KIF18B (NM_001264573) Human Tagged ORF Clone
CAT#: RC234940
- TrueORF®
KIF18B (Myc-DDK tagged) - Homo sapiens kinesin family member 18B (KIF18B), transcript variant 2
ORF Plasmid: tGFP
"NM_001264573" in other vectors (2)
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CNY 7,130.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC234940 representing NM_001264573
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGGAGGACAGCACGCTGCAAGTAGTGGTACGGGTGCGGCCCCCCACCCCTCGGGAGCTGGACA GTCAGCGGCGGCCAGTGGTTCAGGTGGTGGACGAGCGGGTGCTGGTGTTTAACCCTGAGGAGCCCGATGG AGGGTTCCCTGGCCTGAAATGGGGTGGCACCCATGATGGCCCCAAGAAGAAGGGCAAAGACCTGACGTTT GTCTTTGACCGGGTCTTTGGCGAGGCGGCCACCCAACAGGACGTGTTCCAGCACACCACGCACAGCGTCC TGGACAGCTTCCTCCAGGGCTACAACTGCTCAGTGTTTGCCTACGGGGCCACCGGGGCTGGGAAGACACA CACCATGCTGGGAAGGGAGGGGGACCCCGGCATCATGTACCTGACCACCGTGGAACTGTACAGGCGCCTG GAGGCCCGCCAGCAGGAGAAGCACTTCGAGGTGCTCATCAGCTACCAGGAGGTGTATAATGAACAGATCC ATGACCTCCTGGAGCCCAAGGGGCCCCTTGCCATCCGCGAGGACCCCGACAAGGGGGTGGTGGTGCAAGG ACTTTCTTTCCACCAGCCAGCCTCAGCCGAGCAGCTGCTGGAGATACTGACCAGGGGGAACCGTAACCGC ACGCAGCACCCCACTGATGCCAACGCGACTTCCTCCCGCTCCCATGCCATCTTCCAGATCTTTGTGAAGC AGCAGGACCGGGTTCCAGGACTGACCCAGGCTGTCCAGGTGGCCAAGATGAGCCTGATTGACCTGGCTGG CTCAGAGCGGGCATCCAGCACCCATGCGAAGGGGGAGCGGCTGCGGGAGGGGGCCAACATCAACCGCTCT CTGCTGGCGCTCATCAACGTCCTCAATGCCTTGGCCGATGCAAAGGGCCGCAAGACCCATGTGCCCTACC GGGACAGCAAACTGACCCGCCTGCTCAAAGACTCCCTCGGGGGCAACTGCCGCACAGTGATGATCGCTGC CATCAGCCCCTCCAGCCTGACCTACGAGGACACGTACAACACCCTCAAATATGCCGACCGGGCCAAGGAG ATCAGGCTCTCGCTGAAGAGCAATGTGACCAGCCTGGACTGTCACATCAGCCAGTATGCTACCATCTGCC AACAGCTCCAGGCTGAGGTAGCCGCTCTGAGGAAGAAGCTCCAAGTGTATGAGGGGGGAGGCCAGCCCCC ACCACAGGACCTCCCAGGATCTCCCAAGTCGGGACCACCACCAGAACACCTTCCCAGCTCCCCCTTGCCA CCCCACCCTCCCAGCCAGCCCTGCACCCCAGAGCTCCCTGCAGGGCCTAGAGCCCTTCAAGAGGAGAGTC TGGGGATGGAGGCCCAGGTGGAGAGGGCCATGGAAGGGAACTCTTCAGACCAGGAGCAGTCCCCAGAGGA TGAGGATGAAGGCCCAGCTGAGGAGGTTCCAACCCAGATGCCAGAGCAGAACCCCACACATGCACTGCCA GAGTCCCCTCGCCTGACCCTGCAGCCCAAGCCAGTCGTGGGCCACTTCTCAGCACGGGAACTGGATGGGG ACCGTTCTAAGCAGTTGGCCCTAAAGGTGCTGTGCGTTGCCCAGCGGCAGTACTCCCTGCTCCAAGCAGC CAACCTCCTGACGCCCGACATGATCACAGAGTTTGAGACCCTACAGCAGCTGGTGCAAGAGGAAAAAATT GAGCCTGGGGCAGAGGCCTTGAGGACTTCAGGCCTGGCCAGGGGGGCACCTCTGGCTCAGGAGCTGTGTT CAGAGTCAATCCCTGTGCCGTCTCCTCTCTGCCCAGAGCCTCCAGGATACACTGGCCCTGTGACCCGGAC TATGGCGAGGCGACTGAGTGGCCCCCTGCACACCCTGGGAATCCCGCCTGGACCCAACTGCACCCCAGCC CAGGGGTCCCGATGGCCCATGGAGAAGAAGAGGAGGAGACCAAGCGCCTTGGAGGCAGACAGTCCCATGG CCCCAAAGCGGGGCACCAAGCGCCAGCGCCAGTCCTTCCTGCCCTGCCTAAGGAGAGGGTCTCTGCCTGA CACCCAACCTTCACAGGGGCCCAGCACCCCCAAAGGAGAAAGGGCCTCCTCCCCCTGCCATTCCCCTCGC GTTTGCCCAGCCACAGTCATCAAAAGCCGGGTGCCCCTGGGCCCTTCCGCCATGCAGAACTGCTCCACCC CGCTGGCTCTGCCCACTCGAGACCTCAATGCCACCTTTGATCTCTCTGAGGAGCCTCCCTCAAAGCCCAG TTTCCATGAATGCATTGGCTGGGACAAAATACCCCAGGAGCTGAGCAGGCTGGACCAGCCCTTCATCCCC AGGGCACCTGTGCCCCTGTTCACCATGAAGGGCCCCAAGCCAACATCTTCCCTCCCTGGGACCTCTGCCT GCAAGAAGAAGCGCGTTGCGAGTTCCTCAGTCTCCCATGGCCGCAGCCGCATCGCCCGCCTCCCCAGCAG CACTTTGAAGAGGCCAGCTGGGCCCCTTGTACTCCCAGGTGACTGGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC234940 representing NM_001264573
Red=Cloning site Green=Tags(s) MAVEDSTLQVVVRVRPPTPRELDSQRRPVVQVVDERVLVFNPEEPDGGFPGLKWGGTHDGPKKKGKDLTF VFDRVFGEAATQQDVFQHTTHSVLDSFLQGYNCSVFAYGATGAGKTHTMLGREGDPGIMYLTTVELYRRL EARQQEKHFEVLISYQEVYNEQIHDLLEPKGPLAIREDPDKGVVVQGLSFHQPASAEQLLEILTRGNRNR TQHPTDANATSSRSHAIFQIFVKQQDRVPGLTQAVQVAKMSLIDLAGSERASSTHAKGERLREGANINRS LLALINVLNALADAKGRKTHVPYRDSKLTRLLKDSLGGNCRTVMIAAISPSSLTYEDTYNTLKYADRAKE IRLSLKSNVTSLDCHISQYATICQQLQAEVAALRKKLQVYEGGGQPPPQDLPGSPKSGPPPEHLPSSPLP PHPPSQPCTPELPAGPRALQEESLGMEAQVERAMEGNSSDQEQSPEDEDEGPAEEVPTQMPEQNPTHALP ESPRLTLQPKPVVGHFSARELDGDRSKQLALKVLCVAQRQYSLLQAANLLTPDMITEFETLQQLVQEEKI EPGAEALRTSGLARGAPLAQELCSESIPVPSPLCPEPPGYTGPVTRTMARRLSGPLHTLGIPPGPNCTPA QGSRWPMEKKRRRPSALEADSPMAPKRGTKRQRQSFLPCLRRGSLPDTQPSQGPSTPKGERASSPCHSPR VCPATVIKSRVPLGPSAMQNCSTPLALPTRDLNATFDLSEEPPSKPSFHECIGWDKIPQELSRLDQPFIP RAPVPLFTMKGPKPTSSLPGTSACKKKRVASSSVSHGRSRIARLPSSTLKRPAGPLVLPGDWH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001264573 |
ORF Size | 2499 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001264573.2 |
RefSeq Size | 4308 bp |
RefSeq ORF | 2502 bp |
Locus ID | 146909 |
UniProt ID | Q86Y91 |
Protein Families | Druggable Genome |
MW | 91.5 kDa |
Gene Summary | In complex with KIF2C, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Its major role may be to transport KIF2C and/or MAPRE1 along microtubules.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |