HSPBP1 (NM_001297600) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	FES1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238014 representing NM_001297600 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGGTCGCCGAGCTCCCCTGAAACGCCGCCTTCTCAGCAGACCCTCAAGAATCGACCCATCAGGAC GCCAGAGCTGCTTCAGCGGTGACCACCTTCTCCCTCTAACACATTCTTCCCTTCTTCACAAACGGCCCAT GTCAGACGAAGGCTCAAGGGGGAGCCGCCTGCCCCTGGCGCTGCCCCCGGCCTCCCAGGGTTGCTCTTCA GGGGGCGGCGGCGGCGGCTCCTCGGCTGGGGGCTCGGGCAATTCCCGGCCCCCACGCAACCTCCAAGGCT TGCTGCAGATGGCCATCACCGCGGGCTCTGAAGAGCCAGACCCTCCTCCAGAACCGATGAGTGAGGAGAG GCGTCAGTGGCTGCAGGAGGCCATGTCGGCTGCCTTCCGAGGCCAGCGGGAGGAGGTGGAGCAGATGAAG AGCTGCCTCCGAGTGCTGTCACAGCCCATGCCCCCCACTGCTGGGGAGGCCGAGCAGGCGGCCGACCAGC AAGAGCGAGAGGGGGCCCTGGAGCTGCTGGCCGACCTGTGTGAGAACATGGACAATGCCGCAGACTTCTG CCAGCTGTCTGGCATGCACCTGCTGGTGGGCCGGTACCTGGAGGCGGGGGCTGCGGGACTGCGGTGGCGG GCGGCACAGCTCATCGGCACGTGCAGTCAGAACGTGGCAGCCATCCAGGAGCAGGTGCTGGGCCTGGGTG CCCTGCGTAAGCTGCTGCGGCTGCTGGACCGCGACGCCTGCGACACGGTGCGCGTCAAGGCCCTCTTCGC CATCTCCTGTCTGGTCCGAGAGCAGGAGGCTGGGCTGCTGCAGTTCCTCCGCCTGGACGGCTTCTCTGTG TTGATGAGGGCCATGCAGCAGCAGGTGCAGAAGCTCAAGGTCAAATCAGCATTCCTGCTGCAGAACCTGC TGGTGGGCCACCCTGAACACAAAGGGACCCTGTGCTCCATGGGGATGGTCCAGCAGCTGGTGGCCCTGGT GCGGACAGAGCACAGCCCCTTCCACGAGCACGTGCTTGGAGCCCTGTGCAGCCTGGTGACAGACTTTCCG CAGGGTGTGCGCGAGTGTCGGGAGCCGGAACTGGGCCTGGAGGAGCTCCTCCGCCACCGCTGTCAGCTGC TGCAGCAGCATGAGGAGTACCAGGAGGAGCTGGAGTTCTGTGAAAAGCTGCTACAGACCTGTTTCTCCAG CCCAGCGGACGACAGCATGGATCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238014 representing NM_001297600 Red=Cloning site Green=Tags(s) MGGRRAPLKRRLLSRPSRIDPSGRQSCFSGDHLLPLTHSSLLHKRPMSDEGSRGSRLPLALPPASQGCSS GGGGGGSSAGGSGNSRPPRNLQGLLQMAITAGSEEPDPPPEPMSEERRQWLQEAMSAAFRGQREEVEQMK SCLRVLSQPMPPTAGEAEQAADQQEREGALELLADLCENMDNAADFCQLSGMHLLVGRYLEAGAAGLRWR AAQLIGTCSQNVAAIQEQVLGLGALRKLLRLLDRDACDTVRVKALFAISCLVREQEAGLLQFLRLDGFSV LMRAMQQQVQKLKVKSAFLLQNLLVGHPEHKGTLCSMGMVQQLVALVRTEHSPFHEHVLGALCSLVTDFP QGVRECREPELGLEELLRHRCQLLQQHEEYQEELEFCEKLLQTCFSSPADDSMDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297600
ORF Size	1215 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297600.2
RefSeq Size	1715 bp
RefSeq ORF	1218 bp
Locus ID	23640
UniProt ID	Q9NZL4
MW	44.9 kDa
Gene Summary	Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.[UniProtKB/Swiss-Prot Function]

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