BTBD7 (NM_001289133) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	FUP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239550 representing NM_001289133 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGGGGTGCTGGCGCCGGTGCGGACGGCGGTGCTGGTGGCGGCGGCGGCGGAGGCGACGGCAGCG GTCCCAGCGGCAGCAGCAGCGGCGGGAGGAGCCTCCGGGGCTGTGAGGATATCATTGCTGAGAGCATCTC ATTAGATACCTTAATTGCCATCCTCAAGTGGAGTTCTCATCCATATGGCTCTAAATGGGTGCACCGACAA GCTTTACATTTCCTCTGTGAGGAATTTTCCCAGGTCATGACTTCGGATGTTTTTTATGAACTCAGCAAAG ACCATCTGCTTACTGCTATCCAGTCTGACTACCTACAGGCAAGTGAACAAGATATCCTTAAATATCTGAT TAAATGGGGAGAGCATCAGTTGATGAAAAGAATAGCAGATAGAGAGCCAAACTTACTGAGTGGCACTGCC CATAGTGTGAACAAAAGAGGTGTAAAAAGACGGGACCTGGACATGGAAGAGCTCAGAGAGATCCTTTCTT CTCTCTTACCTTTTGTGCGAATTGAACACATCTTACCTATAAACAGTGAAGTCTTAAGTGATGCAATGAA AAGAGGCTTGATTAGTACTCCTCCATCAGATATGCTTCCTACAACAGAAGGTGGGAAGTCAAATGCCTGG TTACGGCAAAAAAATGCTGGCATCTATGTTCGTCCTCGACTCTTCTCTCCCTATGTGGAAGAAGCAAAGT CAGTGCTAGATGAGATGATGGTGGAACAAACGGATCTTGTGCGCTTGCGAATGGTTAGAATGTCCAATGT GCCAGACACGCTCTACATGGTCAATAATGCCGTGCCACAGTGTTGTCACATGATCAGCCACCAGCAGATC AGCAGCAACCAGTCAAGCCCTCCTTCAGTTGTAGCCAACGAAATTCCAGTTCCTCGTCTCCTCATTATGA AAGACATGGTCAGACGACTGCAGGAACTGCGGCACACGGAGCAGGTGCAGAGGGCCTATGCCCTGAACTG CGGGGAAGGCGCCACTGTCAGCTATGAAATTCAGATTCGAGTGCTAAGAGAGTTTGGTCTTGCAGATGCT GCTGCAGAGCTGTTGCAGAATCCTCACAAATTCTTTCCTGATGAACGTTTTGGGGATGAAAGTCCACTCT TGACAATGAGACAGCCTGGGAGATGTCGCGTAAACAGTACACCTCCTGCAGAAACCATGTTTACAGATCT GGACTCTTTTGTGGCCTTCCATCCACCCTTGCCCCCTCCACCACCTCCCTACCACCCCCCAGCTACCCCA ATCCATAACCAACTCAAAGCAGGCTGGAAGCAAAGACCTCCCAGTCAGCACCCTTCACGTTCATTTTCTT ATCCCTGTAATCATTCGCTGTTCCACTCCAGAACAGCTCCTAAAGCTGGCCCTCCCCCAGTCTACTTGCC GAGTGTGAAAGCTGCACCGCCTGATTGTACCAGCACTGCAGGACTGGGCAGACAGACGGTGGCTGCTGCT GCCGCCACCACCACCTCAACAGCAACAGCAGCAGCAGCAGCAGCATCTGAGAAGCAAGTGCGAACACAAC CTGTGCTGAATGATCTGATGCCAGACATCGCGGTGGGTGTGTCCACACTGTCACTCAAGGACAGGAGGCT TCCAGAGCTTGCTGTAGACACAGAATTAAGCCAGTCAGTTTCTGAAGCAGGACCAGGGCCTCCCCAGCAT CTGTCGTGTATTCCACAGAGACATACACACACTTCTCGGAAAAAACACACACTAGAGCAAAAAACAGACA CCAGAGAAAATCCACAGGAATATCCGGATTTCTATGACTTCTCAAATGCTGCTTGCAGACCTTCTACTCC TGCTCTCAGCAGACGCACCCCTTCCCCTTCGCAAGGTGGATATTTTGGTCCCGATCTGTACAGCCACAAT