AKAP8L (NM_001291478) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HA95; HAP95; NAKAP; NAKAP95
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC238988 representing NM_001291478 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTACACAGGCTTTGTCCAGGGATCTGAAACCACTTTGCAGTCGACATACTCGGATACCAGCGCTC AGCCCACCTGTGATTATGGATATGGAACTTGGAACTCTGGGACAAATAGAGGCTACGAGGGCTATGGCTA TGGCTATGGCTATGGCCAGGATAACACCACCAACTATGGGTATGACTCTTATGAGTCCTGCGACTCGAGG GCCGTCCTGAGTGAGCGCGACCTGTACCGGTCAGGCTATGACTACAGCGAGCTTGACCCTGAGATGGAAA TGGCCTATGAGGGCCAATACGATGCCTACCGCGACCAGTTCCGCATGCGTGGCAACGACACCTTCGGTCC CAGGGCACAGGGCTGGGCCCGGGATGCCCGGAGCGGCCGGCCAATGGCCTCAGGCTATGGGCGCATGTGG GAAGACCCCATGGGGGCCCGGGGCCAGTGCATGTCTGGTGCCTCTCGGCTGCCCTCCCTCTTCTCCCAGA ACATCATCCCCGAGTACGGCATGTTCCAGGGCATGCGAGGTGGGGGCGCCTTCCCGGGCGGCTCCCGCTT TGGTTTCGGGTTTGGCAATGGCATGAAGCAGATGAGGCGGACCTGGAAGACCTGGACCACAGCCGACTTC CGAACCAAGAAGAAGAAGAGAAAGCAGGGCGGCAGTCCTGATGAGCCAGATAGCAAAGCCACCCGCACGG ACTGCTCGGACAACAGCGACTCAGACAATGATGAGGGCACCGAGGGGGAAGCCACAGAGGGCCTTGAAGG CACCGAGGCTGTGGAGAAGGGCTCCAGAGTGGACGGAGAGGATGAGGAGGGAAAAGAGGATGGGAGAGAA GAAGGCAAAGAGGATCCAGAGAAGGGGGCCCTAACCACCCAGGATGAAAATGGCCAGACCAAGCGCAAGT TGCAGGCAGGCAAGAAGAGTCAGGACAAGCAGAAAAAGCGGCAGCGAGACCGCATGGTGGAAAGGATCCA GTTTGTGTGTTCTCTGTGCAAATACCGGACCTTCTATGAGGACGAGATGGCCAGCCATCTTGACAGCAAG TTCCACAAGGAACACTTTAAGTACGTAGGCACCAAGCTCCCTAAGCAGACGGCTGACTTTCTGCAGGAGT ACGTCACTAACAAGACCAAGAAGACAGAGGAGCTCCGAAAAACCGTGGAGGACCTTGATGGCCTCATCCA GCAAATCTACAGAGACCAGGATCTGACCCAGGAAATTGCCATGGAGCATTTTGTGAAGAAGGTGGAGGCA GCCCATTGTGCAGCCTGCGACCTCTTCATTCCCATGCAGTTTGGGATCATCCAGAAGCATCTGAAGACCA TGGATCACAACCGGAACCGCAGGCTCATGATGGAGCAGTCCAAGAAGTCCTCCCTCATGGTGGCCCGCAG TATTCTCAACAACAAGCTCATCAGCAAGAAGCTGGAGCGCTACCTGAAGGGCGAGAACCCTTTCACCGAC AGCCCCGAGGAGGAGAAGGAGCAGGAGGAGGCTGAGGGCGGTGCCCTGGACGAGGGGGCGCAGGGCGAAG CGGCAGGGATCTCGGAGGGCGCAGAGGGCGTGCCGGCGCAGCCTCCCGTGCCCCCAGAGCCAGCCCCCGG GGCCGTGTCGCCGCCACCGCCGCCGCCCCCAGAGGAGGAGGAGGAGGGCGCCGTGCCCTTGCTGGGAGGG GCGCTGCAACGCCAGATCCGCGGCATCCCGGGCCTCGACGTGGAGGACGACGAGGAGGGCGGCGGGGGCG CCCCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC238988 representing NM_001291478 Red=Cloning site Green=Tags(s) MSYTGFVQGSETTLQSTYSDTSAQPTCDYGYGTWNSGTNRGYEGYGYGYGYGQDNTTNYGYDSYESCDSR AVLSERDLYRSGYDYSELDPEMEMAYEGQYDAYRDQFRMRGNDTFGPRAQGWARDARSGRPMASGYGRMW EDPMGARGQCMSGASRLPSLFSQNIIPEYGMFQGMRGGGAFPGGSRFGFGFGNGMKQMRRTWKTWTTADF RTKKKKRKQGGSPDEPDSKATRTDCSDNSDSDNDEGTEGEATEGLEGTEAVEKGSRVDGEDEEGKEDGRE EGKEDPEKGALTTQDENGQTKRKLQAGKKSQDKQKKRQRDRMVERIQFVCSLCKYRTFYEDEMASHLDSK FHKEHFKYVGTKLPKQTADFLQEYVTNKTKKTEELRKTVEDLDGLIQQIYRDQDLTQEIAMEHFVKKVEA AHCAACDLFIPMQFGIIQKHLKTMDHNRNRRLMMEQSKKSSLMVARSILNNKLISKKLERYLKGENPFTD SPEEEKEQEEAEGGALDEGAQGEAAGISEGAEGVPAQPPVPPEPAPGAVSPPPPPPPEEEEEGAVPLLGG ALQRQIRGIPGLDVEDDEEGGGGAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001291478
ORF Size	1755 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291478.2
RefSeq Size	2048 bp
RefSeq ORF	1758 bp
Locus ID	26993
UniProt ID	Q9ULX6
Protein Families	Druggable Genome
MW	65.7 kDa
Gene Summary	Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585). May be involved in regulation of pre-mRNA splicing (PubMed:17594903).[UniProtKB/Swiss-Prot Function]

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