HA1 (ARHGAP45) (NM_001282334) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HA-1; HLA-HA1; HMHA1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239534 representing NM_001282334 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGTGGGGAGGAAAGGAGGAGCTGGGGAGACTGAGTCCCATCCGAGGATAGGGTTGGAACTGGCCT CCTGGCTCCCACACCCACAGCAAGAGGCGGAGTCCAACCTGCGCAAGGCCAAGCAGGGTTACGTGCAGCG CTGCGAGGACCACGACAAGGCTCGCTTCCTCGTGGCCAAGGCGGAGGAGGAGCAGGCTGGCAGCGCGCCG GGAGCAGGCAGCACGGCCACCAAGACCCTGGACAAGCGGCGGCGGCTGGAGGAGGAGGCCAAGAACAAGG CGGAGGAAGCTATGGCCACCTACCGCACCTGCGTGGCCGACGCGAAGACGCAGAAGCAGGAGCTGGAGGA TACCAAGGTGACGGCGCTGCGGCAGATCCAGGAGGTCATCCGGCAGAGCGACCAAACCATCAAGTCGGCC ACGATCTCCTACTACCAGATGATGCATATGCAGACGGCGCCGCTGCCCGTGCACTTCCAGATGCTGTGTG AGAGCAGCAAGCTGTATGACCCAGGCCAGCAGTACGCCTCCCACGTGCGCCAGCTGCAGCGGGACCAGGA GCCCGATGTGCACTACGACTTTGAGCCCCACGTCTCCGCCAACGCCTGGTCCCCCGTCATGCGTGCCCGG AAGAGCAGCTTCAACGTGAGTGATGTGGCGCGGCCGGAGGCTGCCGGGAGCCCCCCAGAAGAAGGCGGGT GCACTGAGGGCACACCTGCCAAGGACCACAGGGCCGGGCGAGGACACCAGGTTCACAAGTCATGGCCGCT CTCGATCTCAGACTCGGACAGTGGGCTGGACCCCGGCCCTGGCGCAGGGGACTTTAAGAAGTTCGAGCGG ACGTCATCCAGTGGTACCATGTCGTCCACGGAGGAGCTGGTGGACCCAGACGGTGGAGCCGGGGCTTCAG CCTTTGAGCAGGCTGACCTCAACGGCATGACCCCCGAGCTGCCGGTGGCCGTGCCCAGTGGACCGTTCCG CCACGAGGGGCTGTCCAAGGCGGCCCGTACTCACCGGCTCCGGAAGCTCCGCACGCCCGCCAAGTGCCGC GAGTGCAACAGCTACGTCTACTTCCAGGGTGCTGAGTGTGAAGAGTGCTGCCTGGCCTGCCACAAGAAAT GTCTGGAGACGCTGGCCATACAGTGCGGGCACAAGAAGCTGCAAGGCCGCCTGCAGCTGTTCGGCCAGGA CTTCAGCCACGCGGCCCGCAGCGCCCCCGACGGCGTGCCCTTCATCGTCAAGAAGTGCGTCTGCGAGATC GAGCGGCGGGCGCTGCGCACCAAGGGCATCTACCGGGTCAATGGGGTAAAGACACGCGTGGAGAAGCTGT GCCAGGCCTTCGAGAACGGCAAGGAGCTGGTCGAGCTGTCGCAGGCCTCGCCCCACGACATCAGCAACGT CCTCAAGCTCTACCTGCGTCAGCTTCCCGAGCCGCTCATCTCCTTCCGCCTCTACCACGAGCTCGTAGGG CTGGCCAAGGACAGCCTGAAGGCAGAGGCCGAGGCCAAGGCGGCGTCCCGGGGCCGGCAGGACGGCTCGG AGAGCGAGGCAGTGGCGGTGGCCCTGGCAGGTCGGCTGCGGGAGCTCCTGCGGGACCTGCCGCCTGAGAA CCGGGCCTCGCTGCAGTACCTGCTGCGTCACCTACGCAGGATCGTGGAGGTGGAGCAGGACAACAAGATG ACCCCCGGGAACCTGGGCATCGTGTTCGGGCCCACGCTGCTTCGGCCACGGCCCACCGAGGCCACCGTGT CCCTCTCCTCCCTGGTGGATTATCCCCATCAGGCCCGCGTCATCGAGACTCTCATCGTCCACTACGGCCT GGTCTTCGAGGAGGAGCCGGAGGAGACCCCCGGGGGCCAGGACGAGTCATCCAACCAGCGAGCTGAGGTA GTCGTCCAGGTGCCGTACCTGGAGGCGGGCGAGGCGGTGGTCTACCCGCTGCAGGAGGCGGCGGCGGACG GGTGCAGAGAATCCCGAGTTGTGTCCAACGATTCGGACTCGGACCTAGAGGAGGCCTCCGAGCTGCTGTC CTCATCGGAGGCCAGTGCCCTGGGCCACCTCAGCTTCCTGGAGCAGCAGCAGAGCGAGGCCAGCCTAGAG GTGGCTTCTGGCAGCCACAGCGGCAGTGAGGAGCAGCTGGAGGCCACAGCCCGGGAGGACGGGGACGGGG ACGAGGACGGCCCGGCCCAGCAGCTCTCAGGATTCAACACCAACCAGTCCAACAACGTGCTGCAGGCCCC ACTGCCCCCCATGAGGCTCCGTGGCGGGCGGATGACACTGGGCTCCTGCAGGGAAAGGCAGCCGGAATTC GTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239534 representing NM_001282334 Red=Cloning site Green=Tags(s) MGVGRKGGAGETESHPRIGLELASWLPHPQQEAESNLRKAKQGYVQRCEDHDKARFLVAKAEEEQAGSAP GAGSTATKTLDKRRRLEEEAKNKAEEAMATYRTCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSA TISYYQMMHMQTAPLPVHFQMLCESSKLYDPGQQYASHVRQLQRDQEPDVHYDFEPHVSANAWSPVMRAR KSSFNVSDVARPEAAGSPPEEGGCTEGTPAKDHRAGRGHQVHKSWPLSISDSDSGLDPGPGAGDFKKFER TSSSGTMSSTEELVDPDGGAGASAFEQADLNGMTPELPVAVPSGPFRHEGLSKAARTHRLRKLRTPAKCR ECNSYVYFQGAECEECCLACHKKCLETLAIQCGHKKLQGRLQLFGQDFSHAARSAPDGVPFIVKKCVCEI ERRALRTKGIYRVNGVKTRVEKLCQAFENGKELVELSQASPHDISNVLKLYLRQLPEPLISFRLYHELVG LAKDSLKAEAEAKAASRGRQDGSESEAVAVALAGRLRELLRDLPPENRASLQYLLRHLRRIVEVEQDNKM TPGNLGIVFGPTLLRPRPTEATVSLSSLVDYPHQARVIETLIVHYGLVFEEEPEETPGGQDESSNQRAEV VVQVPYLEAGEAVVYPLQEAAADGCRESRVVSNDSDSDLEEASELLSSSEASALGHLSFLEQQQSEASLE VASGSHSGSEEQLEATAREDGDGDEDGPAQQLSGFNTNQSNNVLQAPLPPMRLRGGRMTLGSCRERQPEF V SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282334
ORF Size	2313 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282334.1, NP_001269263.1
RefSeq Size	5286 bp
RefSeq ORF	2316 bp
Locus ID	23526
UniProt ID	Q92619
MW	85.1 kDa
Gene Summary	Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.[UniProtKB/Swiss-Prot Function]

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