ASCC3 (NM_001284271) Human Tagged ORF Clone
CAT#: RC239435
- TrueORF®
ASCC3 (myc-DDK-tagged) - Human activating signal cointegrator 1 complex subunit 3 (ASCC3), transcript variant 3
ORF Plasmid: tGFP
"NM_001284271" in other vectors (2)
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CNY 5,888.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ASC1p200; HELIC1; RNAH |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239435 representing NM_001284271
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTACCTCGTCTCACAGGAGCCTTGCGTTCCTTTTCAAATGTCACCAAGCAAGATAATTATAATG AAGAAGTGGCTGACTTAAAGATAAAGCGATCTAAACTTCATGAACAAGTTTTAGATTTGGGCCTGACATG GAAGAAGATAATAAAATTTTTGAATGAAAAACTGGAGAAGAGTAAAATGCAAAGTATAAATGAAGACTTA AAAGATATATTACATGCTGCAAAGCAGATAGTTGGAACTGATAATGGGAGAGAAGCAATTGAAAGTGGGG CTGCATTTCTCTTCATGACATTTCACTTGAAGGACTCTGTTGGTCACAAGGAAACAAAGGCTATCAAACA GATGTTTGGCCCCTTTCCTTCATCATCTGCCACTGCAGCTTGTAATGCTACTAATCGAATTATTTCTCAT TTTAGTCAAGATGATCTTACTGCTCTTGTGCAGATGACAGAAAAAGAACATGGCGATAGGGTTTTTTTTG GTAAAAATTTAGCATTTTCATTTGACATGCATGATTTGGACCACTTTGACGAACTGCCAATAAATGGTGA AACTCAGAAAACTATAAGCCTAGATTATAAGAAGTTTCTGAATGAACATCTCCAGGAGGCTTGCACCCCA GAACTCAAGCCTGTGGAAAAAACAAATGGCTCCTTTTTGTGGTGTGAAGTTGAAAAGTACCTAAATTCAA CTTTGAAGGAAATGACTGAAGTGCCAAGAGTAGAAGATCTTTGCTGTACTTTATATGATATGCTTGCTTC TATTAAAAGTGGTGATGAACTTCAGGATGAGCTATTTGAACTGCTGGGACCTGAAGGACTTGAACTTATT GAGAAACTCCTCCAGAACAGAATTACAATTGTGGATAGATTTCTTAATTCTTCAAATGATCATAGGTTTC AGGCTCTTCAAGACAATTGTAAAAAAATTTTAGGAGAAAATGCTAAACCCAATTATGGTTGTCAAGTCAC TATTCAGTCTGAACAAGAAAAGCAGTTAATGAAACAATATCGACGTGAAGAAAAAAGAATTGCCAGACGA GAAAAAAAGGCTGGAGAAGATTTAGAAGTTTCAGAAGGACTTATGTGCTTTGATCCTAAGGAATTGCGGA TACAAAGAGAACAGGCACTTCTGAATGCTAGAAGTGTTCCAATTCTGAGCAGGCAGAGAGATGCAGACGT TGAAAAAATACATTATCCCCATGTGTATGATTCCCAGGCTGAAGCCATGAAAACATCAGCATTTATTGCT GGTGCAAAGATGATTTTGCCAGAAGGAATCCAAAGAGAGAATAACAAGCTTTATGAAGAAGTAAGGATTC CCTACAGCGAACCAATGCCACTCAGCTTTGAGGAAAAGCCAGTTTATATCCAAGACTTAGATGAGATCGG ACAGCTGGCTTTTAAAGGAATGAAGAGACTCAATAGAATCCAGTCAATAGTGTTTGAGACTGCCTACAAC ACCAATGAGAACATGCTGATTTGTGCCCCTACAGGAGCTGGAAAAACCAACATTGCAATGCTGACAGTCT TGCATGAAATTCGCCAACATTTTCAACAAGGTGTTATCAAAAAGAATGAATTTAAGATTGTATATGTTGC TCCAATGAAAGCCTTGGCAGCTGAAATGACAGATTACTTCAGCAGACGTCTAGAGCCACTAGGCATCATT GTGAAAGAATTGACTGGTGACATGCAGTTGTCCAAAAGTGAAATTTTACGAACTCAGATGCTTGTGACCA CACCAGAAAAATGGGATGTAGTGACAAGAAAGAGTGTTGGGGATGTAGCTCTTTCCCAGATTGTAAGGCT CCTTATTCTTGATGAAGTTCATTTGCTGCATGAAGATAGAGGACCAGTATTAGAAAGCATAGTTGCCCGT ACTTTACGGCAGGTGGAATCCACACAGAGTATGATAAGGATTCTCGGACTGTCTGCAACTTTACCTAACT ACCTCGATGTTGCCACATTTTTACATGTTAATCCATACATTGGACTTTTCTTCTTTGATGGCCGTTTTCG ACCAGTACCTCTTGGACAGACATTTTTGGGGATTAAATGTGCAAATAAGATGCAGCAGTTGAATAACATG GATGAAGTATGTTATGAAAATGTTTTGAAGCAAGTAAAGGCTGGACACCAGGTACACTTATTTTATCTGC TTTTACACTTATTTATCTGCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239435 representing NM_001284271
Red=Cloning site Green=Tags(s) MALPRLTGALRSFSNVTKQDNYNEEVADLKIKRSKLHEQVLDLGLTWKKIIKFLNEKLEKSKMQSINEDL KDILHAAKQIVGTDNGREAIESGAAFLFMTFHLKDSVGHKETKAIKQMFGPFPSSSATAACNATNRIISH FSQDDLTALVQMTEKEHGDRVFFGKNLAFSFDMHDLDHFDELPINGETQKTISLDYKKFLNEHLQEACTP ELKPVEKTNGSFLWCEVEKYLNSTLKEMTEVPRVEDLCCTLYDMLASIKSGDELQDELFELLGPEGLELI EKLLQNRITIVDRFLNSSNDHRFQALQDNCKKILGENAKPNYGCQVTIQSEQEKQLMKQYRREEKRIARR EKKAGEDLEVSEGLMCFDPKELRIQREQALLNARSVPILSRQRDADVEKIHYPHVYDSQAEAMKTSAFIA GAKMILPEGIQRENNKLYEEVRIPYSEPMPLSFEEKPVYIQDLDEIGQLAFKGMKRLNRIQSIVFETAYN TNENMLICAPTGAGKTNIAMLTVLHEIRQHFQQGVIKKNEFKIVYVAPMKALAAEMTDYFSRRLEPLGII VKELTGDMQLSKSEILRTQMLVTTPEKWDVVTRKSVGDVALSQIVRLLILDEVHLLHEDRGPVLESIVAR TLRQVESTQSMIRILGLSATLPNYLDVATFLHVNPYIGLFFFDGRFRPVPLGQTFLGIKCANKMQQLNNM DEVCYENVLKQVKAGHQVHLFYLLLHLFICF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001284271 |
ORF Size | 2193 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001284271.1, NP_001271200.1 |
RefSeq Size | 2849 bp |
RefSeq ORF | 2196 bp |
Locus ID | 10973 |
UniProt ID | Q8N3C0 |
Protein Families | Transcription Factors |
MW | 84.1 kDa |
Gene Summary | This gene encodes a protein that belongs to a family of helicases that are involved in the ATP-dependent unwinding of nucleic acid duplexes. The encoded protein is the largest subunit of the activating signal cointegrator 1 complex that is involved in DNA repair and resistance to alkylation damage. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
Documents
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FAQs |
SDS |