CDH18 (NM_001291956) Human Tagged ORF Clone
CAT#: RC239568
- TrueORF®
CDH18 (myc-DDK-tagged) - Human cadherin 18, type 2 (CDH18), transcript variant 3
ORF Plasmid: tGFP
"NM_001291956" in other vectors (2)
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CNY 5,784.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CDH14; CDH14L; CDH24 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239568 representing NM_001291956
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATTACTAGCACATCTTGCATCTGTCCAGTCCTAGTGTGTCTCTGTTTTGTGCAGAGGTGTTATG GAACTGCTCACCACAGCTCCATCAAGGTGATGAGAAACCAAACCAAACACATTGAAGGTGAAACCGAAGT CCATCATCGTCCCAAAAGGGGATGGGTATGGAATCAGTTCTTTGTTTTAGAAGAACATATGGGACCAGAT CCTCAGTATGTTGGAAAGCTGCACTCCAATTCTGACAAAGGTGATGGATCTGTCAAGTACATCCTTACTG GAGAGGGTGCTGGGACTATATTTATCATTGACGATACCACGGGTGATATCCACTCAACAAAAAGCCTAGA CAGAGAGCAGAAGACCCACTATGTGCTTCATGCTCAAGCTATTGATAGACGTACAAACAAACCTCTTGAG CCTGAATCCGAGTTCATCATCAAAGTGCAAGACATCAATGACAACGCTCCAAAATTCACAGATGGACCAT ACATTGTTACTGTGCCTGAAATGTCAGATATGGGTACCTCTGTTCTACAGGTGACAGCTACTGATGCAGA TGACCCTACCTATGGAAACAGCGCTCGGGTGGTTTACAGCATTCTCCAGGGACAACCCTACTTCTCCGTC GACCCTAAAACAGGAGTTATTAGAACGGCCTTACATAACATGGACGGAGAAGCCAGAGAACATTACTCCG TAGTCATTCAAGCCAAAGACATGGCTGGGCAAGTTGGAGGGCTTTCAGGATCTACAACAGTCAACATCAC CTTAACCGATGTCAATGACAACACACCACGCTTTCCTCAAAAACACTATCAGCTATATGTTCCTGAGTCA GCTCAAGTTGGTTCAGCTGTTGGGAAAATCAAGGCAAATGATGCTGACACTGGCTCAAATGCTGACATGA CCTACTCCATCATAAATGGTGATGGCATGGGAATATTCTCAATCTCCACTGACAAAGAGACCAGAGAAGG AATCCTTTCTTTAAAGAAGCCACTGAACTATGAGAAAAAGAAGTCATATACCCTCAACATAGAAGGAGCA AATACACATCTTGATTTTCGCTTTTCTCACTTGGGTCCTTTTAAAGATGCTACTATGCTGAAGATCATTG TTGGGGATGTAGATGAACCACCACTATTTTCCATGCCTTCCTACCTCATGGAAGTCTACGAAAATGCCAA GATTGGGACCGTCGTTGGTACAGTTTTGGCACAAGATCCTGACAGTATTAACAGCTTAGTAAGATACTTC ATCAACTACAATGTTGAAGACGACAGATTTTTCAACATTGATGCCAATACTGGGACCATTAGGACTACAA AGGTTCTCGACAGAGAAGAAACTCCATGGTACAACATCACAGTCACTGCTTCAGAAATTGATAATCCTGA TTTGCTGAGCCATGTCACAGTGGGTATTAGAGTTCTGGATGTCAATGACAATCCACCCGAACTTGCCAGG GAATATGATATTATTGTATGTGAAAATTCTAAGCCTGGCCAGGTTATTCATACCATCAGTGCCACTGATA AAGATGATTTTGCCAATGGACCAAGGTTTAACTTCTTTCTTGATGAGCGCCTGCCTGTAAATCCAAACTT CACTCTGAAGGACAATGAAGATAACACAGCCAGCATTCTGACAAGGCGGAGGAGATTTAGTCGAACTGTT CAGGATGTGTATTATCTGCCCATTATGATCTCTGATGGTGGAATCCCCTCTCTCAGCAGCAGCAGCACCC