ZNF281 (NM_001281294) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GZP1; ZBP-99; ZBP99; ZNP-99
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239697 representing NM_001281294 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATCGGCAGTGGGTTCCTGAGTGGCGGCGGAGGTACCGGCAGTAGCGGTGGTAGCGGCTCCGGCG GCGGTGGTAGTGGCGGCGGCGGCGGCGGCGGCAGCAGCGGCAGGAGGGCAGAGATGGAACCCACCTTTCC CCAGGCCCCGGCCGCTGAGCCCCCCCCTCCGCCAGCCCCGGACATGACTTTCAAGAAGGAGCCGGCGGCG TCAGCCGCGGCCTTCCCCTCGCAGAGGACCTCCTGGGGGTTCTTGCAGTCTTTGGTTAGCATCAAACAGG AGAAACCCGCGGATCCTGAGGAGCAGCAGTCCCACCACCACCATCACCACCACCACTATGGGGGGCTGTT CGCTGGAGCTGAAGAGAGGTCTCCAGGCCTAGGAGGCGGTGAAGGGGGGAGTCACGGCGTCATCCAGGAC CTCAGTATTCTCCACCAGCATGTCCAGCAGCAACCAGCCCAGCACCACCGTGACGTATTACTCAGCAGCA GTAGCAGGACTGATGACCACCATGGCACTGAGGAGCCAAAGCAGGACACTAATGTCAAAAAGGCAAAAAG GCCAAAGCCAGAATCTCAGGGAATCAAAGCCAAGAGGAAGCCAAGTGCATCTTCCAAACCTTCTTTGGTT GGAGATGGAGAAGGTGCCATCCTCTCCCCAAGTCAGAAACCTCATATCTGTGATCACTGTAGTGCTGCTT TCCGAAGCTCCTATCACCTGCGGAGACATGTCCTCATTCATACAGGAGAAAGACCTTTCCAGTGCAGCCA GTGTAGTATGGGTTTCATTCAGAAATACCTACTACAGAGACATGAGAAAATTCATAGTAGAGAGAAGCCA TTTGGATGTGATCAGTGCAGCATGAAGTTTATTCAGAAGTACCATATGGAGAGACACAAGAGGACACATA GTGGAGAAAAGCCATATAAGTGTGACACTTGCCAACAGTATTTTTCAAGGACTGATAGATTGTTGAAGCA CAGGCGCACATGTGGTGAAGTCATAGTTAAAGGAGCCACTAGTGCAGAACCTGGGTCATCAAACCATACC AATATGGGTAATCTGGCTGTGTTGTCTCAGGGAAATACAAGTTCTTCAAGGAGAAAAACAAAGTCAAAAA GCATAGCTATTGAAAATAAGGAACAGAAGACCGGTAAAACAAATGAATCGCAAATTTCAAATAATATAAA CATGCAGAGTTACTCAGTAGAAATGCCTACCGTGTCTTCCAGTGGAGGCATAATTGGCACTGGAATAGAT GAACTGCAGAAGAGGGTGCCAAAATTGATCTTTAAGAAAGGAAGCAGAAAGAATACAGATAAAAACTACC TTAACTTTGTGTCACCATTACCAGACATAGTAGGACAGAAATCCTTGTCTGGAAAACCAAGTGGCTCACT TGGCATAGTATCAAATAATAGTGTGGAGACCATTGGTCTTCTCCAAAGTACAAGTGGCAAACAAGGTCAG ATAAGTAGTAATTATGATGATGCCATGCAGTTTTCAAAGAAAAGAAGATATTTACCAACTGCCAGCAGCA ACAGTGCCTTTTCTATAAACGTAGGACACATGGTCTCCCAACAGTCTGTCATTCAGTCTGCAGGTGTCAG TGTTTTGGACAATGAGGCACCATTGTCACTTATTGACTCCTCAGCTCTAAATGCTGAAATTAAATCTTGT CATGACAAGTCTGGAATTCCTGATGAGGTTTTACAAAGTATTTTGGATCAATACTCCAACAAATCAGAAA GCCAGAAAGAGGATCCTTTCAATATTGCAGAACCACGAGTGGATTTACACACCTCAGGAGAACACTCAGA ATTGGTTCAAGAAGAAAATTTGAGCCCAGGCACCCAAACACCTTCAAATGATAAAGCAAGTATGTTGCAA