MUC20 (NM_001291833) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MUC-20
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336671 representing NM_001291833. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGCTGTCTCTGGGGTCTGGCTCTGCCCCTTTTCTTCTGCTGGGAGGTTGGGGTCTCTGGGAGCTCT GCAGGCCCCAGCACCCGCAGAGCAGACACTGCGATGACAACGGACGACACAGAAGTGCCCGCTATGACT CTAGCACCGGGCCACGCCGCTCTGGAAACTCAAACGCTGAGCGCTGAGACCTCTTCTAGGGCCTCAACC CCAGCCGGCCCCATTCCAGAAGCAGAGACCAGGGGAGCCAAGAGAATTTCCCCTGCAAGAGAGACCAGG AGTTTCACAAAAACATCTCCCAACTTCATGGTGCTGATCGCCACCTCCGTGGAGACATCAGCCGCCAGT GGCAGCCCCGAGGGAGCTGGAATGACCACAGTTCAGACCATCACAGGCAGTGATCCCGAGGAAGCCATC TTTGACACCCTTTGCACCGATGACATCTCTGAAGAGGCAAAGACACTCACAATGGACATATTGACATTG GCTCACACCTCCACAGAAGCTAAGGGCCTGTCCTCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCA GTCATCACCCCGTCACGGGCCTCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCAGTCATCACCCCG TCACGGGCCTCAGAGAGCAGCGCCTCTTCCGACGGCCCCCATCCAGTCATCACCCCCTCATGGTCCCCG GGATCTGACGTCACTCTCCTCGCTGAAGCCCTGGTGACTGTCACAAACATCGAGGTTATTAATTGCAGC ATCACAGAAACAACAACTTCCAGCATCCCTGGGGCCTCAGACATAGATCTCATCCCCACGGAAGGGGTG AAGGCCTCGTCCACCTCCGATCCACCAGCTCTGCCTGACTCCACTGAAGCAAAACCACACATCACTGAG GTCACAGCCTCTGCCGAGACCCTGTCCACAGCCGGCACCACAGAGTCAGCTGCACCTCATGCCACGGTT GGGACCCCACTCCCCACTAACAGCGCCACAGAAAGAGAAGTGACAGCACCCGGGGCCACGACCCTCAGT GGAGCTCTGGTCACAGTTAGCAGGAATCCCCTGGAAGAAACCTCAGCCCTCTCTGTTGAGACACCAAGT TACGTCAAAGTCTCAGGAGCAGCTCCGGTCTCCATAGAGGCTGGGTCAGCAGTGGGCAAAACAACTTCC TTTGCTGGGAGCTCTGCTTCCTCCTACAGCCCCTCGGAAGCCGCCCTCAAGAACTTCACCCCTTCAGAG ACACCGACCATGGACATCGCAACCAAGGGGCCCTTCCCCACCAGCAGGGACCCTCTTCCTTCTGTCCCT CCGACTACAACCAACAGCAGCCGAGGGACGAACAGCACCTTAGCCAAGATCACAACCTCAGCGAAGACC ACGATGAAGCCCCCAACAGCCACGCCCACGACTGCCCGGACGAGGCCGACCACAGACGTGAGTGCAGGT GAAAATGGAGGTTTCCTCCTCCTGTGGCTGAGTGTGGCTTCCCCGGAAGACCTCACTGACCCCAGAGTG GCAGAAAGGCTGATGCAGCAGCTCCACCGGGAACTCCACGCCCACGCGCCTCACTTCCAGGTCTCCTTA CTGCGTGTCAGGAGAGGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291833
Insert Size	1608 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291833.1
RefSeq Size	2086 bp
RefSeq ORF	1608 bp
Locus ID	200958
UniProt ID	Q8N307
MW	54.8 kDa
Gene Summary	This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins secreted by many epithelial tissues to form an insoluble mucous barrier. The C-terminus of this family member associates with the multifunctional docking site of the MET proto-oncogene and suppresses activation of some downstream MET signaling cascades. The protein features a mucin tandem repeat domain that varies between two and six copies in most individuals. Multiple variants encoding different isoforms have been found for this gene. A related pseudogene, which is also located on chromosome 3, has been identified. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (3) lacks a codon in the 5' coding region and nine mucin tandem repeat sequences in the central coding region, and also has additional polymorphisms in the coding region and UTRs, compared to variant 1. The encoded isoform (c) is shorter than isoform a. This variant includes three mucin tandem repeats and is represented on the ALT_REF_LOCI_6 alternate haplotype of the reference genome. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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