TCACCATCAGGGTTTGTGCATGCGAGAGAGATGGGCGTGTGCGGACCTGCCATGCAGAAGCCTTCCTGTC CTCGGCTGGTTTGAGTACAGGAGCCTTAATCGCTATTCTTCTCTGTGTTCTCATTCTCCTGGCAATTGTG GTACTTTTTATCACCCTGAGGCGCAGCAAAAAAGAGCCCTTGATCATTTCAGAAGAGGATGTACGGGAGA ACGTGGTCACCTATGATGATGAAGGAGGCGGAGAGGAAGACACAGGGGCCTTTGACATCACAGCCTTGAG GAATCCTTCTGCTGCTGAGGAGCTCAAGTACCGGAGGGATATCAGACCTGAAGTGAAGCTCACTCCCAGA CACCAGACATCATCCACCCTGGAAAGCATAGATGTTCAGGAATTTATTAAGCAAAGACTGGCAGAAGCAG ACCTAGACCCTAGCGTTCCCCCTTATGACTCTCTTCAGACTTATGCCTATGAGGGTCAGAGATCAGAAGC TGGGTCTATCAGCTCGCTGGATTCAGCAACGACACAATCAGACCAGGATTATCACTACCTTGGAGACTGG GGACCCGAGTTTAAAAAGTTAGCTGAACTCTATGGAGAAATAGAATCTGAAAGAACAACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239568 representing NM_001291956
Red=Cloning site Green=Tags(s) MKITSTSCICPVLVCLCFVQRCYGTAHHSSIKVMRNQTKHIEGETEVHHRPKRGWVWNQFFVLEEHMGPD PQYVGKLHSNSDKGDGSVKYILTGEGAGTIFIIDDTTGDIHSTKSLDREQKTHYVLHAQAIDRRTNKPLE PESEFIIKVQDINDNAPKFTDGPYIVTVPEMSDMGTSVLQVTATDADDPTYGNSARVVYSILQGQPYFSV DPKTGVIRTALHNMDGEAREHYSVVIQAKDMAGQVGGLSGSTTVNITLTDVNDNTPRFPQKHYQLYVPES AQVGSAVGKIKANDADTGSNADMTYSIINGDGMGIFSISTDKETREGILSLKKPLNYEKKKSYTLNIEGA NTHLDFRFSHLGPFKDATMLKIIVGDVDEPPLFSMPSYLMEVYENAKIGTVVGTVLAQDPDSINSLVRYF INYNVEDDRFFNIDANTGTIRTTKVLDREETPWYNITVTASEIDNPDLLSHVTVGIRVLDVNDNPPELAR EYDIIVCENSKPGQVIHTISATDKDDFANGPRFNFFLDERLPVNPNFTLKDNEDNTASILTRRRRFSRTV QDVYYLPIMISDGGIPSLSSSSTLTIRVCACERDGRVRTCHAEAFLSSAGLSTGALIAILLCVLILLAIV VLFITLRRSKKEPLIISEEDVRENVVTYDDEGGGEEDTGAFDITALRNPSAAEELKYRRDIRPEVKLTPR HQTSSTLESIDVQEFIKQRLAEADLDPSVPPYDSLQTYAYEGQRSEAGSISSLDSATTQSDQDYHYLGDW GPEFKKLAELYGEIESERTT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
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Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001291956 |
ORF Size | 2370 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001291956.3 |
RefSeq Size | 3559 bp |
RefSeq ORF | 2373 bp |
Locus ID | 1016 |
UniProt ID | Q13634 |
Protein Families | Transmembrane |
MW | 87.9 kDa |
Gene Summary | This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is expressed specifically in the central nervous system and is putatively involved in synaptic adhesion, axon outgrowth and guidance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014] |
Documents
Product Manuals |
FAQs |
SDS |