AKAP8L (NM_001291478) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HA95; HAP95; NAKAP; NAKAP95
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336882 representing NM_001291478. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCTACACAGGCTTTGTCCAGGGATCTGAAACCACTTTGCAGTCGACATACTCGGATACCAGCGCT CAGCCCACCTGTGATTATGGATATGGAACTTGGAACTCTGGGACAAATAGAGGCTACGAGGGCTATGGC TATGGCTATGGCTATGGCCAGGATAACACCACCAACTATGGGTATGACTCTTATGAGTCCTGCGACTCG AGGGCCGTCCTGAGTGAGCGCGACCTGTACCGGTCAGGCTATGACTACAGCGAGCTTGACCCTGAGATG GAAATGGCCTATGAGGGCCAATACGATGCCTACCGCGACCAGTTCCGCATGCGTGGCAACGACACCTTC GGTCCCAGGGCACAGGGCTGGGCCCGGGATGCCCGGAGCGGCCGGCCAATGGCCTCAGGCTATGGGCGC ATGTGGGAAGACCCCATGGGGGCCCGGGGCCAGTGCATGTCTGGTGCCTCTCGGCTGCCCTCCCTCTTC TCCCAGAACATCATCCCCGAGTACGGCATGTTCCAGGGCATGCGAGGTGGGGGCGCCTTCCCGGGCGGC TCCCGCTTTGGTTTCGGGTTTGGCAATGGCATGAAGCAGATGAGGCGGACCTGGAAGACCTGGACCACA GCCGACTTCCGAACCAAGAAGAAGAAGAGAAAGCAGGGCGGCAGTCCTGATGAGCCAGATAGCAAAGCC ACCCGCACGGACTGCTCGGACAACAGCGACTCAGACAATGATGAGGGCACCGAGGGGGAAGCCACAGAG GGCCTTGAAGGCACCGAGGCTGTGGAGAAGGGCTCCAGAGTGGACGGAGAGGATGAGGAGGGAAAAGAG GATGGGAGAGAAGAAGGCAAAGAGGATCCAGAGAAGGGGGCCCTAACCACCCAGGATGAAAATGGCCAG ACCAAGCGCAAGTTGCAGGCAGGCAAGAAGAGTCAGGACAAGCAGAAAAAGCGGCAGCGAGACCGCATG GTGGAAAGGATCCAGTTTGTGTGTTCTCTGTGCAAATACCGGACCTTCTATGAGGACGAGATGGCCAGC CATCTTGACAGCAAGTTCCACAAGGAACACTTTAAGTACGTAGGCACCAAGCTCCCTAAGCAGACGGCT GACTTTCTGCAGGAGTACGTCACTAACAAGACCAAGAAGACAGAGGAGCTCCGAAAAACCGTGGAGGAC CTTGATGGCCTCATCCAGCAAATCTACAGAGACCAGGATCTGACCCAGGAAATTGCCATGGAGCATTTT GTGAAGAAGGTGGAGGCAGCCCATTGTGCAGCCTGCGACCTCTTCATTCCCATGCAGTTTGGGATCATC CAGAAGCATCTGAAGACCATGGATCACAACCGGAACCGCAGGCTCATGATGGAGCAGTCCAAGAAGTCC TCCCTCATGGTGGCCCGCAGTATTCTCAACAACAAGCTCATCAGCAAGAAGCTGGAGCGCTACCTGAAG GGCGAGAACCCTTTCACCGACAGCCCCGAGGAGGAGAAGGAGCAGGAGGAGGCTGAGGGCGGTGCCCTG GACGAGGGGGCGCAGGGCGAAGCGGCAGGGATCTCGGAGGGCGCAGAGGGCGTGCCGGCGCAGCCTCCC GTGCCCCCAGAGCCAGCCCCCGGGGCCGTGTCGCCGCCACCGCCGCCGCCCCCAGAGGAGGAGGAGGAG GGCGCCGTGCCCTTGCTGGGAGGGGCGCTGCAACGCCAGATCCGCGGCATCCCGGGCCTCGACGTGGAG GACGACGAGGAGGGCGGCGGGGGCGCCCCGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291478
Insert Size	1758 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291478.1
RefSeq Size	2048 bp
RefSeq ORF	1758 bp
Locus ID	26993
UniProt ID	Q9ULX6
Protein Families	Druggable Genome
MW	65.2 kDa
Gene Summary	Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585). May be involved in regulation of pre-mRNA splicing (PubMed:17594903).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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