TEX14 (NM_001201457) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CT113; SPGF23
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235314 representing NM_001201457 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCGGGCTGTTCGTCTTCCAGTCCCCTGTCCTGTTCAACTTGGTACCTTAAGAAATGACTCCCTGG AAGCTCAGCTTCATGAGTATGTCAAACAAGGGAACTATGTGAAAGTGAAGAAAATTCTTAAGAAAGGAAT TTATGTTGATGCAGTTAACTCCTTGGGCCAAACAGCACTTTTTGTTGCGGCGTTATTGGGCCTTAGGAAA TTCGTTGATGTTCTGGTGGATTATGGATCAGATCCAAATCACCGCTGCTTTGATGGGAGCACCCCTGTCC ATGCAGCAGCATTTTCGGGCAATCAGTGGATCCTTAGCAAACTGCTGGATGCAGGAGGTGACCTGCGACT CCACGATGAGAGGGGTCAAAACCCGAAGACTTGGGCTTTGACAGCAGGAAAGGAGCGTAGCACCCAGATA GTGGAGTTCATGCAGCGCTGTGCCTCACACATGCAGGCCATCATCCAGGGCTTCTCTTACGACCTCCTGA AGAAGATAGACTCCCCGCAGCGGCTTGTCTACAGCCCGTCCTGGTGTGGGGGCCTCGTGCAGGGAAACCC TAATGGCTCTCCTAACCGACTGCTTAAAGCTGGAGTCATTTCTGCTCAAAATATCTACAGCTTTGGTTTT GGGAAGGCTATGCCTTGGTTTCAGTTTTATCTTACTGGGGCGACACAGATGGCCTATCTAGGATCTCTTC CGGTCATTGGAGAAAAGGAAGTGATTCAAGCTGATGATGAGCCCACCTTCTCTTTCTTCAGCGGCCCCTA CATGGTCATGACCAACCTAGTGTGGAATGGGAGCAGGGTCACAGTGAAAGAGCTGAATCTCCCCACCCAC CCACACTGCAGCAGGCTGCGGCTGGCCGACTTGTTAATTGCCGAGCAGGAACACAGCAGCAAGCTGCGGC ACCCCTACTTGCTACAGTTGATGGCTGTGTGTCTCTCCCAGGACCTAGAGAAAACCCGCCTTGTGTACGA GCGCATCACTATCGGCACATTGTTCAGTGTCCTTCATGAACGACGGTCCCAGTTCCCAGTGCTGCACATG GAGGTGATTGTGCACCTGCTGCTCCAGATATCTGATGCCCTGAGATACCTGCATTTCCAGGGGTTTATCC ACCGCTCCCTCAGCTCCTATGCTGTCCATATCATCTCCCCAGGTGAAGCGAGGCTGACCAACCTGGAGTA CATGTTGGAAAGCGAGGACAGAGGTGTACAGAGGGACCTGACTCGAGTGCCCCTTCCTACGCAGCTATAC AACTGGGCCGCACCAGAAGTGATCTTACAGAAGGCAGCCACAGTGAAATCAGACATCTACAGCTTTTCTA TGATCATGCAGGAGATTTTAACAGATGACATACCCTGGAAGGGCTTAGATGGCTCAGTTGTTAAAAAAGC CGTAGTCTCGGGGAATTATTTAGAAGCTGATGTCAGGCTTCCGAAACCTTACTATGATATTGTTAAGTCA GGCATCCACGTCAAGCAGAAAGACCGAACTATGAACCTTCAAGATATCCGGTATATTCTGAAGAATGACT TAAAGGATTTTACTGGAGCCCAGAGAACTCAACCAACCGAGAGCCCCAGAGTGCAGAGATACGGACTCCA TCCCGATGTCAATGTCTATCTAGGACTGACTTCAGAACACCCCAGAGAGACACCTGACATGGAAATCATA GAACTAAAGGAAATGGGCAGTCAACCTCATTCACCAAGGGTTCACTCTTTATTCACTGAGGGGACACTAG ATCCTCAGGCCCCAGATCCATGTCTGATGGCCAGGGAGACTCAGAATCAAGATGCTCCTTGCCCTGCTCC ATTTATGGCAGAAGAGGCCAGCAGCCCCAGCACAGGTCAGCCAAGCCTCTGCAGTTTCGAAATCAACGAG ATCTACTCAGGCTGCTTGATTTTGGAAGATGACATAGAAGAGCCTCCAGGAGCTGCTTCATCTTTGGAGG CAGACGGACCTAACCAGGTAGATGAACTGAAATCCATGGAAGAAGAGCTGGATAAGATGGAGAGAGAGGC