GRIA1 (NM_001258019) Human Tagged ORF Clone
CAT#: RC234938
- TrueORF®
GRIA1 (Myc-DDK tagged) - Homo sapiens glutamate receptor, ionotropic, AMPA 1 (GRIA1), transcript variant 3
ORF Plasmid: tGFP
"NM_001258019" in other vectors (2)
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CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | GluA1; GLUH1; GLUR1; GLURA; HBGR1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234938 representing NM_001258019
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCACATTTTTGCCTTCTTCTGCACCGGTTTCCTAGGCGCGGTAGTAGGTGCCAATTTCCCCAACA ATATCCAGATCGGGGGATTATTTCCAAACCAGCAGTCACAGGAACATGCTGCTTTTAGATTTGCTTTGTC GCAACTCACAGAGCCCCCGAAGCTGCTCCCCCAGATTGATATTGTGAACATCAGCGACAGCTTTGAGATG ACCTATAGATGCTTATCCGTCCTGCAGAAAGTCCTGGATACAGCTGCTGAGAAGAACTGGCAGGTGACAG CAGTCAACATTTTGACAACCACAGAGGAGGGATACCGGATGCTCTTTCAGGACCTGGAGAAGAAAAAGGA GCGGCTGGTGGTGGTGGACTGTGAATCAGAACGCCTCAATGCTATCTTGGGCCAGATTATAAAGCTAGAG AAGAATGGCATCGGCTACCACTACATTCTTGCAAATCTGGGCTTCATGGACATTGACTTAAACAAATTCA AGGAGAGTGGCGCCAATGTGACAGGTTTCCAGCTGGTGAACTACACAGACACTATTCCGGCCAAGATCAT GCAGCAGTGGAAGAATAGTGATGCTCGAGACCACACACGGGTGGACTGGAAGAGACCCAAGTACACCTCT GCGCTCACCTACGATGGGGTGAAGGTGATGGCTGAGGCTTTCCAGAGCCTGCGGAGGCAGAGAATTGATA TATCTCGCCGGGGGAATGCTGGGGATTGTCTGGCTAACCCAGCTGTTCCCTGGGGCCAAGGGATCGACAT CCAGAGAGCTCTGCAGCAGGTGCGATTTGAAGGTTTAACAGGAAACGTGCAGTTTAATGAGAAAGGACGC CGGACCAACTACACGCTCCACGTGATTGAAATGAAACATGACGGCATCCGAAAGATTGGTTACTGGAATG AAGATGATAAGTTTGTCCCTGCAGCCACCGATGCCCAAGCTGGGGGCGATAATTCAAGTGTTCAGAACAG AACATACATCGTCACAACAATCCTAGAAGATCCTTATGTGATGCTCAAGAAGAACGCCAATCAGTTTGAG GGCAATGACCGTTACGAGGGCTACTGTGTAGAGCTGGCGGCAGAGATTGCCAAGCACGTGGGCTACTCCT ACCGTCTGGAGATTGTCAGTGATGGAAAATACGGAGCCCGAGACCCTGACACGAAGGCCTGGAATGGCAT GGTGGGAGAGCTGGTCTATGGAAGAGCAGATGTGGCTGTGGCTCCCTTAACTATCACTTTGGTCCGGGAA GAAGTTATAGATTTCTCCAAACCATTTATGAGTTTGGGGATCTCCATCATGATTAAAAAACCACAGAAAT CCAAGCCGGGTGTCTTCTCCTTCCTTGATCCTTTGGCTTATGAGATTTGGATGTGCATTGTTTTTGCCTA CATTGGAGTGAGTGTTGTCCTCTTCCTGGTCAGCCGCTTCAGTCCCTATGAATGGCACAGTGAAGAGTTT GAGGAAGGACGGGACCAGACAACCAGTGACCAGTCCAATGAGTTTGGGATATTCAACAGTTTGTGGTTCT CCCTGGGAGCCTTCATGCAGCAAGGATGTGACATTTCTCCCAGGTCCCTGTCTGGTCGCATCGTTGGTGG CGTCTGGTGGTTCTTCACCTTAATCATCATCTCCTCATATACAGCCAATCTGGCCGCCTTCCTGACCGTG GAGAGGATGGTGTCTCCCATTGAGAGTGCAGAGGACCTAGCGAAGCAGACAGAAATTGCCTACGGGACGC TGGAAGCAGGATCTACTAAGGAGTTCTTCAGGAGGTCTAAAATTGCTGTGTTTGAGAAGATGTGGACATA CATGAAGTCAGCAGAGCCATCAGTTTTTGTGCGGACCACAGAGGAGGGGATGATTCGAGTGAGGAAATCC AAAGGCAAATATGCCTACCTCCTGGAGTCCACCATGAATGAGTACATTGAGCAGCGGAAACCCTGTGACA CCATGAAGGTGGGAGGTAACTTGGATTCCAAAGGCTATGGCATTGCAACACCCAAGGGGTCTGCCCTGAG AAATCCAGTAAACCTGGCAGTGTTAAAACTGAACGAGCAGGGGCTTTTGGACAAATTGAAAAACAAATGG TGGTACGACAAGGGCGAGTGCGGCAGCGGGGGAGGTGATTCCAAGGACAAGACAAGCGCTCTGAGCCTCA GCAATGTGGCAGGCGTGTTCTACATCCTGATCGGAGGACTTGGACTAGCCATGCTGGTTGCCTTAATCGA GTTCTGCTACAAATCCCGTAGTGAATCCAAGCGGATGAAGGGTTTTTGTTTGATCCCACAGCAATCCATC AACGAAGCCATACGGACATCGACCCTCCCCCGCAACAGCGGGGCAGGAGCCAGCAGCGGCGGCAGTGGAG AGAATGGTCGGGTGGTCAGCCATGACTTCCCCAAGTCCATGCAATCGATTCCTTGCATGAGCCACAGTTC AGGGATGCCCTTGGGAGCCACGGGATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234938 representing NM_001258019
Red=Cloning site Green=Tags(s) MQHIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEM TYRCLSVLQKVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIIKLE KNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPAKIMQQWKNSDARDHTRVDWKRPKYTS ALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGR RTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFE GNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVRE EVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEF EEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTV ERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKS KGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKW WYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSI NEAIRTSTLPRNSGAGASSGGSGENGRVVSHDFPKSMQSIPCMSHSSGMPLGATGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001258019 |
ORF Size | 2478 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001258019.1, NP_001244948.1 |
RefSeq Size | 5507 bp |
RefSeq ORF | 2481 bp |
Locus ID | 2890 |
UniProt ID | P42261 |
Protein Families | Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane |
Protein Pathways | Amyotrophic lateral sclerosis (ALS), Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction |
MW | 92.6 kDa |
Gene Summary | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |