PNLDC1 (NM_001271862) Human Tagged ORF Clone
CAT#: RC238745
- TrueORF®
PNLDC1 (myc-DDK-tagged) - Human poly(A)-specific ribonuclease (PARN)-like domain containing 1 (PNLDC1), transcript variant 1
ORF Plasmid: tGFP
"NM_001271862" in other vectors (2)
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CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HsPNLDC1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC238745 representing NM_001271862
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTGGGCGCCGACGAGTTCGAGGAGAGCCTCCCTCTGCTGCAGGAGCTCGTCCAGGAGGCCGACT TCGTGGGTCTGGACATAGAGTTCACGGGCCTTCGTTCTAACCTGTCTGGGCCCCAGCAGATCAGTCTTTT TGATTTGCCATCGGAGTGGTATCTAAAGACCCGTCAGAGTGTTCAGCAATTTACAGTCTGTCAGATTGGA TTGTCTGTGTTTTCCGCTATTGAAGGAGAGGCAAACAAGTATATAGCCCATTCTTGTAACTTCTATCTCT TCCCTACAACGTTTGGGATTTTGGACTCAGAATTCTCCTTCCAGGCTTCCAGTGTTCAGTTTTTGAATCA GTATGGCTTCAACTATAACAAGTTTCTCAAAAACGGAATCCCATATATGAATGAAGAACAGGAGAAGAAA ATTAGACACGATATCCTGACTGGGAACTGGAGAGTTCGCAGCTCTCCGGATAAAGACCAAATCAAGGTGG TGATTGACGAAGTGACGCGGTGGCTGGAGCTGGCCAAGGAAGGCGACTGGATGACTCTTCCTGGGATCAC TGGCTTCCAGGCCTTTGAGGTCCAACTGGTGCTGAGGCAGGCCCTCCCCAACATCTGGACGGTGCTGAAA GATGAGGGGGTGGTAGTGAAGAAAGTGAGTAAACAACATCGTTGGTATCTTCAGAACACCTCTTGTGACC GAGAGAGCTGTTGGAAGGAAAATATTCTTCTCTCAGCAAGGGGTTTTTCTGTCTTTTTCCAAATGCTGGT GAAAGCCCAGAAGCCCTTAGTGGGACATAATATGATGATGGACCTGCTGCACCTCCATGAGAAGTTCTTC AGACCCCTCCCAGAAAGCTACGATCAATTTAAGCAGAATATCCACAGCCTATTTCCTGTTCTCATTGATA CCAAGAGTGTAACAAAGGATATCTGGAAGGAGATGAATTTCCCGAGGGTGTCGAATCTTTCGGAAGTCTA TGAAGTCCTGAACAGTGACTTGAATCCCACCAAGAATTCTGGACCAGAGATTGTTCACGCGAGCAGGTGT GAGAAATATGTTGAGACAAAGTGCCCCCACGAAGCCGCGTATGATGCCTTCCTCTGTGGGTCAGTTCTTT TGAAAGTGGCACACTTGCTTCTACAGAAGATATACCACATCGACCCCGTGCCCGAGTCATCCTTTCCTCA GTACCTTGACGTGCTGGCTCCTTACGTGAACCAAGTGAACCTCATCCGAGCGGGGGTCCCAAAGATCAAT TTTTCTGGTCCAGATTATCCCAGTATCCGACCTCCCATCCTCATCCTCAGCGTCAAAAGGTGGCCTGGGG TCAGCGAGCAGCAAGTCTACCATAAGTTTCAGAATCTCTGCAAGTTTGATGTCAGGCGACTCACAAGAAG CCAGTTCTTACTCCTGACCAATAAGTTTAAGGATGCGCGGAACATCCTGAAGGAGTACCGGGACCACCCG ACCCTGTGCATCTCCCTGTACCGCTACTGGAGGCACTCCCCAAACGTCAACTGCCTGCTCCAAGTCTGTG GCATAGTGACTGCCTGGGCCCTTCTCGCGTTCATCCTTGGAAGATCTGGTACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC238745 representing NM_001271862
Red=Cloning site Green=Tags(s) MDVGADEFEESLPLLQELVQEADFVGLDIEFTGLRSNLSGPQQISLFDLPSEWYLKTRQSVQQFTVCQIG LSVFSAIEGEANKYIAHSCNFYLFPTTFGILDSEFSFQASSVQFLNQYGFNYNKFLKNGIPYMNEEQEKK IRHDILTGNWRVRSSPDKDQIKVVIDEVTRWLELAKEGDWMTLPGITGFQAFEVQLVLRQALPNIWTVLK DEGVVVKKVSKQHRWYLQNTSCDRESCWKENILLSARGFSVFFQMLVKAQKPLVGHNMMMDLLHLHEKFF RPLPESYDQFKQNIHSLFPVLIDTKSVTKDIWKEMNFPRVSNLSEVYEVLNSDLNPTKNSGPEIVHASRC EKYVETKCPHEAAYDAFLCGSVLLKVAHLLLQKIYHIDPVPESSFPQYLDVLAPYVNQVNLIRAGVPKIN FSGPDYPSIRPPILILSVKRWPGVSEQQVYHKFQNLCKFDVRRLTRSQFLLLTNKFKDARNILKEYRDHP TLCISLYRYWRHSPNVNCLLQVCGIVTAWALLAFILGRSGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001271862 |
ORF Size | 1593 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001271862.2 |
RefSeq Size | 1859 bp |
RefSeq ORF | 1596 bp |
Locus ID | 154197 |
UniProt ID | Q8NA58 |
MW | 61.8 kDa |
Gene Summary | 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails (PubMed:27515512). Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development (PubMed:27515512). May act as a regulator of multipotency in embryonic stem cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RG238745 | PNLDC1 (tGFP-tagged) - Human poly(A)-specific ribonuclease (PARN)-like domain containing 1 (PNLDC1), transcript variant 1 |
CNY 4,940.00 |
|
SC336639 | PNLDC1 (untagged) - Human poly(A)-specific ribonuclease (PARN)-like domain containing 1 (PNLDC1), transcript variant 1 |
CNY 5,130.00 |