Myelin expression factor 2 (MYEF2) (NM_001301210) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HsT18564; MEF-2; MST156; MSTP156; myEF-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238958 representing NM_001301210 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGCCAACAAGGCCGAGGTGCCCGGGGCCACTGGTGGCGACAGCCCGCACCTGCAGCCCGCAG AGCCGCCGGGCGAGCCGCGGCGAGAGCCGCACCCCGCGGAGGCGGAGAAGCAGCAGCCGCAGCACAGCAG CAGCTCCAATGGCGTTAAAATGGAGAATGATGAATCAGCAAAAGAAGAGAAATCTGACTTAAAGGAAAAA TCTACAGGAAGTAAGAAGGCCAATAGATTTCATCCTTATTCAAAAGACAAGAATTCGGGCACTGGAGAAA AGAAGGGTCCAAATCGTAACAGAGTTTTCATTAGCAACATCCCATATGACATGAAATGGCAAGCTATTAA AGATCTAATGAGAGAGAAAGTTGGTGAGGTTACATACGTGGAGCTCTTTAAGGATGCGGAAGGAAAATCA AGGGGTTGTGGTGTGGTTGAATTCAAAGATGAAGAATTTGTAAAGAAAGCCCTAGAAACTATGAACAAAT ATGATCTTAGTGGAAGACCCCTTAATATTAAAGAGGATCCTGATGGAGAAAATGCTCGTAGGGCATTGCA GCGAACAGGAGGATCATTTCCAGGAGGACACGTCCCTGATATGGGATCAGGGTTGATGAATTTACCACCT TCCATACTCAATAATCCAAACATTCCTCCTGAAGTCATCAGTAATTTGCAGGCCGGTAGACTTGGTTCCA CAATTTTTGTTGCCAATCTTGACTTCAAAGTTGGTTGGAAGAAGCTAAAGGAAGTGTTCAGCATAGCTGG AACTGTGAAGCGGGCAGATATTAAAGAAGACAAAGATGGCAAGAGCAGAGGAATGGGCACTGTCACTTTT GAGCAAGCAATTGAAGCAGTTCAAGCAATTTCTATGTTCAATGGGCAGTTTTTATTTGATAGACCTATGC ATGTGAAAATGGATGACAAGTCTGTTCCTCATGAAGAGTACCGTTCACATGATGGTAAAACACCACAATT ACCACGTGGTCTTGGAGGCATTGGGATGGGACTTGGTCCGGGTGGACAGCCTATTAGTGCCAGCCAGTTG AACATAGGTGGAGTAATGGGAAATTTAGGTCCAGGTGGTATGGGAATGGATGGTCCAGGTTTTGGAGGAA TGAATAGAATTGGAGGAGGAATAGGGTTTGGTGGTCTGGAAGCAATGAATAGCATGGGAGGATTTGGAGG AGTTGGCCGAATGGGAGAGCTGTACCGTGGTGCGATGACTAGTAGCATGGAGCGAGATTTTGGACGTGGT GATATTGGAATAAATCGAGGCTTTGGAGATTCCTTTGGTAGACTTGGTGGTGGAATGGGTAGCATGAACA GTGTGACTGGAGGAATGGGGATGGGACTGGACCGGATGAGTTCCAGCTTTGATAGAATGGGACCAGGTAT AGGAGCTATACTGGAAAGGAGCATCGATATGGATCGAGGATTTTTATCGGGTCCAATGGGAAGCGGAATG AGAGAGAGAATAGGCTCCAAAGGCAACCAGATATTTGTCAGAAATCTACCTTTTGACTTGACTTGGCAGA AACTAAAAGAGAAATTCAGTCAGTGTGGTCATGTAATGTTTGCAGAAATAAAAATGGAGAATGGAAAGTC AAAAGGCTGTGGAACAGTCAGATTTGACTCCCCAGAATCAGCTGAAAAAGCCTGCAGAATAATGAATGGC ATAAAAATCAGTGGCAGAGAAATTGATGTTCGCTTGGATCGTAATGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238958 representing NM_001301210 Red=Cloning site Green=Tags(s) MADANKAEVPGATGGDSPHLQPAEPPGEPRREPHPAEAEKQQPQHSSSSNGVKMENDESAKEEKSDLKEK STGSKKANRFHPYSKDKNSGTGEKKGPNRNRVFISNIPYDMKWQAIKDLMREKVGEVTYVELFKDAEGKS RGCGVVEFKDEEFVKKALETMNKYDLSGRPLNIKEDPDGENARRALQRTGGSFPGGHVPDMGSGLMNLPP SILNNPNIPPEVISNLQAGRLGSTIFVANLDFKVGWKKLKEVFSIAGTVKRADIKEDKDGKSRGMGTVTF EQAIEAVQAISMFNGQFLFDRPMHVKMDDKSVPHEEYRSHDGKTPQLPRGLGGIGMGLGPGGQPISASQL NIGGVMGNLGPGGMGMDGPGFGGMNRIGGGIGFGGLEAMNSMGGFGGVGRMGELYRGAMTSSMERDFGRG DIGINRGFGDSFGRLGGGMGSMNSVTGGMGMGLDRMSSSFDRMGPGIGAILERSIDMDRGFLSGPMGSGM RERIGSKGNQIFVRNLPFDLTWQKLKEKFSQCGHVMFAEIKMENGKSKGCGTVRFDSPESAEKACRIMNG IKISGREIDVRLDRNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301210
ORF Size	1728 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301210.1, NP_001288139.1
RefSeq Size	5335 bp
RefSeq ORF	1731 bp
Locus ID	50804
UniProt ID	Q9P2K5
Protein Families	Transcription Factors
MW	62.4 kDa
Gene Summary	Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA (By similarity).[UniProtKB/Swiss-Prot Function]

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