GTGTTGTTTTGGCAGTGAGGATGAGAGCTCTTCAAAAGCTGAGACAGAGTACTCTTTTGATGACTGGGAC TGGCAAAACGGTTCACTCAGTTCACTCAGCCTTCCTGAGTCAACCAGAGAAGCCAAGAGCAATTTGAACA ACATGTCCACGACTGAGGAGTATCTCATCAGTAAGTGTGTGCTGGATCTAAAGATTATGCAGACAATAAT GCACGAGAATGATGATAGGCTGAGGAATATCGAGCAGATATTAGATGAAGTCGAGATGAAACAGAAGGAA CAGGAAGAGCGCATGTCTTTATGGGCCACTTCAAGAGAGTTTACAAATGCCTACAAGTTACCTCTGGCCG TGGGCCCTCCATCTTTAAACTATATTCCTCCTGTCCTACAGCTTTCAGGGGGTCAGAAGCCAGACACCAG TGGCAACTACCCAACCCTACCAAGATTTCCAAGAATGCTGCCGACTCTTTGTGACCCTGGAAAACAGAAC ACAGATGAACAATTTCAGTGCACTCAAGGAGCCAAGGACAGTTTGGAAACAAGCAGGATCCAAAATACCA GTAGCCAGGGAAGACCTAGAGAGTCCACTGCCCAAGCCAAAGCCACACAGTTTAATAGTGCACTCTTCAC TCTGTCAAGCCACCGGCAGGGACCTTCTGCATCACCCAGCTGTCACTGGGACTCTACCAGGATGAGTGTG GAACCTGTTTCTTCTGAAATCTATAATGCAGAGTCCAGAAATAAAGATGATGGAAAGGTACACTTAAAAT GGAAAATGGAGGTGAAAGAAATGGCAAAGAAAGCAGCTACTGGACAGCTCACAGTACCTCCTTGGCATCC TCAGAGTAGTCTGACTTTAGAGAGCGAGGCTGAAAATGAGCCCGACGCCCTGCTGCAGCCCCCCATTAGG AGCCCAGAAAACACGGATTGGCAGCGAGTTATTGAGTATCATAGGGAAAATGATGAGCCCAGAGGAAATG GCAAGTTTGACAAGACGGGCAACAATGACTGTGACAGTGACCAGCATGGCAGACAGCCCAGGCTTGGAAG CTTCACCAGTATCAGGCACCCATCTCCCAGACAAAAGGAGCAACCAGAGCATAGTGAAGCCTTCCAAGCA AGTTCTGACACATTGGTGGCTGTAGAGAAATCTTACAGTCATCAGTCCATGCAATCAACTTGTTCACCAG AGTCTTCTGAGGATATAACAGATGAATTTTTAACTCCAGACGGTGAATATTTTTACTCCTCGACTGCTCA AGAAAACTTAGCTCTAGAGACCTCGAGTCCCATAGAAGAGGACTTTGAAGGAATACAAGGTGCATTTGCC CAACCTCAAGTCTCTGGTGAGGAAAAGTTCCAAATGAGAAAAATTCTTGGAAAGAATGCTGAGATTTTGC CCAGGTCTCAATTTCAACCTGTACGAAGTACTGAAGATGAACAAGAAGAGACATCAAAGGAGTCACCAAA GGAACTGAAAGAGAAAGACATATCATTGACGGATATTCAAGACCTGTCTAGTATCTCCTATGAACCAGAC AGCTCTTTTAAGGAAGCTTCATGCAAAACACCCAAAATAAACCATGCACCTACCAGTGTCAGCACTCCAC TCAGCCCAGGGTCCGTTTCTTCAGCTGCCAGTCAGTATAAAGACTGCCTTGAAAGTATCACATTTCAGGT TAAGACAGAGTTTGCCTCTTGCTGGAACAGTCAAGAATTTATTCAAACTTTGTCTGATGACTTTATAAGT GTCCGAGAGAGAGCAAAGAAACTGGATTCTCTCCTTACTTCCTCTGAAACTCCCCCTTCAAGACTGACTG GTCTTAAAAGATTGTCTTCATTTATTGGGGCTGGATCCCCCAGCCTTGTTAAGGCATGTGACTCATCACC ACCCCATGCCACCCAGAGAAGGAGCCTGCCTAAAGTAGAAGCCTTCTCACAGCATCACATTGATGAGCTG CCACCACCATCTCAGGAGCTACTTGATGACATTGAGCTCTTGAAACAGCAGCAGGGCTCATCCACGGTGT TGCATGAGAACACAGCAAGTGATGGAGGAGGCACTGCAAATGATCAAAGGCACTTAGAAGAACAAGAAAC TGACAGTAAAAAAGAAGATAGTAGTATGCTTTTGTCCAAAGAAACTGAAGATCTTGGAGAGGACACAGAG AGAGCTCACTCTACTCTGGATGAGGACCTGGAAAGATGGCTGCAGCCACCTGAGGAGAGCGTGGAGCTAC AAGACCTTCCCAAGGGCTCTGAAAGGGAGACAAATATCAAAGATCAAAAAGTTGGTGAAGAGAAAAGAAA