MARCHF8 (NM_001282866) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	c-MIR; CMIR; MARCH-VIII; MARCH8; MIR; RNF178
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238944 representing NM_001282866 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCATGCCACTGCATCAGATCTCTGCCATTCCATCCCAGGATGCCATCTCTGCTAGAGTCTACAGAA GTAAGACCAAAGAAAAGGAGAGGGAAGAACAGAATGAGAAGACTTTGGGACATTTCATGAGTCATTCAAG CAACATTTCTAAGGCTGGGAGTCCTCCGTCAGCATCAGCTCCGGCTCCGGTGTCCTCCTTCTCTCGCACT TCTATCACGCCATCCAGCCAGGACATCTGCAGTTCCAGTGCAGTGTTTTCTGAGTGTTGTCACCACAGTT CCGTGCAGTCTGCTGTTGTCTCGAAAGCTCCTCACTGCCAGAGTTCTCTGACACAAGGGCTCACTGTGAC AGTTATCTGTAAGGACACATTACAGGCGTCAAAGAGAAATTCCTTTGGTTCAGAATGGGCCCAGGCCTTG AAGCCTGCTAAGAATACCAAAGCCAGAAGAACACTAAAGTTCTCAAGGTCCCTCAATGATGTGGGTGAGA AGGCGCAGGATACTTCAGAAAGTTTTGCCTATGTGGAAAGAACTTGTTCTGAAGGGAAATTAATACTCCC TCAAGATACGTGTCTCAGAACTAACAGGTTTCATCATAAAGAAAAAAGAACCCTGAACCACAAACCTCTT GGCAATTCCAAACATTCTTGTGTTTCATGCCTTTCTGCCGGTCGCTCAACTGCCTCAGAGGTGGAAGCTG GCAAGGGGGGCAGGCCCGGCCTGCTGCTGGAAGAGAAGGCGGATGGTGAGGCCACGTCCCGAAGCCGGCA ACTGCTCCAGTACCTGTTCTCACTCTCGCACGGCTTGAGCGCCAGCAGCCTGCACAGGTTCCATGAGCTG GAGAGCTGCGCTGCTCGCCTGCACACTGCCAAGTCCTCCAGCGGGCTGGCAGGGAGTATGGGCTTCTGCT CTGACGAGATGGGAGACGACGATGTCTTTGAGGACAGCACATCTGCAAAACTGAAGAGTAGGGTTCTGCG GGCGCCCCTCTGCTCCACGGAAAAGGACAGCGACCTGGATTGTCCTTCTCCCTTCTCTGAAAAATTACCC CCCATATCTCCCGTGTCCACGTCAGGGGATGTCTGCAGGATCTGCCACTGTGAAGGAGATGATGAGAGCC CCCTGATCACCCCCTGCCACTGCACAGGAAGCCTCCACTTCGTGCACCAGGCCTGCCTGCAGCAGTGGAT CAAGAGCTCCGACACGCGCTGCTGCGAGCTCTGCAAGTATGAGTTCATCATGGAGACCAAGCTGAAGCCA CTGAGAAAATGGGAGAAGTTGCAGATGACGTCCAGCGAGCGCAGGAAGATCATGTGCTCAGTGACATTCC ACGTCATTGCCATCACATGTGTGGTCTGGTCCTTGTATGTGCTCATTGACCGTACTGCTGAGGAGATCAA GCAGGGGCAGGCAACAGGAATCCTAGAATGGCCCTTTTGGACTAAATTGGTGGTTGTGGCCATCGGCTTC ACCGGAGGACTTCTTTTTATGTATGTTCAGTGTAAAGTGTATGTGCAATTGTGGAAGAGACTCAAGGCCT ATAATAGAGTGATCTATGTTCAAAACTGTCCAGAAACAAGCAAAAAGAATATTTTTGAAAAATCTCCACT AACAGAGCCCAACTTTGAAAATAAACATGGATATGGAATCTGTCATTCCGACACAAACTCTTCTTGTTGC ACAGAGCCTGAAGACACTGGAGCAGAAATCATTCACGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238944 representing NM_001282866 Red=Cloning site Green=Tags(s) MSMPLHQISAIPSQDAISARVYRSKTKEKEREEQNEKTLGHFMSHSSNISKAGSPPSASAPAPVSSFSRT SITPSSQDICSSSAVFSECCHHSSVQSAVVSKAPHCQSSLTQGLTVTVICKDTLQASKRNSFGSEWAQAL KPAKNTKARRTLKFSRSLNDVGEKAQDTSESFAYVERTCSEGKLILPQDTCLRTNRFHHKEKRTLNHKPL GNSKHSCVSCLSAGRSTASEVEAGKGGRPGLLLEEKADGEATSRSRQLLQYLFSLSHGLSASSLHRFHEL ESCAARLHTAKSSSGLAGSMGFCSDEMGDDDVFEDSTSAKLKSRVLRAPLCSTEKDSDLDCPSPFSEKLP PISPVSTSGDVCRICHCEGDDESPLITPCHCTGSLHFVHQACLQQWIKSSDTRCCELCKYEFIMETKLKP LRKWEKLQMTSSERRKIMCSVTFHVIAITCVVWSLYVLIDRTAEEIKQGQATGILEWPFWTKLVVVAIGF TGGLLFMYVQCKVYVQLWKRLKAYNRVIYVQNCPETSKKNIFEKSPLTEPNFENKHGYGICHSDTNSSCC TEPEDTGAEIIHV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282866
ORF Size	1719 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282866.2
RefSeq Size	5658 bp
RefSeq ORF	1722 bp
Locus ID	220972
UniProt ID	Q5T0T0
Protein Families	Druggable Genome, Transmembrane
MW	63.8 kDa
Gene Summary	MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]

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