG AAACCTGGAAACAAATCTGGTGCTCCCTCTCCATTAAGTAAGTCTTCTCCAGCCACAACTGTTACGTCT CCTAATTCTACACCAGCTAAAACTATTGACACATCCCCACCGGTTGATTTATTTGCAACTGCATCTGCG GCTGTCCCAGTCAGCACTTCTAAACCATCTAGTGATCTCCTGGACCTCCAGCCAGACTTTTCCTCTGGA GGGGCAGCAGCAGCCGCAGCACCAGCACCACCACCACCTGCTGGAGGAGCCACTGCATGGGGAGACCTT TTGGGAGAGGATTCTTTGGCTGCACTTTCCTCTGTTCCCTCTGAAGCACAGATTTCAGATCCATTTGCA CCAGAACCTACCCCTCCTACTACAACTGCTGAAATTGCAACTGCCTCAGCTTCTGCCTCCACTACTACA ACTGTTACTGCTGTCACTGCTGAAGTGGATCTCTTTGGAGATGCCTTTGCAGCTTCTCCTGGGGAGGCC CCTGCAGCATCCGAAGGGGCCGCCGCACCAGCTACCCCAACCCCTGTAGCAGCAGCACTTGATGCATGT TCAGGAAATGACCCCTTTGCCCCGTCTGAAGGTAGTGCAGAGGCTGCACCTGAGCTGGACCTCTTTGCA ATGAAGCCACCTGAGACCAGTGTTCCTGTAGTTACCCCTACAGCTAGCACAGCCCCTCCAGTTCCCGCA ACTGCTCCTTCTCCTGCTCCTGCCGTTGCAGCTGCTGCTGCTGCCACTACTGCTGCCACCGCCGCTGCC ACCACCACTACCACCACCTCCGCTGCCACCGCCACCACTGCTCCTCCTGCTCTAGATATCTTTGGTGAT GCTTTCTCCTCTCCACCACAAGGGGCCTCTCCTGTGCCTGAGAGTTCTCTCACTGCTGACCTCTTATCT GATGCATTTGGAAGTAGTGCTTCTGAACCCCAACCTGCATCTCAGGCTGCTTCTAGTTCATCAGCATCG GCAGACCTACTAGCTGGATTTGGGGGTTCTTTCATGGCGCCTTCCCCATCTCCAGTGACTCCAGCTCAG AATAACCTGCTACAGCCCAATTTTGAGGCAGCTTTTGGGACAACGCCTTCAACTTCCAGCAGCAGCTCC TTTGATCCATCAGTGTTTGATGGTCTAGGTGATCTTTTGATGCCAACCATGGCACCAGCTGGGCAGCCT GCACCTGTCTCAATGGTACCACCCAGTCCTGCAATGGCAGCCAGCAAAGCCCTTGGAAGTGATCTTGAT TCATCTCTTGCCAGCTTAGTAGGCAATCTTGGAATTTCTGGTACCACAACAAAAAAGGGAGATCTTCAG TGGAATGCTGGAGAGAAAAAGTTGACTGGTGGAGCCAACTGGCAGCCTAAAGTAGCTCCAGCAACCTGG TCAGCAGGCGTTCCACCAAGTGCACCTTTGCAAGGAGCTGTACCTCCAACCAGTTCAGTTCCTCCTGTT GCCGGGGCCCCATCGGTTGGACAACCTGGAGCAGGATTTGGAATGCCTCCTGCTGGGACAGGCATGCCC ATGATGCCTCAGCAGCCGGTCATGTTTGCACAGCCCATGATGAGGCCCCCCTTTGGAGCTGCCGCTGTA CCTGGCACGCAGCTTTCTCCAAGCCCTACACCTGCCAGTCAGAGTCCCAAGAAACCTCCAGCAAAGGAC CCATTAGCGGATCTTAACATCAAGGATTTCTTGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256717
Insert Size	2451 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256717.1
RefSeq Size	4529 bp
RefSeq ORF	2451 bp
Locus ID	9892
UniProt ID	O60641
MW	83.4 kDa
Gene Summary	Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a.

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