ZNF281 (NM_001281294) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GZP1; ZBP-99; ZBP99; ZNP-99
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001281294, the custom clone sequence may differ by one or more nucleotides ATGAAAATCGGCAGTGGGTTCCTGAGTGGCGGCGGAGGTACCGGCAGTAGCGGTGGTAGCGGCTCCGGCG GCGGTGGTAGTGGCGGCGGCGGCGGCGGCGGCAGCAGCGGCAGGAGGGCAGAGATGGAACCCACCTTTCC CCAGGCCCCGGCCGCTGAGCCCCCCCCTCCGCCAGCCCCGGACATGACTTTCAAGAAGGAGCCGGCGGCG TCAGCCGCGGCCTTCCCCTCGCAGAGGACCTCCTGGGGGTTCTTGCAGTCTTTGGTTAGCATCAAACAGG AGAAACCCGCGGATCCTGAGGAGCAGCAGTCCCACCACCACCATCACCACCACCACTATGGGGGGCTGTT CGCTGGAGCTGAAGAGAGGTCTCCAGGCCTAGGAGGCGGTGAAGGGGGGAGTCACGGCGTCATCCAGGAC CTCAGTATTCTCCACCAGCATGTCCAGCAGCAACCAGCCCAGCACCACCGTGACGTATTACTCAGCAGCA GTAGCAGGACTGATGACCACCATGGCACTGAGGAGCCAAAGCAGGACACTAATGTCAAAAAGGCAAAAAG GCCAAAGCCAGAATCTCAGGGAATCAAAGCCAAGAGGAAGCCAAGTGCATCTTCCAAACCTTCTTTGGTT GGAGATGGAGAAGGTGCCATCCTCTCCCCAAGTCAGAAACCTCATATCTGTGATCACTGTAGTGCTGCTT TCCGAAGCTCCTATCACCTGCGGAGACATGTCCTCATTCATACAGGAGAAAGACCTTTCCAGTGCAGCCA GTGTAGTATGGGTTTCATTCAGAAATACCTACTACAGAGACATGAGAAAATTCATAGTAGAGAGAAGCCA TTTGGATGTGATCAGTGCAGCATGAAGTTTATTCAGAAGTACCATATGGAGAGACACAAGAGGACACATA GTGGAGAAAAGCCATATAAGTGTGACACTTGCCAACAGTATTTTTCAAGGACTGATAGATTGTTGAAGCA CAGGCGCACATGTGGTGAAGTCATAGTTAAAGGAGCCACTAGTGCAGAACCTGGGTCATCAAACCATACC AATATGGGTAATCTGGCTGTGTTGTCTCAGGGAAATACAAGTTCTTCAAGGAGAAAAACAAAGTCAAAAA GCATAGCTATTGAAAATAAGGAACAGAAGACCGGTAAAACAAATGAATCGCAAATTTCAAATAATATAAA CATGCAGAGTTACTCAGTAGAAATGCCTACCGTGTCTTCCAGTGGAGGCATAATTGGCACTGGAATAGAT GAACTGCAGAAGAGGGTGCCAAAATTGATCTTTAAGAAAGGAAGCAGAAAGAATACAGATAAAAACTACC TTAACTTTGTGTCACCATTACCAGACATAGTAGGACAGAAATCCTTGTCTGGAAAACCAAGTGGCTCACT TGGCATAGTATCAAATAATAGTGTGGAGACCATTGGTCTTCTCCAAAGTACAAGTGGCAAACAAGGTCAG ATAAGTAGTAATTATGATGATGCCATGCAGTTTTCAAAGAAAAGAAGATATTTACCAACTGCCAGCAGCA ACAGTGCCTTTTCTATAAACGTAGGACACATGGTCTCCCAACAGTCTGTCATTCAGTCTGCAGGTGTCAG TGTTTTGGACAATGAGGCACCATTGTCACTTATTGACTCCTCAGCTCTAAATGCTGAAATTAAATCTTGT CATGACAAGTCTGGAATTCCTGATGAGGTTTTACAAAGTATTTTGGATCAATACTCCAACAAATCAGAAA GCCAGAAAGAGGATCCTTTCAATATTGCAGAACCACGAGTGGATTTACACACCTCAGGAGAACACTCAGA ATTGGTTCAAGAAGAAAATTTGAGCCCAGGCACCCAAACACCTTCAAATGATAAAGCAAGTATGTTGCAA GAATACTCCAAATACCTCCAACAGGCTTTTGAAAAATCCACTAATGCAAGTTTTACTCTTGGACACGGTT TCCAATTTGTCAGTTTGTCTTCACCTCTCCACAACCACACTTTGTTTCCAGAAAAACAAATATACACTAC GTCTCCTTTGGAGTGTGGTTTCGGCCAATCTGTTACCTCAGTGTTGCCATCTTCATTGCCAAAGCCTCCT TTTGGGATGTTGTTTGGATCTCAGCCAGGTCTTTATTTGTCTGCTTTGGATGCTACACATCAGCAGTTGA CACCTTCCCAGGAGCTGGATGATCTGATAGATTCTCAGAAGAACTTAGAGACTTCATCAGCCTTCCAGTC CTCATCTCAGAAATTGACTAGCCAGAAGGAACAGAAAAACTTAGAGTCTTCAACAGGCTTTCAGATTCCA TCTCAGGAGTTAGCTAGCCAGATAGATCCTCAGAAAGACATAGAGCCTAGAACAACGTATCAGATTGAGA ACTTTGCACAAGCGTTTGGTTCTCAGTTTAAGTCGGGCAGCAGGGTGCCAATGACCTTTATCACTAACTC TAATGGAGAAGTGGACCATAGAGTAAGGACTTCAGTGTCAGATTTCTCAGGGTATACAAATATGATGTCT GATGTAAGTGAGCCATGTAGTACAAGAGTAAAGACACCCACCAGCCAGAGTTACAGGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281294
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281294.1, NP_001268223.1
RefSeq Size	4800 bp
RefSeq ORF	2580 bp
Locus ID	23528
UniProt ID	Q9Y2X9
Protein Families	ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transcription Factors
Gene Summary	Transcription repressor that plays a role in regulation of embryonic stem cells (ESCs) differentiation. Required for ESCs differentiation and acts by mediating autorepression of NANOG in ESCs: binds to the NANOG promoter and promotes association of NANOG protein to its own promoter and recruits the NuRD complex, which deacetylates histones. Not required for establishement and maintenance of ESCs (By similarity). Represses the transcription of a number of genes including GAST, ODC1 and VIM. Binds to the G-rich box in the enhancer region of these genes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region compared to variant 1. The resulting protein (isoform 2) is missing an internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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