AAGGCATCACCAAGTGGCTTAAAGTCAGCCTACCTACCTGGTCAGACGTCTCCTAAAAAACAGGAAGAAG CTAGGAGAGAATATCCACTTTCCCCTGACGGGCATCTACACAGACAAAAGAATGAGCCGATACACCTGGA TGTCGTTGAGCAACCTCCCCAGCGGTCAGACTTTCCTTTGGCAGCCCCAGAAAATGCTAGTACCGGTCCA GCCCATGTCAGGGGACGAACTGCAGTAGAAACTGACTTGACTTTTGGGCTGACTCCTAACAGACCTTCAC TTTCTGCATGTAGCTCTGAAGCTCCCGAAGAGAGATCCGGTAGAAGACTGGCAGACAGTGAGTCCCTGGG CCATGGAGCTCAGAGAAATACAGATTTGGAAAGGGAAGATTCAATAAGCAGAGGAAGGAGGTCACCAAGC AAGCCGGACTTCCTCTACAAAAAGTCTGCCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239550 representing NM_001289133 Red=Cloning site Green=Tags(s) MAGGAGAGADGGAGGGGGGGDGSGPSGSSSGGRSLRGCEDIIAESISLDTLIAILKWSSHPYGSKWVHRQ ALHFLCEEFSQVMTSDVFYELSKDHLLTAIQSDYLQASEQDILKYLIKWGEHQLMKRIADREPNLLSGTA HSVNKRGVKRRDLDMEELREILSSLLPFVRIEHILPINSEVLSDAMKRGLISTPPSDMLPTTEGGKSNAW LRQKNAGIYVRPRLFSPYVEEAKSVLDEMMVEQTDLVRLRMVRMSNVPDTLYMVNNAVPQCCHMISHQQI SSNQSSPPSVVANEIPVPRLLIMKDMVRRLQELRHTEQVQRAYALNCGEGATVSYEIQIRVLREFGLADA AAELLQNPHKFFPDERFGDESPLLTMRQPGRCRVNSTPPAETMFTDLDSFVAFHPPLPPPPPPYHPPATP IHNQLKAGWKQRPPSQHPSRSFSYPCNHSLFHSRTAPKAGPPPVYLPSVKAAPPDCTSTAGLGRQTVAAA AATTTSTATAAAAAASEKQVRTQPVLNDLMPDIAVGVSTLSLKDRRLPELAVDTELSQSVSEAGPGPPQH LSCIPQRHTHTSRKKHTLEQKTDTRENPQEYPDFYDFSNAACRPSTPALSRRTPSPSQGGYFGPDLYSHN KASPSGLKSAYLPGQTSPKKQEEARREYPLSPDGHLHRQKNEPIHLDVVEQPPQRSDFPLAAPENASTGP AHVRGRTAVETDLTFGLTPNRPSLSACSSEAPEERSGRRLADSESLGHGAQRNTDLEREDSISRGRRSPS KPDFLYKKSAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289133
ORF Size	2343 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289133.2
RefSeq Size	7233 bp
RefSeq ORF	2346 bp
Locus ID	55727
UniProt ID	Q9P203
MW	86 kDa
Gene Summary	Acts as a mediator of epithelial dynamics and organ branching by promoting cleft progression. Induced following accumulation of fibronectin in forming clefts, leading to local expression of the cell-scattering SNAIL2 and suppression of E-cadherin levels, thereby altering cell morphology and reducing cell-cell adhesion. This stimulates cell separation at the base of forming clefts by local, dynamic intercellular gap formation and promotes cleft progression (By similarity).[UniProtKB/Swiss-Prot Function]

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