AAGGGAAGATAGCATTACACCAGAAAGAAGGAAATCAGAGGGTGTTCTAGGGACTTCTGAAGAAGATGAA CTAAAATCCTGTTTTTGGAAGCGACTAGGTTGGTCCGAATCATCCAGGATAATCGTGCTGGATCAGAGTG ACTTGTCAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235314 representing NM_001201457 Red=Cloning site Green=Tags(s) MSRAVRLPVPCPVQLGTLRNDSLEAQLHEYVKQGNYVKVKKILKKGIYVDAVNSLGQTALFVAALLGLRK FVDVLVDYGSDPNHRCFDGSTPVHAAAFSGNQWILSKLLDAGGDLRLHDERGQNPKTWALTAGKERSTQI VEFMQRCASHMQAIIQGFSYDLLKKIDSPQRLVYSPSWCGGLVQGNPNGSPNRLLKAGVISAQNIYSFGF GKAMPWFQFYLTGATQMAYLGSLPVIGEKEVIQADDEPTFSFFSGPYMVMTNLVWNGSRVTVKELNLPTH PHCSRLRLADLLIAEQEHSSKLRHPYLLQLMAVCLSQDLEKTRLVYERITIGTLFSVLHERRSQFPVLHM EVIVHLLLQISDALRYLHFQGFIHRSLSSYAVHIISPGEARLTNLEYMLESEDRGVQRDLTRVPLPTQLY NWAAPEVILQKAATVKSDIYSFSMIMQEILTDDIPWKGLDGSVVKKAVVSGNYLEADVRLPKPYYDIVKS GIHVKQKDRTMNLQDIRYILKNDLKDFTGAQRTQPTESPRVQRYGLHPDVNVYLGLTSEHPRETPDMEII ELKEMGSQPHSPRVHSLFTEGTLDPQAPDPCLMARETQNQDAPCPAPFMAEEASSPSTGQPSLCSFEINE IYSGCLILEDDIEEPPGAASSLEADGPNQVDELKSMEEELDKMEREACCFGSEDESSSKAETEYSFDDWD WQNGSLSSLSLPESTREAKSNLNNMSTTEEYLISKCVLDLKIMQTIMHENDDRLRNIEQILDEVEMKQKE QEERMSLWATSREFTNAYKLPLAVGPPSLNYIPPVLQLSGGQKPDTSGNYPTLPRFPRMLPTLCDPGKQN TDEQFQCTQGAKDSLETSRIQNTSSQGRPRESTAQAKATQFNSALFTLSSHRQGPSASPSCHWDSTRMSV EPVSSEIYNAESRNKDDGKVHLKWKMEVKEMAKKAATGQLTVPPWHPQSSLTLESEAENEPDALLQPPIR SPENTDWQRVIEYHRENDEPRGNGKFDKTGNNDCDSDQHGRQPRLGSFTSIRHPSPRQKEQPEHSEAFQA SSDTLVAVEKSYSHQSMQSTCSPESSEDITDEFLTPDGEYFYSSTAQENLALETSSPIEEDFEGIQGAFA QPQVSGEEKFQMRKILGKNAEILPRSQFQPVRSTEDEQEETSKESPKELKEKDISLTDIQDLSSISYEPD SSFKEASCKTPKINHAPTSVSTPLSPGSVSSAASQYKDCLESITFQVKTEFASCWNSQEFIQTLSDDFIS VRERAKKLDSLLTSSETPPSRLTGLKRLSSFIGAGSPSLVKACDSSPPHATQRRSLPKVEAFSQHHIDEL PPPSQELLDDIELLKQQQGSSTVLHENTASDGGGTANDQRHLEEQETDSKKEDSSMLLSKETEDLGEDTE RAHSTLDEDLERWLQPPEESVELQDLPKGSERETNIKDQKVGEEKRKREDSITPERRKSEGVLGTSEEDE LKSCFWKRLGWSESSRIIVLDQSDLSD TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001201457
ORF Size	4491 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001201457.2
RefSeq Size	4954 bp
RefSeq ORF	4494 bp
Locus ID	56155
UniProt ID	Q8IWB6
Protein Families	Protein Kinase
MW	168.4 kDa
Gene Summary	The protein encoded by this gene is necessary for intercellular bridges in germ cells, which are required for spermatogenesis. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]

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