METTL9 (NM_001288659) Human Tagged ORF Clone
CAT#: RC237119
- TrueORF®
METTL9 (myc-DDK-tagged) - Human methyltransferase like 9 (METTL9), transcript variant 3
ORF Plasmid: tGFP
"NM_001288659" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CGI-81; DREV; DREV1; PAP1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237119 representing NM_001288659
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGAAAGACCACGAGCTGGGATACCAAGTTCTATGAAAAGTGGTATGTGTGCAACAGAGAGAAAT TATGCGAATCACTCCAGGCTGTCTTTGTTCAGAGTTACCTTGATCAAGGAACACAGATCTTCTTAAACAA CAGCATTGAGAAATCGGGCTGGCTATTTATCCAATTATATCATTCTTTTGTGTCATCTGTTTTTAGCCTG TTTATGTCTAGAACATCTATCAATGGGTTGCTAGGAAGAGGCTCAATGTTTGTGTTTTCACCAGATCAGT TTCAGAGACTGCTTAAAATTAATCCAGACTGGAAAACCCACAGACTTCTTGATTTAGGTGCTGGAGATGG AGAAGTCACAAAAATCATGAGCCCTCATTTTGAAGAAATCTATGCCACTGAGCTTTCTGAAACTATGATA TGGCAGCTTCAGAAAAAGAAATACAGAGTCCTTGGTATAAATGAATGGCAGAATACGGGGTTCCAGTATG ATGTCATCAGCTGCCTGAACTTGCTGGACCGCTGTGATCAGCCCCTGACTTTGTTAAAAGATATCAGAAG TGTCTTGGAGCCAACTAGAGGCAGGGTCATCCTTGCCCTTGTCCTCCCCTTTCATCCCTATGTGGAAAAC GTAGGTGGCAAGTGGGAGAAACCATCAGAAATTTTGGAAATCAAAGGACAGAACTGGGAAGAACAAGTGA ATAGTCTGCCTGAAGTTTTCAGAAAAGCTGGTTTTGTTATCGAAGCTTTCACCAGACTACCATACCTGTG TGAAGGCGACATGTATAATGACTACTACGTTCTGGATGACGCTGTCTTTGTTCTCAAACCAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237119 representing NM_001288659
Red=Cloning site Green=Tags(s) MDRKTTSWDTKFYEKWYVCNREKLCESLQAVFVQSYLDQGTQIFLNNSIEKSGWLFIQLYHSFVSSVFSL FMSRTSINGLLGRGSMFVFSPDQFQRLLKINPDWKTHRLLDLGAGDGEVTKIMSPHFEEIYATELSETMI WQLQKKKYRVLGINEWQNTGFQYDVISCLNLLDRCDQPLTLLKDIRSVLEPTRGRVILALVLPFHPYVEN VGGKWEKPSEILEIKGQNWEEQVNSLPEVFRKAGFVIEAFTRLPYLCEGDMYNDYYVLDDAVFVLKPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001288659 |
ORF Size | 834 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001288659.1, NP_001275588.1 |
RefSeq Size | 2924 bp |
RefSeq ORF | 837 bp |
Locus ID | 51108 |
UniProt ID | Q9H1A3 |
MW | 32.9 kDa |
Gene Summary | Protein-histidine N-methyltransferase that specifically catalyzes 1-methylhistidine (pros-methylhistidine) methylation of target proteins (PubMed:33563959). Mediates methylation of proteins with a His-x-His (HxH) motif (where 'x' is preferably a small amino acid) (PubMed:33563959). Catalyzes methylation of target proteins such as S100A9, NDUFB3, SLC39A5, SLC39A7, ARMC6 and DNAJB12; 1-methylhistidine modification may affect the binding of zinc and other metals to its target proteins (PubMed:33563959). Constitutes the main methyltransferase for the 1-methylhistidine modification in cell (PubMed:33563959).[UniProtKB/Swiss-Prot Function] |
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