TENT2 (NM_001297744) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APD4; GLD2; PAPD4; TUT2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336369 representing NM_001297744. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTCCCAAACTCAATTTTGGGTCGCCCACCCTTCACTCCAAATCATCAACAACATAATAACTTCTTT ACCCTGTCACCTACTGTTTATTCACACCAGCAGCTTATAGATGCACAATTCAACTTTCAGAATGCAGAC TTGTCTAGAGCTGTGTCATTACAGCAGCTGACATATGGAAATGTCAGTCCAATACAGACCTCAGCTTCC CCATTATTTCGAGGAAGGAAGAGATTAAGCGATGAAAAAAACCTTCCTCTTGACGGTAAACGGCAACGT TTCCATTCACCCCACCAAGAGCCAACTGTAGTTAACCAGATAGTGCCTTTATCAGGTGAACGAAGATAC TCAATGCCACCATTGTTTCATACACATTATGTACCAGATATAGTCAGATGTGTTCCACCTTTTCGAGAA ATTGCATTTTTAGAACCTAGAGAAATCACACTGCCTGAGGCCAAAGATAAGTTGAGTCAGCAGATACTG GAGTTATTTGAAACATGTCAGCAGCAAATAAGTGATTTAAAGAAGAAAGAACTCTGTCGAACACAGCTG CAGAGAGAAATTCAGCTGTTATTTCCACAAAGCAGACTTTTTTTGGTTGGGTCCTCTTTAAATGGATTT GGTACCCGGAGCAGTGATGGTGATTTATGCCTAGTTGTTAAGGAAGAACCAGTAAATCAGAAGACTGAA GCACGGCATATACTCACCTTAGTCCATAAACACTTCTGTACTAGACTTTCGGGCTACATTGAGAGACCT CAGCTGATTCGAGCAAAAGTGCCAATTGTGAAGTTCAGGGATAAAGTCAGTTGTGTGGAGTTTGACTTG AATGTAAACAATATTGTTGGAATAAGAAACACATTCCTTCTCAGAACTTATGCATACCTTGAAAATCGA GTTCGTCCGTTAGTGCTGGTGATTAAGAAGTGGGCAAGTCACCATCAGATAAATGATGCCAGTCGTGGT ACTTTAAGCAGCTATAGTCTTGTATTGATGGTTTTGCACTATTTACAAACCCTACCTGAACCCATCCTT CCATCCCTCCAAAAAATTTACCCAGAGTCTTTTAGTCCTGCTATACAGCTGCACCTTGTACATCAAGCT CCATGTAATGTTCCTCCTTACCTCTCAAAGAATGAATCAAACCTTGGGGACCTCTTACTGGGCTTTCTT AAATATTATGCTACAGAATTTGACTGGAATAGTCAAATGATTTCAGTTCGTGAAGCCAAAGCCATTCCA AGGCCTGATGGTATTGAATGGAGAAATAAATACATCTGTGTAGAAGAACCTTTTGATGGAACAAATACA GCCAGAGCAGTGCACGAAAAGCAGAAATTTGATATGATCAAGGATCAATTTTTAAAGTCATGGCACAGA TTGAAAAACAAGAGAGATTTGAACAGTATACTACCTGTAAGAGCTGCTGTCCTGAAAAGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297744
Insert Size	1443 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297744.1
RefSeq Size	3239 bp
RefSeq ORF	1443 bp
Locus ID	167153
UniProt ID	Q6PIY7
MW	55.5 kDa
Gene Summary	Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs (PubMed:15070731). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Adds a single nucleotide to the 3' end of specific miRNAs, monoadenylation stabilizes and prolongs the activity of some but not all miRNAs (PubMed:23200856).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate in-frame splice site in the central coding region. The resulting isoform (2) has the same N- and C-termini, but is shorter than isoform 1. Variants 4 and 12 encode the same protein (isoform 2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.