BTBD7 (NM_001289133) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FUP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001289133, the custom clone sequence may differ by one or more nucleotides ATGGCCGGGGGTGCTGGCGCCGGTGCGGACGGCGGTGCTGGTGGCGGCGGCGGCGGAGGCGACGGCAGCG GTCCCAGCGGCAGCAGCAGCGGCGGGAGGAGCCTCCGGGGCTGTGAGGATATCATTGCTGAGAGCATCTC ATTAGATACCTTAATTGCCATCCTCAAGTGGAGTTCTCATCCATATGGCTCTAAATGGGTGCACCGACAA GCTTTACATTTCCTCTGTGAGGAATTTTCCCAGGTCATGACTTCGGATGTTTTTTATGAACTCAGCAAAG ACCATCTGCTTACTGCTATCCAGTCTGACTACCTACAGGCAAGTGAACAAGATATCCTTAAATATCTGAT TAAATGGGGAGAGCATCAGTTGATGAAAAGAATAGCAGATAGAGAGCCAAACTTACTGAGTGGCACTGCC CATAGTGTGAACAAAAGAGGTGTAAAAAGACGGGACCTGGACATGGAAGAGCTCAGAGAGATCCTTTCTT CTCTCTTACCTTTTGTGCGAATTGAACACATCTTACCTATAAACAGTGAAGTCTTAAGTGATGCAATGAA AAGAGGCTTGATTAGTACTCCTCCATCAGATATGCTTCCTACAACAGAAGGTGGGAAGTCAAATGCCTGG TTACGGCAAAAAAATGCTGGCATCTATGTTCGTCCTCGACTCTTCTCTCCCTATGTGGAAGAAGCAAAGT CAGTGCTAGATGAGATGATGGTGGAACAAACGGATCTTGTGCGCTTGCGAATGGTTAGAATGTCCAATGT GCCAGACACGCTCTACATGGTCAATAATGCCGTGCCACAGTGTTGTCACATGATCAGCCACCAGCAGATC AGCAGCAACCAGTCAAGCCCTCCTTCAGTTGTAGCCAACGAAATTCCAGTTCCTCGTCTCCTCATTATGA AAGACATGGTCAGACGACTGCAGGAACTGCGGCACACGGAGCAGGTGCAGAGGGCCTATGCCCTGAACTG CGGGGAAGGCGCCACTGTCAGCTATGAAATTCAGATTCGAGTGCTAAGAGAGTTTGGTCTTGCAGATGCT GCTGCAGAGCTGTTGCAGAATCCTCACAAATTCTTTCCTGATGAACGTTTTGGGGATGAAAGTCCACTCT TGACAATGAGACAGCCTGGGAGATGTCGCGTAAACAGTACACCTCCTGCAGAAACCATGTTTACAGATCT GGACTCTTTTGTGGCCTTCCATCCACCCTTGCCCCCTCCACCACCTCCCTACCACCCCCCAGCTACCCCA ATCCATAACCAACTCAAAGCAGGCTGGAAGCAAAGACCTCCCAGTCAGCACCCTTCACGTTCATTTTCTT ATCCCTGTAATCATTCGCTGTTCCACTCCAGAACAGCTCCTAAAGCTGGCCCTCCCCCAGTCTACTTGCC GAGTGTGAAAGCTGCACCGCCTGATTGTACCAGCACTGCAGGACTGGGCAGACAGACGGTGGCTGCTGCT GCCGCCACCACCACCTCAACAGCAACAGCAGCAGCAGCAGCAGCATCTGAGAAGCAAGTGCGAACACAAC CTGTGCTGAATGATCTGATGCCAGACATCGCGGTGGGTGTGTCCACACTGTCACTCAAGGACAGGAGGCT TCCAGAGCTTGCTGTAGACACAGAATTAAGCCAGTCAGTTTCTGAAGCAGGACCAGGGCCTCCCCAGCAT CTGTCGTGTATTCCACAGAGACATACACACACTTCTCGGAAAAAACACACACTAGAGCAAAAAACAGACA CCAGAGAAAATCCACAGGAATATCCGGATTTCTATGACTTCTCAAATGCTGCTTGCAGACCTTCTACTCC TGCTCTCAGCAGACGCACCCCTTCCCCTTCGCAAGGTGGATATTTTGGTCCCGATCTGTACAGCCACAAT AAGGCATCACCAAGTGGCTTAAAGTCAGCCTACCTACCTGGTCAGACGTCTCCTAAAAAACAGGAAGAAG CTAGGAGAGAATATCCACTTTCCCCTGACGGGCATCTACACAGACAAAAGAATGAGCCGATACACCTGGA TGTCGTTGAGCAACCTCCCCAGCGGTCAGACTTTCCTTTGGCAGCCCCAGAAAATGCTAGTACCGGTCCA GCCCATGTCAGGGGACGAACTGCAGTAGAAACTGACTTGACTTTTGGGCTGACTCCTAACAGACCTTCAC TTTCTGCATGTAGCTCTGAAGCTCCCGAAGAGAGATCCGGTAGAAGACTGGCAGACAGTGAGTCCCTGGG CCATGGAGCTCAGAGAAATACAGATTTGGAAAGGGAAGATTCAATAAGCAGAGGAAGGAGGTCACCAAGC AAGCCGGACTTCCTCTACAAAAAGTCTGCCCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001289133
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289133.1, NP_001276062.1
RefSeq Size	7233 bp
RefSeq ORF	2346 bp
Locus ID	55727
UniProt ID	Q9P203
Gene Summary	Acts as a mediator of epithelial dynamics and organ branching by promoting cleft progression. Induced following accumulation of fibronectin in forming clefts, leading to local expression of the cell-scattering SNAIL2 and suppression of E-cadherin levels, thereby altering cell morphology and reducing cell-cell adhesion. This stimulates cell separation at the base of forming clefts by local, dynamic intercellular gap formation and promotes cleft progression (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.