TENT2 (NM_001297744) Human Tagged ORF Clone
CAT#: RC238475
- TrueORF®
PAPD4 (myc-DDK-tagged) - Human PAP associated domain containing 4 (PAPD4), transcript variant 4
ORF Plasmid: tGFP
"NM_001297744" in other vectors (2)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | APD4; GLD2; PAPD4; TUT2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC238475 representing NM_001297744
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCAAACTCAATTTTGGGTCGCCCACCCTTCACTCCAAATCATCAACAACATAATAACTTCTTTA CCCTGTCACCTACTGTTTATTCACACCAGCAGCTTATAGATGCACAATTCAACTTTCAGAATGCAGACTT GTCTAGAGCTGTGTCATTACAGCAGCTGACATATGGAAATGTCAGTCCAATACAGACCTCAGCTTCCCCA TTATTTCGAGGAAGGAAGAGATTAAGCGATGAAAAAAACCTTCCTCTTGACGGTAAACGGCAACGTTTCC ATTCACCCCACCAAGAGCCAACTGTAGTTAACCAGATAGTGCCTTTATCAGGTGAACGAAGATACTCAAT GCCACCATTGTTTCATACACATTATGTACCAGATATAGTCAGATGTGTTCCACCTTTTCGAGAAATTGCA TTTTTAGAACCTAGAGAAATCACACTGCCTGAGGCCAAAGATAAGTTGAGTCAGCAGATACTGGAGTTAT TTGAAACATGTCAGCAGCAAATAAGTGATTTAAAGAAGAAAGAACTCTGTCGAACACAGCTGCAGAGAGA AATTCAGCTGTTATTTCCACAAAGCAGACTTTTTTTGGTTGGGTCCTCTTTAAATGGATTTGGTACCCGG AGCAGTGATGGTGATTTATGCCTAGTTGTTAAGGAAGAACCAGTAAATCAGAAGACTGAAGCACGGCATA TACTCACCTTAGTCCATAAACACTTCTGTACTAGACTTTCGGGCTACATTGAGAGACCTCAGCTGATTCG AGCAAAAGTGCCAATTGTGAAGTTCAGGGATAAAGTCAGTTGTGTGGAGTTTGACTTGAATGTAAACAAT ATTGTTGGAATAAGAAACACATTCCTTCTCAGAACTTATGCATACCTTGAAAATCGAGTTCGTCCGTTAG TGCTGGTGATTAAGAAGTGGGCAAGTCACCATCAGATAAATGATGCCAGTCGTGGTACTTTAAGCAGCTA TAGTCTTGTATTGATGGTTTTGCACTATTTACAAACCCTACCTGAACCCATCCTTCCATCCCTCCAAAAA ATTTACCCAGAGTCTTTTAGTCCTGCTATACAGCTGCACCTTGTACATCAAGCTCCATGTAATGTTCCTC CTTACCTCTCAAAGAATGAATCAAACCTTGGGGACCTCTTACTGGGCTTTCTTAAATATTATGCTACAGA ATTTGACTGGAATAGTCAAATGATTTCAGTTCGTGAAGCCAAAGCCATTCCAAGGCCTGATGGTATTGAA TGGAGAAATAAATACATCTGTGTAGAAGAACCTTTTGATGGAACAAATACAGCCAGAGCAGTGCACGAAA AGCAGAAATTTGATATGATCAAGGATCAATTTTTAAAGTCATGGCACAGATTGAAAAACAAGAGAGATTT GAACAGTATACTACCTGTAAGAGCTGCTGTCCTGAAAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC238475 representing NM_001297744
Red=Cloning site Green=Tags(s) MFPNSILGRPPFTPNHQQHNNFFTLSPTVYSHQQLIDAQFNFQNADLSRAVSLQQLTYGNVSPIQTSASP LFRGRKRLSDEKNLPLDGKRQRFHSPHQEPTVVNQIVPLSGERRYSMPPLFHTHYVPDIVRCVPPFREIA FLEPREITLPEAKDKLSQQILELFETCQQQISDLKKKELCRTQLQREIQLLFPQSRLFLVGSSLNGFGTR SSDGDLCLVVKEEPVNQKTEARHILTLVHKHFCTRLSGYIERPQLIRAKVPIVKFRDKVSCVEFDLNVNN IVGIRNTFLLRTYAYLENRVRPLVLVIKKWASHHQINDASRGTLSSYSLVLMVLHYLQTLPEPILPSLQK IYPESFSPAIQLHLVHQAPCNVPPYLSKNESNLGDLLLGFLKYYATEFDWNSQMISVREAKAIPRPDGIE WRNKYICVEEPFDGTNTARAVHEKQKFDMIKDQFLKSWHRLKNKRDLNSILPVRAAVLKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001297744 |
ORF Size | 1440 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001297744.3 |
RefSeq Size | 3239 bp |
RefSeq ORF | 1443 bp |
Locus ID | 167153 |
UniProt ID | Q6PIY7 |
MW | 56 kDa |
Gene Summary | Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs (PubMed:15070731). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Adds a single nucleotide to the 3' end of specific miRNAs, monoadenylation stabilizes and prolongs the activity of some but not all miRNAs (PubMed:23200856).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |