HA1 (ARHGAP45) (NM_001282334) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HA-1; HLA-HA1; HMHA1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282334, the custom clone sequence may differ by one or more nucleotides ATGGGGGTGGGGAGGAAAGGAGGAGCTGGGGAGACTGAGTCCCATCCGAGGATAGGGTTGGAACTGGCCT CCTGGCTCCCACACCCACAGCAAGAGGCGGAGTCCAACCTGCGCAAGGCCAAGCAGGGTTACGTGCAGCG CTGCGAGGACCACGACAAGGCTCGCTTCCTCGTGGCCAAGGCGGAGGAGGAGCAGGCTGGCAGCGCGCCG GGAGCAGGCAGCACGGCCACCAAGACCCTGGACAAGCGGCGGCGGCTGGAGGAGGAGGCCAAGAACAAGG CGGAGGAAGCTATGGCCACCTACCGCACCTGCGTGGCCGACGCGAAGACGCAGAAGCAGGAGCTGGAGGA TACCAAGGTGACGGCGCTGCGGCAGATCCAGGAGGTCATCCGGCAGAGCGACCAAACCATCAAGTCGGCC ACGATCTCCTACTACCAGATGATGCATATGCAGACGGCGCCGCTGCCCGTGCACTTCCAGATGCTGTGTG AGAGCAGCAAGCTGTATGACCCAGGCCAGCAGTACGCCTCCCACGTGCGCCAGCTGCAGCGGGACCAGGA GCCCGATGTGCACTACGACTTTGAGCCCCACGTCTCCGCCAACGCCTGGTCCCCCGTCATGCGTGCCCGG AAGAGCAGCTTCAACGTGAGTGATGTGGCGCGGCCGGAGGCTGCCGGGAGCCCCCCAGAAGAAGGCGGGT GCACTGAGGGCACACCTGCCAAGGACCACAGGGCCGGGCGAGGACACCAGGTTCACAAGTCATGGCCGCT CTCGATCTCAGACTCGGACAGTGGGCTGGACCCCGGCCCTGGCGCAGGGGACTTTAAGAAGTTCGAGCGG ACGTCATCCAGTGGTACCATGTCGTCCACGGAGGAGCTGGTGGACCCAGACGGTGGAGCCGGGGCTTCAG CCTTTGAGCAGGCTGACCTCAACGGCATGACCCCCGAGCTGCCGGTGGCCGTGCCCAGTGGACCGTTCCG CCACGAGGGGCTGTCCAAGGCGGCCCGTACTCACCGGCTCCGGAAGCTCCGCACGCCCGCCAAGTGCCGC GAGTGCAACAGCTACGTCTACTTCCAGGGTGCTGAGTGTGAAGAGTGCTGCCTGGCCTGCCACAAGAAAT GTCTGGAGACGCTGGCCATACAGTGCGGGCACAAGAAGCTGCAAGGCCGCCTGCAGCTGTTCGGCCAGGA CTTCAGCCACGCGGCCCGCAGCGCCCCCGACGGCGTGCCCTTCATCGTCAAGAAGTGCGTCTGCGAGATC GAGCGGCGGGCGCTGCGCACCAAGGGCATCTACCGGGTCAATGGGGTAAAGACACGCGTGGAGAAGCTGT GCCAGGCCTTCGAGAACGGCAAGGAGCTGGTCGAGCTGTCGCAGGCCTCGCCCCACGACATCAGCAACGT CCTCAAGCTCTACCTGCGTCAGCTTCCCGAGCCGCTCATCTCCTTCCGCCTCTACCACGAGCTCGTAGGG CTGGCCAAGGACAGCCTGAAGGCAGAGGCCGAGGCCAAGGCGGCGTCCCGGGGCCGGCAGGACGGCTCGG AGAGCGAGGCAGTGGCGGTGGCCCTGGCAGGTCGGCTGCGGGAGCTCCTGCGGGACCTGCCGCCTGAGAA CCGGGCCTCGCTGCAGTACCTGCTGCGTCACCTACGCAGGATCGTGGAGGTGGAGCAGGACAACAAGATG ACCCCCGGGAACCTGGGCATCGTGTTCGGGCCCACGCTGCTTCGGCCACGGCCCACCGAGGCCACCGTGT CCCTCTCCTCCCTGGTGGATTATCCCCATCAGGCCCGCGTCATCGAGACTCTCATCGTCCACTACGGCCT GGTCTTCGAGGAGGAGCCGGAGGAGACCCCCGGGGGCCAGGACGAGTCATCCAACCAGCGAGCTGAGGTA GTCGTCCAGGTGCCGTACCTGGAGGCGGGCGAGGCGGTGGTCTACCCGCTGCAGGAGGCGGCGGCGGACG GGTGCAGAGAATCCCGAGTTGTGTCCAACGATTCGGACTCGGACCTAGAGGAGGCCTCCGAGCTGCTGTC CTCATCGGAGGCCAGTGCCCTGGGCCACCTCAGCTTCCTGGAGCAGCAGCAGAGCGAGGCCAGCCTAGAG GTGGCTTCTGGCAGCCACAGCGGCAGTGAGGAGCAGCTGGAGGCCACAGCCCGGGAGGACGGGGACGGGG ACGAGGACGGCCCGGCCCAGCAGCTCTCAGGATTCAACACCAACCAGTCCAACAACGTGCTGCAGGCCCC ACTGCCCCCCATGAGGCTCCGTGGCGGGCGGATGACACTGGGCTCCTGCAGGGAAAGGCAGCCGGAATTC GTGTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001282334
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282334.1, NP_001269263.1
RefSeq Size	5286 bp
RefSeq ORF	2316 bp
Locus ID	23526
UniProt ID	Q92619
Gene Summary	Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate internal promoter and thus differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at an alternate AUG, and the resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.