DACT2 (NM_001286350) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA503C24.7; C6orf116; DAPPER2; DPR2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336952 representing NM_001286350. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGGACTGGAGGCCCCGGTCGGTTGATGAGACTACTGTGCCAGCGTGGAGACCCCAGGCTACCGAG GAGGGCGCCAGGCCCCCAGGGAGCGTGGAGGATGCAGGCCAGCCGTGGGGCACATTCTGGCCCAGGCCT GTGTCTACAGGTGATCTTGACAGAGCCCTGCCGGCGGACACGGGGCTCCAGAAAGCCAGCGCGGACGCC GAGCTCCTCGGGCTCCTCTGCCAGGGGGTGGATATCCCGCTGCACGTGCCGGACCCCAAGTATCGGCAG GACCTGGTGTCCCAGGGCGGCAGGGAGGTGTACCCGTACCCCAGCCCCCTGCACGCCGTGGCTCTACAG AGCCCCCTGTTTGTCCTGACTAAGGAAACCCCACAGAGAGGTGGCCCCTCGTTCCCTAGGGAGAGCCCC AGGGGCCCCGCAGGTCTGAACACCATCCAGACTGGGCCGGTCCTCGAGGCTGGCCCGGCCAGGGCCAGA GCTTATATCGACAGGTTGCTGCATCTGTGGGGCCGGGAGACCCCAGCAAAGGGTAGCGAGGGAGAACAG GGACCTCTAAGGCATGCAGCGTCCCCATCTCCACAGAGGCAGGGTGGCTGGAGTACAGACGGTGGAGGG CGACTGCTGGTCTTCGCCCCAGGGAGGGAGGACGAGGGAGGGCCAGCTCAGAGCAGGGGTGCCGGCAGG GGCGGGCCCCAGCAGCAGGGATACATGCCCCTTGAGGGTCCCCAGCAGTCTGGCAGCCTTCCAGAGGAG GGCTCCAAGCCCTCAAACAGCTGTGTCCTCAGGGAGACCATGGTGCAGGCTTCTCCCAGCTCAAAGGCC CAGCAGACACCCTCAGCTCAGGACTATGGACGAGGCAACATCATATCCCCATCCAGGATGCTGGACAAG AGCCCCTCACCGGCCTCTGGGCACTTTGCCCACCCATCCTTTGCTGCCAGCCTGAAAATGGGTCCCCCC AAGAGCAAGGCTGAAAAAATCAAGAGAAGTCCCATGGACAAGGTGCTGAGGTTTGCAAGGCAGCCGCTG CTTCTACTGGACAGGCCTGAGGGAGCCCATGCAGCCCCCCAGCCATCCCTGGAGTGGGACCCTGCCCAC TGGCCCACAGGGAGGGGCGGGCTCCAGCGGAGGCCAGCCCTGGCCTGGGAGGCACCCGGGCGCTCCTGT TCTGAGTCCACCCTCTACCCCATGCCTGTCCTCGTCCCCTTGGCAGTGGCCCCGCAGGAGAGCCACCGG ACCTCAGCCCAAGCCCTGTTCCCCTTTGAGGCGTCACTGCTCACCTCAGTGGCCAGGAGGAAGCATCGC CGCTGGCAGTCCACCGTGGAGATCTCGGCCCGGGCCCGCCTGGCCAGCTGTCCTGAGTCTAACCTGGGG CCCCCCAGGCCCGTGGCCAGGAGAGCAGGTGGCCCACTGGCCCGGGGCCGTCCCTCACTGGTCCGCCAG GACGCCTACACCAGGAGCGACTCAGAGCCCTCCAAGCACTCGGCCGAGTGTGACCCGCGGTTCCCGTCA GTCATCCCGGAGACCAGCGAGGGAGAGTCCAGTGACCACACCACCAACCGATTCGGAGACCGTGAGTCC AGCAGCAGCGACGAGGAGGGCGGCGCCCAGAGCAGGGACTGTGACCTGGCACTGGGCTATGTGGCGGCC GGGCATGCGGAGCTGGCCTGGACCCAGGAGGCCCCGGTCAGCTCGGGGCCACTCCTGTCCCCCGTGCCC AAGCTGTGCCGTATTAAGGCCTCCAAGGCCCTGAAGAAGAAGATCCGCAGGTTCCAGCCGACGGCCCTG AAGGTCATGACCATGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286350
Insert Size	1815 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286350.1
RefSeq Size	2469 bp
RefSeq ORF	1815 bp
Locus ID	168002
UniProt ID	Q5SW24
MW	64.9 kDa
Gene Summary	Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks a portion of the 5' UTR and 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a.

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