SLC36A4 (NM_001286139) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PAT4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237770 representing NM_001286139 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTGAGTCCTTGGAGTTGTCTTCAGAAGCAAGCAGCATGGGGGCGGAGTGTGGTTGACTTTTTTC TGGTGATAACACAGCTGGGATTCTGTAGTGTTTATATTGTCTTCTTAGCTGAAAATGTGAAACAAGTTCA TGAAGGATTCCTGGAGAGTAAAGTGTTTATTTCAAATAGTACCAATTCATCAAACCCTTGTGAGAGAAGA AGTGTTGACCTAAGGATATATATGCTTTGCTTTCTTCCATTTATAATTCTTTTGGTCTTCATTCGTGAAC TAAAGAATCTATTTGTACTTTCATTCCTTGCCAACGTTTCCATGGCTGTCAGTCTTGTGATAATTTACCA GTATGTTGTCAGGAACATGCCAGATCCCCACAACCTTCCAATAGTGGCTGGTTGGAAGAAATACCCACTC TTTTTTGGTACTGCTGTATTTGCTTTTGAAGGCATAGGAGTGGTCCTTCCACTGGAAAACCAAATGAAAG AATCAAAGCGTTTCCCTCAAGCGTTGAATATTGGCATGGGGATTGTTACAACTTTGTATGTAACATTAGC TACTTTAGGATATATGTGTTTCCATGATGAAATCAAAGGCAGCATAACTTTAAATCTTCCCCAAGATGTA TGGTTATATCAATCAGTGAAAATTCTATATTCCTTTGGCATTTTTGTGACATATTCAATTCAGTTCTATG TTCCAGCAGAGATCATTATCCCTGGGATCACATCCAAATTTCATACTAAATGGAAGCAAATCTGTGAATT TGGGATAAGATCCTTCTTGGTTAGTATTACTTGTGCCGGAGCAATTCTTATTCCTCGTTTAGACATTGTG ATTTCCTTCGTTGGAGCTGTGAGCAGCAGCACATTGGCCCTAATCCTGCCACCTTTGGTTGAAATTCTTA CATTTTCGAAGGAACATTATAATATATGGATGGTCCTGAAAAATATTTCTATAGCATTCACTGGAGTTGT TGGCTTCTTATTAGGTACATATATAACTGTTGAAGAAATTATTTATCCTACTCCCAAAGTTGTAGCTGGC ACTCCACAGAGTCCTTTTCTAAATTTGAATTCAACATGCTTAACATCTGGTTTGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237770 representing NM_001286139 Red=Cloning site Green=Tags(s) MEVSPWSCLQKQAAWGRSVVDFFLVITQLGFCSVYIVFLAENVKQVHEGFLESKVFISNSTNSSNPCERR SVDLRIYMLCFLPFIILLVFIRELKNLFVLSFLANVSMAVSLVIIYQYVVRNMPDPHNLPIVAGWKKYPL FFGTAVFAFEGIGVVLPLENQMKESKRFPQALNIGMGIVTTLYVTLATLGYMCFHDEIKGSITLNLPQDV WLYQSVKILYSFGIFVTYSIQFYVPAEIIIPGITSKFHTKWKQICEFGIRSFLVSITCAGAILIPRLDIV ISFVGAVSSSTLALILPPLVEILTFSKEHYNIWMVLKNISIAFTGVVGFLLGTYITVEEIIYPTPKVVAG TPQSPFLNLNSTCLTSGLK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286139
ORF Size	1107 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286139.2
RefSeq Size	6061 bp
RefSeq ORF	1110 bp
Locus ID	120103
UniProt ID	Q6YBV0
Protein Families	Transmembrane
MW	41.8 kDa
Gene Summary	SLC36A4 belongs to the SLC36 family of amino acid transporters based on sequence similarity with other family members (e.g., SLC36A1; MIM 606561). SLC36 proteins contain about 500 amino acids and have 9 to 11 transmembrane domains. Unlike other SLC36 family members, which are proton-coupled amino acid transporters, SLC36A4 is a high-affinity/low-capacity non-proton-coupled amino acid transporter (Pillai and Meredith, 2011 [PubMed 21097500]).[supplied by OMIM, Feb 2011]

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