ZNF539 (ZNF254) (NM_001278661) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BMZF-5; HD-ZNF1; ZNF91L; ZNF539
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239119 representing NM_001278661 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTAGAGAACTACAGAAACCTGGCCTTCCTGGGTATTGCTGTCTCTAAGCCAGACCTGATCACCTGTC TGGAACAAGGGAAAGAGCCCTGGAATATGAAGCGACATGAGATGGTGGATGAACCCCCAGGTATGTGTCC TCATTTTGCTCAAGACCTTTGGCCAGAGCAGGGCATGGAAGATTCTTTTCAAAAAGCAATACTGAGAAGA TATGGAAAATATGGACATGAGAATTTACAGTTAAGAAAAGGCTGTAAAAGTGTGGATGAGTATAAGGTGA ACAAAGAAGGTTATAATGGACTTAACCAGTGTTTCACAACTGCCCAGAGCAAAGTATTTCAATGTGATAA ATATTTGAAAGTCTTCTATAAATTTTTAAATTCAAACAGACCTAAGATAAGACATACTGAAAAGAAATCT TTCAAATGTAAAAAACGTGTCAAATTATTTTGCATGCTTTCACATAAAACCCAACACAAAAGCATTTATC ATAGAGAGAAGTCCTACAAATGTAAAGAATGTGGAAAAACCTTTAATTGGTCCTCAACCCTTACTAATCA TAGGAAAATTTATACTGAAGAGAAACCTTACAAATGTGAAGAATATAACAAATCTCCTAAGCAACTCTCA ACCCTTACTACACATGAAATAATTCATGCTGGAGAGAAACTCTACAAATGTGAAGAATGTGGTGAAGCTT TTAATCGATCCTCAAATCTTACTACACATAAGATAATTCATACTGGAGAGAAACCTTACAAGTGTGAAGA ATGTGGCAAAGCATTTATCTGGTCCTCAACCCTTACTGAGCATAAGAAAATTCATACTAGAAAGAAACCC TACAAGTGTGAAGAATGTGGCAAAGCATTTATATGGTCCTCAACACTAACTAGACATAAGAGGATGCACA CTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCTTTTAGCCAGTCCTCAACCCTTACTACACA TAAGATAATTCATACTGGAGAGAAACGCTACAAATGCTTAGAATGTGGCAAAGCTTTTAAGCAACTCTCA ACTCTTACTACACATAAAATAATTCATGTTGGAGAGAAACTCTACAAATGTGAAGAATGCGGCAAAGGTT TTAATCGATCTTCAAATCTTACTACACATAAGATAATTCATACTGGAGAGAAACCTTACAAGTGTGAAGA ATGTGGCAAAGCATTTATCTGGTCCTCAACCCTTACTAAACATAAGAGAATTCATACTAGAGAGAAACCC TACAAATGTGAAGAATGTGGCAAGGCATTTATATGGTCCTCAACCCTAACTAGACATAAGAGGATGCACA CTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAATCTTTTAGCCAATCCTCAACCCTTACTACACA TAAGATAATTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGCCTTTAACTGGTCCTCA ACTCTTACTAAACATAAGATAATTCATACTGAAGAGAAACCCTACAAATGTGAAAAATGTGGCAAAGCCT TTAAGCAGTCTTCAATCCTTACTAACCATAAGAGAATTCATACTGGAGAGAAACCCTATAAATGTGAAGA ATGTGGCAAATCTTTTAACCGGTCTTCAACTTTTACTAAACATAAGGTAATTCATACTGGAGTAAAACCC TACAAATGTGAAGAATGTGGCAAAGCATTTTTCTGGTCCTCAACCCTAACTAAACATAAGAGAATTCATA CTGGAGAGCAACCCTACAAATGGGAAAAATTTGGCAAAGCCTTTAATCGGTCCTCGCACCTCACCACAGA TAAGATAACTCATTGGAGAGAAATCTTACAAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239119 representing NM_001278661 Red=Cloning site Green=Tags(s) MLENYRNLAFLGIAVSKPDLITCLEQGKEPWNMKRHEMVDEPPGMCPHFAQDLWPEQGMEDSFQKAILRR YGKYGHENLQLRKGCKSVDEYKVNKEGYNGLNQCFTTAQSKVFQCDKYLKVFYKFLNSNRPKIRHTEKKS FKCKKRVKLFCMLSHKTQHKSIYHREKSYKCKECGKTFNWSSTLTNHRKIYTEEKPYKCEEYNKSPKQLS TLTTHEIIHAGEKLYKCEECGEAFNRSSNLTTHKIIHTGEKPYKCEECGKAFIWSSTLTEHKKIHTRKKP YKCEECGKAFIWSSTLTRHKRMHTGEKPYKCEECGKAFSQSSTLTTHKIIHTGEKRYKCLECGKAFKQLS TLTTHKIIHVGEKLYKCEECGKGFNRSSNLTTHKIIHTGEKPYKCEECGKAFIWSSTLTKHKRIHTREKP YKCEECGKAFIWSSTLTRHKRMHTGEKPYKCEECGKSFSQSSTLTTHKIIHTGEKPYKCEECGKAFNWSS TLTKHKIIHTEEKPYKCEKCGKAFKQSSILTNHKRIHTGEKPYKCEECGKSFNRSSTFTKHKVIHTGVKP YKCEECGKAFFWSSTLTKHKRIHTGEQPYKWEKFGKAFNRSSHLTTDKITHWREILQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001278661
ORF Size	1854 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278661.1, NP_001265590.1
RefSeq Size	4262 bp
RefSeq ORF	1857 bp
Locus ID	9534
Protein Families	Transcription Factors
MW	72.8 kDa
Gene Summary	Zinc finger proteins have been shown to interact with nucleic acids and to have diverse functions. The zinc finger domain is a conserved amino acid sequence motif containing 2 specifically positioned cysteines and 2 histidines that are involved in coordinating zinc. Kruppel-related proteins form 1 family of zinc finger proteins. See ZFP93 (MIM 604749) for additional information on zinc finger proteins.[supplied by OMIM, Jul 2002]

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