STAC3 (NM_001286256) Human Tagged ORF Clone
CAT#: RC237449
- TrueORF®
STAC3 (myc-DDK-tagged) - Human SH3 and cysteine rich domain 3 (STAC3), transcript variant 2
ORF Plasmid: tGFP
"NM_001286256" in other vectors (2)
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CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MYPBB; NAM |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237449 representing NM_001286256
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAACTTCCCCCAGAGCCCCAGGCCAATGGGGAGGCAGTGGGAGCTGGGGGTGGGCCCATCTACTACA TCTATGAGGAAGAGGAAGAGGAAGAAGAGGAGGAGGAGGAGCCACCCCCAGAACCTCCTAAGCTGGTCAA CGATAAGCCCCACAAATTCAAAGATCACTTCTTCAAGAAGCCAAAGTTCTGTGATGTCTGTGCCCGGATG ATTGTTCTCAACAACAAGTTTGGGCTTCGCTGTAAGAACTGCAAAACCAACATCCATGAACACTGTCAGT CCTATGTGGAAATGCAGAGATGCTTCGGCAAGATCCCACCTGGTTTCCATCGGGCCTATAGTTCCCCACT CTACAGCAACCAGCAGTACGCTTGTGTCAAAGATCTCTCTGCTGCCAATCGCAATGATCCTGTGTTTGAA ACCCTGCGCACTGGGGTGATCATGGCAAACAAGGAACGGAAGAAGGGACAGGCAGATAAGAAAAATCCTG TAGCAGCCATGATGGAGGAGGAGCCAGAGTCGGCCAGACCAGAGGAAGGCAAACCCCAGGATGGAAACCC TGAAGGGGATAAGAAGGCTGAGAAGAAGACACCTGATGACAAGCACAAGCAGCCTGGCTTCCAGCAGTCT CATTACTTTGTGGCTCTCTATCGGTTCAAAGCCCTGGAGAAGGACGATCTGGATTTCCCGCCAGGAGAGA AGATCACAGTCATTGATGACTCCAATGAAGAATGGTGGCGGGGGAAAATCGGGGAGAAGGTCGGATTTTT CCCTCCAAACTTCATCATTCGGGTCCGGGCTGGAGAACGTGTGCACCGCGTGACGAGATCCTTCGTGGGG AACCGCGAGATAGGGCAGATCACTCTCAAGAAGGACCAGATCGTGGTGCAGAAAGGAGACGAAGCGGGCG GCTACGTCAAGGTCTACACCGGCCGCAAGGTGGGGCTGTTTCCCACCGACTTTCTAGAGGAAATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237449 representing NM_001286256
Red=Cloning site Green=Tags(s) MELPPEPQANGEAVGAGGGPIYYIYEEEEEEEEEEEEPPPEPPKLVNDKPHKFKDHFFKKPKFCDVCARM IVLNNKFGLRCKNCKTNIHEHCQSYVEMQRCFGKIPPGFHRAYSSPLYSNQQYACVKDLSAANRNDPVFE TLRTGVIMANKERKKGQADKKNPVAAMMEEEPESARPEEGKPQDGNPEGDKKAEKKTPDDKHKQPGFQQS HYFVALYRFKALEKDDLDFPPGEKITVIDDSNEEWWRGKIGEKVGFFPPNFIIRVRAGERVHRVTRSFVG NREIGQITLKKDQIVVQKGDEAGGYVKVYTGRKVGLFPTDFLEEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001286256 |
ORF Size | 975 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001286256.2 |
RefSeq Size | 1601 bp |
RefSeq ORF | 978 bp |
Locus ID | 246329 |
UniProt ID | Q96MF2 |
MW | 37.6 kDa |
Gene Summary | The protein encoded by this gene is a component of the excitation-contraction coupling machinery of muscles. This protein is a member of the Stac gene family and contains an N-terminal cysteine-rich domain and two SH3 domains. Mutations in this gene are a cause of Native American myopathy. [provided by RefSeq, Nov 2013] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Duplex signaling by CaM and Stac3 enhances CaV1.1 function and provides insights into congenital myopathy
,Niu, J;Yang, W;Yue, DT;Inoue, T;Ben-Johny, M;,
J. Gen. Physiol.
,PubMed ID 29950399
[STAC3]
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