GAATACTCCAAATACCTCCAACAGGCTTTTGAAAAATCCACTAATGCAAGTTTTACTCTTGGACACGGTT TCCAATTTGTCAGTTTGTCTTCACCTCTCCACAACCACACTTTGTTTCCAGAAAAACAAATATACACTAC GTCTCCTTTGGAGTGTGGTTTCGGCCAATCTGTTACCTCAGTGTTGCCATCTTCATTGCCAAAGCCTCCT TTTGGGATGTTGTTTGGATCTCAGCCAGGTCTTTATTTGTCTGCTTTGGATGCTACACATCAGCAGTTGA CACCTTCCCAGGAGCTGGATGATCTGATAGATTCTCAGAAGAACTTAGAGACTTCATCAGCCTTCCAGTC CTCATCTCAGAAATTGACTAGCCAGAAGGAACAGAAAAACTTAGAGTCTTCAACAGGCTTTCAGATTCCA TCTCAGGAGTTAGCTAGCCAGATAGATCCTCAGAAAGACATAGAGCCTAGAACAACGTATCAGATTGAGA ACTTTGCACAAGCGTTTGGTTCTCAGTTTAAGTCGGGCAGCAGGGTGCCAATGACCTTTATCACTAACTC TAATGGAGAAGTGGACCATAGAGTAAGGACTTCAGTGTCAGATTTCTCAGGGTATACAAATATGATGTCT GATGTAAGTGAGCCATGTAGTACAAGAGTAAAGACACCCACCAGCCAGAGTTACAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239697 representing NM_001281294 Red=Cloning site Green=Tags(s) MKIGSGFLSGGGGTGSSGGSGSGGGGSGGGGGGGSSGRRAEMEPTFPQAPAAEPPPPPAPDMTFKKEPAA SAAAFPSQRTSWGFLQSLVSIKQEKPADPEEQQSHHHHHHHHYGGLFAGAEERSPGLGGGEGGSHGVIQD LSILHQHVQQQPAQHHRDVLLSSSSRTDDHHGTEEPKQDTNVKKAKRPKPESQGIKAKRKPSASSKPSLV GDGEGAILSPSQKPHICDHCSAAFRSSYHLRRHVLIHTGERPFQCSQCSMGFIQKYLLQRHEKIHSREKP FGCDQCSMKFIQKYHMERHKRTHSGEKPYKCDTCQQYFSRTDRLLKHRRTCGEVIVKGATSAEPGSSNHT NMGNLAVLSQGNTSSSRRKTKSKSIAIENKEQKTGKTNESQISNNINMQSYSVEMPTVSSSGGIIGTGID ELQKRVPKLIFKKGSRKNTDKNYLNFVSPLPDIVGQKSLSGKPSGSLGIVSNNSVETIGLLQSTSGKQGQ ISSNYDDAMQFSKKRRYLPTASSNSAFSINVGHMVSQQSVIQSAGVSVLDNEAPLSLIDSSALNAEIKSC HDKSGIPDEVLQSILDQYSNKSESQKEDPFNIAEPRVDLHTSGEHSELVQEENLSPGTQTPSNDKASMLQ EYSKYLQQAFEKSTNASFTLGHGFQFVSLSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPP FGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQKNLESSTGFQIP SQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMS DVSEPCSTRVKTPTSQSYR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281294
ORF Size	2577 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281294.2
RefSeq Size	4800 bp
RefSeq ORF	2580 bp
Locus ID	23528
UniProt ID	Q9Y2X9
Protein Families	ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transcription Factors
MW	93.7 kDa
Gene Summary	Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.[UniProtKB/Swiss-Prot Function]

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