TLE4 (NM_001282748) Human Tagged ORF Clone
CAT#: RC239600
- TrueORF®
TLE4 (myc-DDK-tagged) - Human transducin-like enhancer of split 4 (TLE4), transcript variant 1
ORF Plasmid: tGFP
"NM_001282748" in other vectors (2)
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CNY 5,800.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BCE-1; BCE1; E(spI); E(spl); ESG; ESG4; Grg-4; GRG4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239600 representing NM_001282748
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATTCGCGACCTGAGCAAGATGTACCCGCAGACCAGACACCCGGCACCGCATCAGCCTGCTCAACCCT TTAAATTTACAATTTCCGAATCCTGTGATCGGATTAAGGAAGAGTTTCAGTTTTTACAGGCTCAATACCA CAGTCTGAAGCTGGAATGTGAGAAACTCGCCAGTGAGAAGACAGAGATGCAGCGGCATTATGTCATGTAT TATGAAATGTCCTATGGGTTGAATATAGAAATGCACAAGCAGGCAGAGATTGTCAAGAGGCTGAATGCTA TCTGTGCACAAGTCATTCCTTTCCTGTCCCAAGAGCACCAGCAACAAGTGGTGCAGGCTGTGGAACGGGC CAAGCAGGTGACCATGGCAGAACTGAACGCCATCATTGGGCAACAACTCCAGGCCCAGCATTTATCACAT GGACATGGTCTCCCCGTACCTCTGACTCCACACCCTTCAGGGCTCCAGCCCCCTGCCATTCCACCCATCG GTAGCAGTGCCGGGCTTCTGGCCCTCTCCAGTGCTCTAGGAGGTCAGTCCCATCTTCCAATTAAAGATGA GAAGAAGCACCATGACAATGATCACCAAAGAGACAGAGACTCCATCAAGAGCTCTTCAGTATCCCCATCA GCCAGTTTCCGAGGTGCTGAGAAGCACAGAAACTCCGCAGACTACTCCTCAGAGAGCAAAAAGCAGAAAA CTGAAGAAAAGGAAATTGCAGCTCGTTATGACAGCGATGGTGAGAAAAGTGATGACAACTTGGTGGTTGA CGTTTCCAATGAGGATCCATCTTCCCCTCGAGGGAGCCCAGCACATTCCCCCAGAGAGAATGGCCTAGAC AAGACACGCCTGCTCAAGAAAGATGCCCCGATTAGTCCAGCCTCTATTGCATCTTCCAGCAGTACTCCCT CCTCCAAATCCAAAGAACTTAGCCTTAAGAGGGATATGGGGAAATTGAGTGAAACACGTCTTAGCGAAGA TGAACAATGCACATTGGGGTTACAGAGATGGTTTTGTCGCCTGTGGTTTATGAATGAAAAATCTACTACT CCCGTCTCAAAGTCCAATACCCCTACTCCACGAACTGATGCGCCCACCCCAGGCAGTAACTCTACTCCCG GATTGAGGCCTGTACCTGGAAAACCACCAGGAGTTGACCCTTTGGCCTCAAGCCTAAGGACCCCAATGGC AGTACCTTGTCCATATCCAACTCCATTTGGGATTGTGCCCCATGCTGGAATGAACGGAGAGCTGACCAGC CCCGGAGCGGCCTACGCTGGGCTCCACAACATCTCCCCTCAGATGAGCGCAGCTGCTGCCGCCGCCGCTG CTGCTGCTGCCTATGGGAGATCACCAGTGGTGGGATTTGATCCACACCATCACATGCGTGTGCCAGCAAT ACCTCCAAACCTGACAGGCATTCCAGGAGGAAAACCAGCATACTCCTTCCATGTTAGCGCAGATGGTCAG ATGCAGCCTGTCCCTTTTCCACCCGACGCCCTCATCGGACCTGGAATCCCCCGGCATGCTCGCCAGATCA ACACCCTCAACCACGGGGAGGTGGTGTGCGCGGTGACCATCAGCAACCCCACGAGACACGTGTACACGGG TGGGAAGGGCTGCGTCAAGGTCTGGGACATCAGCCACCCAGGCAATAAGAGTCCTGTCTCCCAGCTCGAC TGTCTGAACAGGGATAACTACATCCGTTCCTGCAGATTGCTCCCTGATGGTCGCACCCTAATTGTTGGAG GGGAAGCCAGTACTTTGTCCATTTGGGACCTGGCGGCTCCAACCCCACGCATCAAGGCAGAGCTGACATC CTCGGCCCCCGCCTGCTATGCCCTGGCCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGAC GGCAACATCGCTGTGTGGGATCTGCACAACCAGACCTTGGTGAGGCAATTCCAGGGCCACACAGATGGAG CCAGCTGTATTGACATTTCTAATGATGGCACCAAGCTCTGGACAGGTGGTTTGGACAACACGGTCAGGTC CTGGGACCTGCGCGAGGGGCGGCAGCTGCAGCAGCACGACTTCACCTCCCAGATCTTTTCTCTGGGCTAC TGCCCAACTGGAGAGTGGCTTGCAGTGGGGATGGAGAACAGCAATGTGGAAGTTTTGCATGTCACCAAGC CAGACAAATACCAACTACATCTTCATGAGAGCTGTGTGCTGTCGCTCAAGTTTGCCCATTGTGGCAAATG GTTTGTAAGCACTGGAAAGGACAACCTTCTGAATGCCTGGAGAACACCTTATGGGGCCAGTATATTCCAG TCCAAAGAATCCTCATCGGTGCTTAGCTGTGACATCTCCGTGGACGACAAATACATTGTCACTGGCTCTG GGGATAAGAAGGCCACAGTTTATGAAGTTATTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239600 representing NM_001282748
Red=Cloning site Green=Tags(s) MIRDLSKMYPQTRHPAPHQPAQPFKFTISESCDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMY YEMSYGLNIEMHKQAEIVKRLNAICAQVIPFLSQEHQQQVVQAVERAKQVTMAELNAIIGQQLQAQHLSH GHGLPVPLTPHPSGLQPPAIPPIGSSAGLLALSSALGGQSHLPIKDEKKHHDNDHQRDRDSIKSSSVSPS ASFRGAEKHRNSADYSSESKKQKTEEKEIAARYDSDGEKSDDNLVVDVSNEDPSSPRGSPAHSPRENGLD KTRLLKKDAPISPASIASSSSTPSSKSKELSLKRDMGKLSETRLSEDEQCTLGLQRWFCRLWFMNEKSTT PVSKSNTPTPRTDAPTPGSNSTPGLRPVPGKPPGVDPLASSLRTPMAVPCPYPTPFGIVPHAGMNGELTS PGAAYAGLHNISPQMSAAAAAAAAAAAYGRSPVVGFDPHHHMRVPAIPPNLTGIPGGKPAYSFHVSADGQ MQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVTISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLD CLNRDNYIRSCRLLPDGRTLIVGGEASTLSIWDLAAPTPRIKAELTSSAPACYALAISPDSKVCFSCCSD GNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGY CPTGEWLAVGMENSNVEVLHVTKPDKYQLHLHESCVLSLKFAHCGKWFVSTGKDNLLNAWRTPYGASIFQ SKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001282748 |
ORF Size | 2415 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001282748.1, NP_001269677.1 |
RefSeq Size | 5174 bp |
RefSeq ORF | 2418 bp |
Locus ID | 7091 |
UniProt ID | Q04727 |
Protein Families | Transcription Factors |
MW | 88.2 kDa |
Gene Summary | Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |