DLGAP1 (NM_001242761) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DAP-1; DAP-1-ALPHA; DAP-1-BETA; DAP1; DLGAP1A; DLGAP1B; GKAP; SAPAP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235046 representing NM_001242761 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGGGCTATCAGGCAGCCGCAGCCATCACCACGGGGTCACCTGCGACTCGGCCTGTGACTCGCTGT CGCACCACTCCGACCGCAAGCCCTACCTGCTGAGCCCAGTGGAGCACCACCCCGCAGACCACCCATACTA CACCCAGCGGAACTCCTTCCAGGCTGAGTGCGTGGGCCCCTTCAGCGACCCGCTGGCCAGCAGCACCTTC CCCCGCAGGCACTACACCTCGCAGCAAGAGCTGAAGGACGAGTGTGCCCTGGTGCCCCGCACCCTGGCCA CCAAGGCGAACCGCATCCCCGCCAACCTGCTGGACCAGTTCGAGCGGCAGCTGCCACTCAGCCGCGATGG CTATCACACCCTGCAGTACAAGCGCACGGCCGTGGAGCACCGCAGCGACAGCCCCGGCCGCATCCGCCAC CTGGTGCACTCGGTCCAGAAGCTCTTCACCAAGTCGCACTCCCTGGAGGGGCCGTCCAAGGGCAGCGTCA ACGGGGGCAAGGCCAGCCCTGACGAGGCGCAGGCGGCGCGCTATGGCAAACGCAGCAAGAGCAAGGAGCG GCGCGCGGAGCCCAAGGCCCGGCCCAGCACCTCCCCGGGCTGGTGGAGCTCGGACGACAACCTGGATGGT GACATGTGCATCTACCACGCCCCCTCGGGCGTGATGACCATGGGCAGGTGCCCCGACCGCTCGGCCTCAC AGTACTTCCTGGAGGCCTACAACACCATCAGCGAGCAGGCGGTGAAGGCCTCCCGGAGCAACAACGACGT CAAGTGCTCCACCTGCGCCAACCTGCCGGTCAGCCTGGACACCCCGCTGCTGAAGAAGAGCGCCTGGTCC TCCACGCTCACCGTGAGCCGGGCCCGGGAGGTTTACCAGAAGGCCTCGGTGAACATGGACCAGGCCATGG TGAAGTCCGAGTCGTGTCAGCAAGAACGCTCCTGCCAGTACCTGCAGGTTCCACAAGATGAATGGACAGG GTACACCCCACGAGGTAAAGATGATGAAATTCCATGCCGAAGAATGCGGAGTGGCAGTTATATCAAGGCC ATGGGGGATGAAGACAGTGGAGACTCAGACACGAGTCCTAAGCCTTCTCCAAAAGTTGCTGCGCGGAGAG AAAGCTATCTCAAGGCTACTCAGCCATCCCTTACAGAACTCACCACACTCAAAATCTCCAATGAACACTC ACCCAAACTCCAGATCCGGAGTCATAGTTACCTGAGGGCAGTGAGTGAAGTCTCCATCAACCGGAGCCTG GACAGCCTGGACCCTGCAGGCTTGCTCACATCACCAAAGTTCCGCTCCAGGAATGAGAGCTACATGCGAG CCATGAGCACCATCAGCCAGGTGAGCGAGATGGAAGTGAACGGGCAGTTCGAGTCCGTGTGCGAGTCCGT GTTCAGCGAGCTGGAGTCGCAGGCCGTGGAAGCGCTGGACCTGCCCATGCCCGGCTGCTTCCGCATGCGG AGCCACAGCTATGTGCGGGCCATTGAGAAAGGCTGCTCCCAGGACGACGAGTGCGTGTCCCTGAGGTCGT CCTCGCCGCCGCGCACCACCACCACCGTTAGGACCATCCAGAGCAGCACGGTGTCATCTTGCATTACAAC ATATAAGAAGACACCACCTCCAGTCCCACCCAGAACTACCACGAAACCTTTCATTTCTATCACAGCCCAG AGTAGCACAGAGTCAGCCCAGGATGCCTACATGGACGGACAGGGCCAGCGAGGAGATATTATCAGCCAGT CTGGACTCAGCAACTCCACCGAGAGCCTGGACAGTATGAAGGCTCTGACAGCCGCCATCGAAGCTGCAAA CGCCCAGATCCATGGCCCTGCCAGTCAACACATGGGCAATAACACTGCCACCGTCACCACCACGACTACC ATAGCCACCGTCACCACGGAGGACAGGAAGAAGGACCACTTTAAGAAAAATCGATGCCTGTCTATCGGGA TACAGGTGGATGATGCTGAAGAACCTGACAAAACAGGGGAGAATAAAGCACCCAGTAAGTTCCAGTCCGT GGGAGTGCAAGTAGAAGAAGAGAAGTGCTTCCGCAGGTTCACTCGATCCAACAGTGTGACGACAGCAGTA CAGGCCGACCTGGACTTCCATGATAATCTGGAAAATTCTCTGGAATCTATAGAGGACAATTCGTGTCCTG GCCCCATGGCCAGACAGTTCTCCCGCGATGCCAGCACCTCCACAGTCAGCATTCAGGGCTCAGGAAACCA TTACCATGCCTGTGCCGCCGATGATGACTTTGACACGGATTTTGACCCCTCTATTCTGCCTCCTCCGGAC CCCTGGATTGACTCTATCACTGAAGACCCTCTGGAGGCCGTGCAAAGGTCAGTGTGCCACCGGGATGGCC ACTGGTTCCTGAAGCTTCTCCAGGCAGAGCGAGACCGCATGGAGGGGTGGTGTCAACAGATGGAGCGGGA AGAACGGGAAAACAACCTGCCCGAAGACATTCTAGGAAAAATCCGAACCGCAGTGGGCAGTGCCCAACTT CTCATGGCCCAGAAATTCTACCAGTTCAGAGAACTGTGTGAAGAAAACCTGAATCCTAATGCTCATCCAA GACCCACCTCCCAGGATTTGGCGGGGTTTTGGGACATGCTGCAGTTGTCCATAGAAAATATTAGTATGAA ATTTGATGAACTTCATCAGTTAAAGGCCAATAATTGGAAACAGATGGATCCTCTTGACAAGAAGGTAGAA CAATGTAGATTTTGTATGGTTCATTTAAAAACCTGCACAAACACTGGACAGTCTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235046 representing NM_001242761 Red=Cloning site Green=Tags(s) MKGLSGSRSHHHGVTCDSACDSLSHHSDRKPYLLSPVEHHPADHPYYTQRNSFQAECVGPFSDPLASSTF PRRHYTSQQELKDECALVPRTLATKANRIPANLLDQFERQLPLSRDGYHTLQYKRTAVEHRSDSPGRIRH LVHSVQKLFTKSHSLEGPSKGSVNGGKASPDEAQAARYGKRSKSKERRAEPKARPSTSPGWWSSDDNLDG DMCIYHAPSGVMTMGRCPDRSASQYFLEAYNTISEQAVKASRSNNDVKCSTCANLPVSLDTPLLKKSAWS STLTVSRAREVYQKASVNMDQAMVKSESCQQERSCQYLQVPQDEWTGYTPRGKDDEIPCRRMRSGSYIKA MGDEDSGDSDTSPKPSPKVAARRESYLKATQPSLTELTTLKISNEHSPKLQIRSHSYLRAVSEVSINRSL DSLDPAGLLTSPKFRSRNESYMRAMSTISQVSEMEVNGQFESVCESVFSELESQAVEALDLPMPGCFRMR SHSYVRAIEKGCSQDDECVSLRSSSPPRTTTTVRTIQSSTVSSCITTYKKTPPPVPPRTTTKPFISITAQ SSTESAQDAYMDGQGQRGDIISQSGLSNSTESLDSMKALTAAIEAANAQIHGPASQHMGNNTATVTTTTT IATVTTEDRKKDHFKKNRCLSIGIQVDDAEEPDKTGENKAPSKFQSVGVQVEEEKCFRRFTRSNSVTTAV QADLDFHDNLENSLESIEDNSCPGPMARQFSRDASTSTVSIQGSGNHYHACAADDDFDTDFDPSILPPPD PWIDSITEDPLEAVQRSVCHRDGHWFLKLLQAERDRMEGWCQQMEREERENNLPEDILGKIRTAVGSAQL LMAQKFYQFRELCEENLNPNAHPRPTSQDLAGFWDMLQLSIENISMKFDELHQLKANNWKQMDPLDKKVE QCRFCMVHLKTCTNTGQSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242761
ORF Size	2787 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242761.1, NP_001229690.1
RefSeq Size	3610 bp
RefSeq ORF	2790 bp
Locus ID	9229
UniProt ID	O14490
MW	104 kDa
Gene Summary	Part of the postsynaptic scaffold in neuronal cells.[UniProtKB/Swiss-Prot Function]

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