MUC20 (NM_001291833) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MUC-20
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238777 representing NM_001291833 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGTCTCTGGGGTCTGGCTCTGCCCCTTTTCTTCTGCTGGGAGGTTGGGGTCTCTGGGAGCTCTG CAGGCCCCAGCACCCGCAGAGCAGACACTGCGATGACAACGGACGACACAGAAGTGCCCGCTATGACTCT AGCACCGGGCCACGCCGCTCTGGAAACTCAAACGCTGAGCGCTGAGACCTCTTCTAGGGCCTCAACCCCA GCCGGCCCCATTCCAGAAGCAGAGACCAGGGGAGCCAAGAGAATTTCCCCTGCAAGAGAGACCAGGAGTT TCACAAAAACATCTCCCAACTTCATGGTGCTGATCGCCACCTCCGTGGAGACATCAGCCGCCAGTGGCAG CCCCGAGGGAGCTGGAATGACCACAGTTCAGACCATCACAGGCAGTGATCCCGAGGAAGCCATCTTTGAC ACCCTTTGCACCGATGACATCTCTGAAGAGGCAAAGACACTCACAATGGACATATTGACATTGGCTCACA CCTCCACAGAAGCTAAGGGCCTGTCCTCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCAGTCATCAC CCCGTCACGGGCCTCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCAGTCATCACCCCGTCACGGGCC TCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCAGTCATCACCCCCTCATGGTCCCCGGGATCTGACG TCACTCTCCTCGCTGAAGCCCTGGTGACTGTCACAAACATCGAGGTTATTAATTGCAGCATCACAGAAAC AACAACTTCCAGCATCCCTGGGGCCTCAGACATAGATCTCATCCCCACGGAAGGGGTGAAGGCCTCGTCC ACCTCCGATCCACCAGCTCTGCCTGACTCCACTGAAGCAAAACCACACATCACTGAGGTCACAGCCTCTG CCGAGACCCTGTCCACAGCCGGCACCACAGAGTCAGCTGCACCTCATGCCACGGTTGGGACCCCACTCCC CACTAACAGCGCCACAGAAAGAGAAGTGACAGCACCCGGGGCCACGACCCTCAGTGGAGCTCTGGTCACA GTTAGCAGGAATCCCCTGGAAGAAACCTCAGCCCTCTCTGTTGAGACACCAAGTTACGTCAAAGTCTCAG GAGCAGCTCCGGTCTCCATAGAGGCTGGGTCAGCAGTGGGCAAAACAACTTCCTTTGCTGGGAGCTCTGC TTCCTCCTACAGCCCCTCGGAAGCCGCCCTCAAGAACTTCACCCCTTCAGAGACACCGACCATGGACATC GCAACCAAGGGGCCCTTCCCCACCAGCAGGGACCCTCTTCCTTCTGTCCCTCCGACTACAACCAACAGCA GCCGAGGGACGAACAGCACCTTAGCCAAGATCACAACCTCAGCGAAGACCACGATGAAGCCCCCAACAGC CACGCCCACGACTGCCCGGACGAGGCCGACCACAGACGTGAGTGCAGGTGAAAATGGAGGTTTCCTCCTC CTGTGGCTGAGTGTGGCTTCCCCGGAAGACCTCACTGACCCCAGAGTGGCAGAAAGGCTGATGCAGCAGC TCCACCGGGAACTCCACGCCCACGCGCCTCACTTCCAGGTCTCCTTACTGCGTGTCAGGAGAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238777 representing NM_001291833 Red=Cloning site Green=Tags(s) MGCLWGLALPLFFCWEVGVSGSSAGPSTRRADTAMTTDDTEVPAMTLAPGHAALETQTLSAETSSRASTP AGPIPEAETRGAKRISPARETRSFTKTSPNFMVLIATSVETSAASGSPEGAGMTTVQTITGSDPEEAIFD TLCTDDISEEAKTLTMDILTLAHTSTEAKGLSSESSASSDGPHPVITPSRASESSASSDGPHPVITPSRA SESSASSDGPHPVITPSWSPGSDVTLLAEALVTVTNIEVINCSITETTTSSIPGASDIDLIPTEGVKASS TSDPPALPDSTEAKPHITEVTASAETLSTAGTTESAAPHATVGTPLPTNSATEREVTAPGATTLSGALVT VSRNPLEETSALSVETPSYVKVSGAAPVSIEAGSAVGKTTSFAGSSASSYSPSEAALKNFTPSETPTMDI ATKGPFPTSRDPLPSVPPTTTNSSRGTNSTLAKITTSAKTTMKPPTATPTTARTRPTTDVSAGENGGFLL LWLSVASPEDLTDPRVAERLMQQLHRELHAHAPHFQVSLLRVRRG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291833
ORF Size	1605 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291833.1, NP_001278762.1
RefSeq Size	2086 bp
RefSeq ORF	1608 bp
Locus ID	200958
UniProt ID	Q8N307
MW	55.3 kDa
Gene Summary	This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014]

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