STAC3 (NM_001286257) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MYPBB; NAM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC236311 representing NM_001286257 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACAAGGAACGGAAGAAGGGACAGGCAGATAAGAAAAATCCTGTAGCAGCCATGATGGAGGAGG AGCCAGAGTCGGCCAGACCAGAGGAAGGCAAACCCCAGGATGGAAACCCTGAAGGGGATAAGAAGGCTGA GAAGAAGACACCTGATGACAAGCACAAGCAGCCTGGCTTCCAGCAGTCTCATTACTTTGTGGCTCTCTAT CGGTTCAAAGCCCTGGAGAAGGACGATCTGGATTTCCCGCCAGGAGAGAAGATCACAGTCATTGATGACT CCAATGAAGAATGGTGGCGGGGGAAAATCGGGGAGAAGGTCGGATTTTTCCCTCCAAACTTCATCATTCG GGTCCGGGCTGGAGAACGTGTGCACCGCGTGACGAGATCCTTCGTGGGGAACCGCGAGATAGGGCAGATC ACTCTCAAGAAGGACCAGATCGTGGTGCAGAAAGGAGACGAAGCGGGCGGCTACGTCAAGGTCTACACCG GCCGCAAGGTGGGGCTGTTTCCCACCGACTTTCTAGAGGAAATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC236311 representing NM_001286257 Red=Cloning site Green=Tags(s) MANKERKKGQADKKNPVAAMMEEEPESARPEEGKPQDGNPEGDKKAEKKTPDDKHKQPGFQQSHYFVALY RFKALEKDDLDFPPGEKITVIDDSNEEWWRGKIGEKVGFFPPNFIIRVRAGERVHRVTRSFVGNREIGQI TLKKDQIVVQKGDEAGGYVKVYTGRKVGLFPTDFLEEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286257
ORF Size	534 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286257.2
RefSeq Size	1235 bp
RefSeq ORF	537 bp
Locus ID	246329
UniProt ID	Q96MF2
MW	20.8 kDa
Gene Summary	The protein encoded by this gene is a component of the excitation-contraction coupling machinery of muscles. This protein is a member of the Stac gene family and contains an N-terminal cysteine-rich domain and two SH3 domains. Mutations in this gene are a cause of Native American myopathy. [provided by RefSeq, Nov 2013]

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