DACT2 (NM_001286350) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bA503C24.7; C6orf116; DAPPER2; DPR2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239058 representing NM_001286350 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACTGGAGGCCCCGGTCGGTTGATGAGACTACTGTGCCAGCGTGGAGACCCCAGGCTACCGAGG AGGGCGCCAGGCCCCCAGGGAGCGTGGAGGATGCAGGCCAGCCGTGGGGCACATTCTGGCCCAGGCCTGT GTCTACAGGTGATCTTGACAGAGCCCTGCCGGCGGACACGGGGCTCCAGAAAGCCAGCGCGGACGCCGAG CTCCTCGGGCTCCTCTGCCAGGGGGTGGATATCCCGCTGCACGTGCCGGACCCCAAGTATCGGCAGGACC TGGTGTCCCAGGGCGGCAGGGAGGTGTACCCGTACCCCAGCCCCCTGCACGCCGTGGCTCTACAGAGCCC CCTGTTTGTCCTGACTAAGGAAACCCCACAGAGAGGTGGCCCCTCGTTCCCTAGGGAGAGCCCCAGGGGC CCCGCAGGTCTGAACACCATCCAGACTGGGCCGGTCCTCGAGGCTGGCCCGGCCAGGGCCAGAGCTTATA TCGACAGGTTGCTGCATCTGTGGGGCCGGGAGACCCCAGCAAAGGGTAGCGAGGGAGAACAGGGACCTCT AAGGCATGCAGCGTCCCCATCTCCACAGAGGCAGGGTGGCTGGAGTACAGACGGTGGAGGGCGACTGCTG GTCTTCGCCCCAGGGAGGGAGGACGAGGGAGGGCCAGCTCAGAGCAGGGGTGCCGGCAGGGGCGGGCCCC AGCAGCAGGGATACATGCCCCTTGAGGGTCCCCAGCAGTCTGGCAGCCTTCCAGAGGAGGGCTCCAAGCC CTCAAACAGCTGTGTCCTCAGGGAGACCATGGTGCAGGCTTCTCCCAGCTCAAAGGCCCAGCAGACACCC TCAGCTCAGGACTATGGACGAGGCAACATCATATCCCCATCCAGGATGCTGGACAAGAGCCCCTCACCGG CCTCTGGGCACTTTGCCCACCCATCCTTTGCTGCCAGCCTGAAAATGGGTCCCCCCAAGAGCAAGGCTGA AAAAATCAAGAGAAGTCCCATGGACAAGGTGCTGAGGTTTGCAAGGCAGCCGCTGCTTCTACTGGACAGG CCTGAGGGAGCCCATGCAGCCCCCCAGCCATCCCTGGAGTGGGACCCTGCCCACTGGCCCACAGGGAGGG GCGGGCTCCAGCGGAGGCCAGCCCTGGCCTGGGAGGCACCCGGGCGCTCCTGTTCTGAGTCCACCCTCTA CCCCATGCCTGTCCTCGTCCCCTTGGCAGTGGCCCCGCAGGAGAGCCACCGGACCTCAGCCCAAGCCCTG TTCCCCTTTGAGGCGTCACTGCTCACCTCAGTGGCCAGGAGGAAGCATCGCCGCTGGCAGTCCACCGTGG AGATCTCGGCCCGGGCCCGCCTGGCCAGCTGTCCTGAGTCTAACCTGGGGCCCCCCAGGCCCGTGGCCAG GAGAGCAGGTGGCCCACTGGCCCGGGGCCGTCCCTCACTGGTCCGCCAGGACGCCTACACCAGGAGCGAC TCAGAGCCCTCCAAGCACTCGGCCGAGTGTGACCCGCGGTTCCCGTCAGTCATCCCGGAGACCAGCGAGG GAGAGTCCAGTGACCACACCACCAACCGATTCGGAGACCGTGAGTCCAGCAGCAGCGACGAGGAGGGCGG CGCCCAGAGCAGGGACTGTGACCTGGCACTGGGCTATGTGGCGGCCGGGCATGCGGAGCTGGCCTGGACC CAGGAGGCCCCGGTCAGCTCGGGGCCACTCCTGTCCCCCGTGCCCAAGCTGTGCCGTATTAAGGCCTCCA AGGCCCTGAAGAAGAAGATCCGCAGGTTCCAGCCGACGGCCCTGAAGGTCATGACCATGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239058 representing NM_001286350 Red=Cloning site Green=Tags(s) MGDWRPRSVDETTVPAWRPQATEEGARPPGSVEDAGQPWGTFWPRPVSTGDLDRALPADTGLQKASADAE LLGLLCQGVDIPLHVPDPKYRQDLVSQGGREVYPYPSPLHAVALQSPLFVLTKETPQRGGPSFPRESPRG PAGLNTIQTGPVLEAGPARARAYIDRLLHLWGRETPAKGSEGEQGPLRHAASPSPQRQGGWSTDGGGRLL VFAPGREDEGGPAQSRGAGRGGPQQQGYMPLEGPQQSGSLPEEGSKPSNSCVLRETMVQASPSSKAQQTP SAQDYGRGNIISPSRMLDKSPSPASGHFAHPSFAASLKMGPPKSKAEKIKRSPMDKVLRFARQPLLLLDR PEGAHAAPQPSLEWDPAHWPTGRGGLQRRPALAWEAPGRSCSESTLYPMPVLVPLAVAPQESHRTSAQAL FPFEASLLTSVARRKHRRWQSTVEISARARLASCPESNLGPPRPVARRAGGPLARGRPSLVRQDAYTRSD SEPSKHSAECDPRFPSVIPETSEGESSDHTTNRFGDRESSSSDEEGGAQSRDCDLALGYVAAGHAELAWT QEAPVSSGPLLSPVPKLCRIKASKALKKKIRRFQPTALKVMTMV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286350
ORF Size	1812 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286350.2
RefSeq Size	2469 bp
RefSeq ORF	1815 bp
Locus ID	168002
UniProt ID	Q5SW24
MW	65.3 kDa
Gene Summary	Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling (By similarity).[UniProtKB/Swiss-Prot Function]

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